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Volume ， Issue 2 ， 1993
- Abstract：<正> A hepatitis C virus（HCV）cDNA fragment,534bp in length and designated asQ534,was obtained by PCR amplification with self-designed primers.Q534 was cloned in-to Hinc Ⅱ site of pUC18 and the recombinant plasmid pQ534 was then selected from thebacterial transformants.The sequence analysis indicated that Q534 was a cDNA fragmentof HCV core gene,and located in HCV genome from positions 320 to 853 incorrespondence with Chiron’s prototype sequence.The homologies between Q534 and theprototype at the levels of nucleotides and amino acids were 90.0% and 97.6%,respectively.The homologies of Q534 with Japanese HCV-J and HCV-BK strains were 96.6% and97.0% at the nucleotide level,and 98.2% and 98.8% at the amino acid level.In terms ofthe sequence,this Chinese HCV isolate should belong to HCV group Ⅱ.0|12|1Updated：2026-03-12
- Abstract：<正> A preliminary study on the serum hepatitis C virus（HCV）RNA in 182 pa-tients with different chronic liver diseases and 35 blood donors was carried out with“nested”polymerase chain reaction.It was found that serum HCV RNA was detected in36.6%（34/93）cases of primary hepatic carcinoma（PHC）,31.7%（20/63）liver cirrhosis,15.4%（4/26）chronic hepatitis and 2.9%（1/35）blood donors;most of the HCV RNA pos-itive cases（41/58）were complicated with HBV replicating markers;the rate of single posi-tive for HCV RNA was 15.1%（14/93）in PHC,3.2%（2/63）in liver cirrhosis,and 3.8%（1/26）in chronic hepatitis.These findings imply that about 20% of PHC cases appear tobe related to the coinfection of HCV and HBV and 15% of PHC cases are related to sin-gle HCV infection.0|3|1Updated：2026-03-12
- Abstract：<正> Using a modification of the very sensitive glucose-DAB-nickel（GDN）immunohistochemistry method,FOS（the expression protein of the oncogene c-fos）-,neuropeptide Y（NPY）-,somatostatin（SOM）-,leu-enkephalin（L-Enk）,cholecystokinin（CCK）-,neurotensin（NT）-,and tyrosine hydroxylase（TH）-immunoreactivities were firstdemonstrated in the protozoan,Stylonychia mytilus.The GDN method and expression ofFOS,neuropeptides and TH in the Stylonychia mytilus were discussed.0|17|0Updated：2026-03-12
- Abstract：<正> The homogeneity between the high-and low-affinity glucocorticoid receptors（GRH and GRL）was testified by immunoaffinity chromatography using Mab N250（recognizing the immunogenic domain at the N terminal of GRH）and Mab BuGR1（recognizing the DNA binding domain of GRH）.The specific binding peak of 0.98μmol/L[3H]triamcinolone acetonide（TA）was higher than that of 52.50nmol/L[3H]TA.The re-sult suggests that the antigenic determinants of GRL are similar to those of GRH.ThecDNA of rat liver GRH was introduced into GRH-and GRL-negative mouse fibroblast cellline E82.A3 by calcium phosphate coprecipitation.A number of clones which expressGRH were selected with G418（400μg/ml）.The results of radioligand binding assay indicatethat GRH gene is expressed successfully and GRL also may be encoded by GRH gene.0|5|0Updated：2026-03-12
- Abstract：<正> One hind leg（about 7% TBSA）of a rat was scalded and the changes of thereduction-oxidation state and protein degradation in the soleus muscle were observed inthe 72nd h after scalding both in vitro and in vivo.It was found that the lactate/pyruvate（L/P）and malate/pyruvate（M/P）ratios in the soleus muscle were significantly lower andthe protein degradation rate significantly higher in the scalded rats than those in the controland in the unscalded legs.After the addition of insulin to the medium,significant eleva-tion of L/P and M/P ratios and reduction of the protein degradation rate were observedin the soleus muscle.These findings suggest that there is a good correlation between thechanges of the reduction-oxidation and the protein degradation rate in the cytosol of thesoleus muscle after scalding in rats.0|3|0Updated：2026-03-12
