Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B<sub>4</sub> and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits

张敏; 尤忠义; 雷政宏; 王风君

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Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B₄ and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits

Updated：2026-03-12

- Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B₄ and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits
- Military Medical Research Issue 2, Pages: 192-196(1993)
- Affiliations：
  
  1. Burn Research Institute Southwest Hospital
  2. Third Military Medical College
  3. ,Chongqing,630038
- Author bio：
- Funds：
- DOI：
  CLC：
- Published：1993
- Accepted：
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[1]张敏,尤忠义,雷政宏,王风君.Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits[J].Journal of Medical Colleges of PLA,1993(02):192-196.
DOI：

[1]张敏,尤忠义,雷政宏,王风君.Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B_4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits[J].Journal of Medical Colleges of PLA,1993(02):192-196. DOI：

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Modification was made on the reversed-phase high performance liquidchromatography（RP-HPLC）with Yue et al’s method as a base.The modified RP-HPLCwas used to detect leukotriene B

（LTB

）and 5-hydroxyeicosatetraenoic acid（5-HETE）.Itwas found that the modified method has the merits of simpler procedures

shortertesting time and more satisfactory efficacy.

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Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B4 and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits

DOI：

摘要

Abstract

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Modified reversed-phase high performance liquid chromatography for the determination of leukotriene B₄ and 5-hydroxyeicosatetraenoic acid in peripheral neutrophils of rabbits