- Abstract：<正> Anti-hepatoma monoclonal antibodies HAb8,HAb18 IgG and HAb18 F（ab’）2were labelled with[131I]according to chloramine T method.The labelling rate andradioactivity were 51% and 9.58×104Bq/mg,respectively.After administration of the[131I]-labelled conjugates through mouse tail vein,hepatoma xenografts were clearly im-aged,Localization index was 2.15,3.09,and 6.99 for HAb8,HAb18 IgG and HAb18F（ab’）2,respectively.A total of 16 hepatoma-bearing nude mice（SMMU-LTNM）were di-vided into 4 groups.When xenografts reached 0.3cm in diameter,the treatment was be-gun by injection of the[131I]-conjugates through mouse tail vein.After 2 weeks of treat-ment,obvious inhibitory or killing effects on the xenografts were found.The tu-mor-bearing mice were killed during the period from 14 to 21d after treatment.Xenografts as well as other organs were sampled and viewed under light microscope ac-cording to routine procedure.The vital organs,such as heart,liver,spleen,lung,kidneyand brain showed no pathological lesions.In hepatoma xenografts,some tumor cellmembranes were found,with obscure cytoplasmic structure and increased eosinophilia,representing necrotic alterations.0|11|1Updated：2026-03-12
- Abstract：<正> Using immunohistochemical technique,the cell bodies and fiber terminals of 13peptidergic neurons-vasoactive intestinal peptide（VIP）-,cholecystokinin（CCK）-,corticotropin releasing factor（CRF）-,neurotensin（NT）-,galanin（GAL）-,substance P（SP）-,somatostatin（SOM）-,calcitonin gene related peptide（CGRP）-,α-melanocytestimulating hormone（β-MSH）-,β-endorphin（β-END）-,angiotensin Ⅱ（Ang Ⅱ）-,L-enkephalin（L-ENK）-,and M-enkephalin（M-ENK）-immunohistochemical reactive（IR）neurons were studied and the pattern of distribution of these peptidergic neurons inthe amygdaloid nucleus（AMYG）and the bed nucleus of the stria terminalis（BST）of therat was compared.Some similarities of the distribution of these peptidergic neurons in thesubdivisions of the AMYG and BST were observed.The peptidergic neurons in the ante-rior part of BST are similar to those in the central amygdaloid nucleus and in the medialamygdaloid nucleus,and the peptidergic neurons in the posterior part of BST are similarto those in the lateral and basal amygdaloid nucleus.0|7|0Updated：2026-03-12
- Abstract：<正> The left cheek of 8 adult male mongrel dogs was shot with M1935.56mm bul-let fired through an US-made M16 rifle to result in a perforating soft tissue defect.Pri-mary debridement was performed immediately and the secondary debridement 72h alter in-jury.After the secondary thorough debridement,the cutaneous side of the defect was re-paired with a saphenous artery-vein free skin（muscular）flap using microvascularanastomosis,and the oral side of the defect was repaired by drawing the surroundingmucosa together and fixing with sutures or with a local sliding mucous membrane flap.All the animals were kept under observation for 2～6 months.It was believed that the pri-mary debridement and secondary thorough debridement were both essential. The survivalrate of the free tissue flap was 75%（6/8）.These facts suggest that early repair ofthe oromaxillofacial perforating soft tissue defect due to gunshot is practicable.0|2|0Updated：2026-03-12
- Abstract：<正> Stress fracture（SF）in military recruits in China was investigated using99mTc-MDP bone scintigraphy in a randomized sample of 432 injured who wereclinically screened from 1060 recruits during basic training.The relationship between SFand training intensity was discussed.It was shown that the total incidence was 32.5%.There were two peaks during 9 training-weeks:one was in the 2nd week,and the otherin the 7th.Infantry soldiers had a more intensive training and,therefore a higher inci-dence than in artillery and service troops.Although SF was found in most ofweight-bearing bones,tibia was most commonly fractured（67%）.The findings suggestthat SF is a major training-related injury in military recruits and improperly strengthenedtraining can increase the incidence.0|11|0Updated：2026-03-12
- Abstract：<正> The biomodulative and hematopoietic potentialities of IL-2 and IL-3 activatedbone marrow（ABM）cells from patients with lung adenocarcinoma were studied in vitro.Human bone marrow（BM）cells could be activated by IL-2 in culture for 7d.TheseIL-2 ABM cells had higher cytolytic activities against cells of H 7402 cell line and freshautologous adenocarcinoma cells and maintained the cytotoxicities longer than IL-2 acti-vated peripheral blood lymphocytes（APBLs）,a point of possible importance in adoptiveimmunotherapy for cancer patients.The IL-2 ABM cells also had similar number ofBFU-E and CFU-GM to that had fresh BM cells if 1L-3 was added 48h alter IL-2 inculture.The IL-2 and IL-3 ABM cells might be used to eliminate tumor cells and tosupply reconstitutive elements of BM for autologous bone marrow transplantation.0|11|0Updated：2026-03-12
- Abstract：<正> The arterial supply of the genicular meniscus was studied with macro-microanatomical and histological examinations,translucent preparations and scanning electronmicroscopy.It was found that the menisci were supplied by the articular branches of thepopliteal artery except the lateral superior genicular branch and the descending genicularartery.Among them there were two genicular branches with independent stems from thepopliteal artery,named as the posterior medial and posterior lateral genicular artery,whichsupplied the posterior part of the corresponding meniscus.The genicular branchesanastomosed with each other to form a vascular circle around the menisci,and minutebranches from the circle formed an arterial network around the menisci and terminated incapillaries in 3 types of anastomotic forms.The surgical approach to the menisci and theprognosis of an injured meniscus were discussed on the basis of the arterial distributionsinside and outside of the menisci.0|6|0Updated：2026-03-12
- Abstract：<正> Peripheral blood mononuclear cells（PBMNC）isolated from patients with acuteleukemia（AL）and from normal controls were cultured in a medium containing1000units/ml of recombinant interleukin-2（IL-2）.Marked LAK activity was induced onthe third culture day in the normal controls,with the highest cytotoxicity appearing be-tween day 3 and 5,whereas induction of LAK activity in the AL patients began on the5th day of culture,with a peak level appearing at day 15,showing that the peak ofLAK activity was significantly delayed in AL.LAK cells surface phenotyping tests showedthat CD8 and CD16 positive cells began to increase significantly from day 5 and reachedthe highest level at week 3,whereas CD4- subclass began to decrease at day 5 anddropped to the nadir at week 3,indicating that LAK activity was positively correlatedwith the proportion of CD8+ and CD16+ cells,but negatively with that of CD4+ cells.0|7|0Updated：2026-03-12
- Abstract：<正> Changes of 5-hydroxytryptamine（5-HT）and 5-hydroxyindoleacetic acid（5-HIAA）levels in brain cortex and serum were studied using spectrofluorometer follow-ing brain injury in rats.Cyproheptadine,a receptor antagonist of 5-HT,was employedand its effect on alterations of 5-HT and 5-HIAA contents in brain cortex and serum af-ter brain injury was investigated.The results showed that after brain injury levels of5-HT and 5-HIAA in the brain cortex increased markedly,The increase of 5-HT in thebrain cortex reached its peak at 48h after brain injury,and its value was 4.29±0.44μg/g,which was 4.8 times of control value.The increase of 5-HIAA in the brain cortexreached its peak at 24h after brain injury,and its value was 5.48±0.41μg/g,which was5.8times of control value.The change of 5-HT in the serum was not significant,but thelevel of 5-HIAA in the serum was increased significantly.Cyproheptadine could reducelevels of 5-HT and 5-HIAA in this experiment.The value of 5-HT in the brain cortexdecreased to 1.32±0.09μg/g,and the value of 5-HIAA decreased to 1.36±0.10μg/g.Thelevel of 5-HIAA in the serum was also reduced significantly.It was 0.27±0.03μg/ml.0|11|0Updated：2026-03-12
- Abstract：<正> The relationship between 5-HT receptor and α-adrenoceptor was investigated inrat medullary slice preparations.In 57 nucleus tractus solitarii（NTS）neurons,47（82.5%）responded to both serotonin（5-HT）and norepinephrine（NE）.After synapse transmissionwas blocked by perfusion of artificial cerebrospinal fluid（ACSF）with low calcium andhigh magnesium,5 in 8 neurons were still sensitive to both 5-HT and NE.On the otherhand,3 in 5 neurons responding only to 5-HT or NA were sensitive to both 5-HT andNE after the synapse transmission blockage.In some neurons which exhibited excitatoryor inhibitory responses to 5-HT or NE alone,the responses were inversed when perfusedwith 5-HT and NE in combination.These results suggest that there may exist aninhibitory interaction between 5-HT receptors and α-adrenoceptors in NTS and that most5-HT and α-adrenoceptors coexist in the same NTS neurons.0|10|0Updated：2026-03-12
- Abstract：<正> We have previously shown that the lipofuscin in the brain seems to have in-creased in amount in autopsy cases of epidemic hemorrhagic fever.The purpose of thisstudy was to testify if there is really such an increase.Lipfuscin in 10 sections from everybrain of 10 autopsy cases,stained with Sudan Ⅳ,Sudan black and H.E.,was carefully es-timated and found to be greatly increased as compared with the controls of the same agewithout brain disease.Animal experiment was also conducted on 15 sucking BALB/c miceby I.P.inoculation of 100 LD50（0.05ml）of strain Chen of hemorrhagic fever virus,andon 15 mice without inoculation as controls.No lipofuscin was detected in the controls.However,in the brains of experimental mice,lipofuscin was found to be markedly in-creased,especially in the necrotic cells.The findings suggest that the over-productionand deposition of lipofuscin may be a mild change caused by the virus and its related fac-tors,which might be enhanced by hypotension and shock.0|9|0Updated：2026-03-12
- Abstract：<正> Modification was made on the reversed-phase high performance liquidchromatography（RP-HPLC）with Yue et al’s method as a base.The modified RP-HPLCwas used to detect leukotriene B4（LTB4）and 5-hydroxyeicosatetraenoic acid（5-HETE）.Itwas found that the modified method has the merits of simpler procedures,shortertesting time and more satisfactory efficacy.0|7|0Updated：2026-03-12
- Abstract：<正> This paper discusses how the positive and negative predictive values vary withcontrary variations of sensitivity and specificity at a certain disease prevalence.Let thesensitiviy be Se and the specificity be Sp at the initial cutoff point,correspondingly Se’and Sp’ at a new cutoff point:A=Se’/Se:B-（1-Sp’）/（1-Sp）:C=Sp’/Sp;D=（I-Se’）/（I-Se）.Moving the cutoff point from the initial point to the new point,if the contrary variationsof the sensitivity and specificity satisfy the inequality A>B,then the positive predictivevalue increases,otherwise it decreases or remains unchanged.If the variations satisfy theinequality C>D,then the negative prcdictive value increases,otherwise it decreasses or re-mains unchanged.0|21|0Updated：2026-03-12
- Abstract：<正> This paper reviewed 136 cases with definite bone and joint lesions selected froma total of 4286 burn patients.The clinical appearance,blood and urine biochemistry,blood cultures,gross and histological autopsy examination as well as surgical and biopsyspecimens were studied with emphasis on X-ray manifestations.The lesions were classi-fied,according to their X-ray appearances,into four types:alterations limited to thebone,alterations involving periarticular structures,changes of the joint and deformity ofthe bone and joint.The pathogenesis and radiological appearances of osteoporosis,mutila-tion of the fingers as well as incidence and pathogenesis of heterotopic para-articularossification were analysed and discussed.0|14|0Updated：2026-03-12
- Abstract：<正> Myringoplasty using exogenous collagen membrane was performed in 20 earsof 10 rabbits after complete removal of drumhead.Healing results were observed at 10,20,40and 60d after operation.Nineteen ears were transplanted successfully.Meanwhile,thehistology and ultrastructural morphology of the rabbit tympanic membranes were com-pared before and after transplantation.The results confirmed that the graft has a goodproperty of biocompatibility,and that it is useful in tympanic membrane transplantation.0|3|0Updated：2026-03-12
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