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Volume ， Issue 2 ， 2003
- Abstract：<正> The brief anesthesia with isoflurane induces rapid tolerance against focal cerebral ischemia in rats and aden-osine A1 receptor antagonist, DPCPX, attenuates the beneficial effect of isoflurane preconditioning.0|13|0Updated：2026-03-12
- Abstract：<正> Objective: To study the relationship between the expression of muscarinic receptor subtype M3R and thedecomposition of phosphatidylinositol（PI）. Methods: The[3H]-IP contents were detected with[3H]incorporationin human cultured detrusor muscle cells after being stimulated by carbachol, atropine, methoctramine, and 4-DAMP,respectively. Results: The [3H]-IP contents m increased with the elevation of carbachol concentration. Both atro-pine and 4-DAMP significantly inhibited the effect of carbachol on PI decomposition （P<0.01）, but methoctramine0|5|0Updated：2026-03-12
- Abstract：<正> Objective: To observe the oxidative modification of high density lipoprotein （HDL） induced by culturedhuman arterial smooth muscle cells （SMCs）. Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected,and native HDL （N-HDL） served as control. Oxidative modification of HDL was identified by using agarose gel electro-phoresis. Absorbances of conjugated diene （CD） and lipid hydroperoxide （LOOH） were measured with ultravioletspectrophotometry at 234 and 560 nm respectively, and fluorescence intensity of thiobarbuturic acid reaction substance0|12|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the relationship between oxidative stress and apoptosis and bcl-2 expression fol-lowing traumatic brain injury （TBI）. Methods: Male Sprague-Dawley ras were subjected to lateral fluid percussionbrain injury （FPBI） of moderate severity. U-74389G （20 mg/kg） were administered intravenously before FPBI. Theneurological functions were measured by beam-walk task （BWT） and beam-balance task （BBT）. In addition to mor-phological evidence of apoptosis, TUNEL histochemistry was used to identify DNA fragmentation in situ with both lightand electron microscopic levels. The internucleosomal fragments of DNA in apoptotic cells were examined using agarose0|19|0Updated：2026-03-12
- Abstract：<正> Objective: To study responses of injured DRG neurons to sympathetic stimulation with different temporalstimulating pattern. Methods: In chronically compressed DRG model, the spontaneous activity of single fiber from theinjured DRG neuron was recoeded, lumbar sympathetic trunk was electrical stimulated, and the responses of DRG neu-rons caused by chaotic and periodic sympathetic stimulation were compared. Results: It was found that the response of0|8|0Updated：2026-03-12
- Abstract：<正> Ojective: To investigate the effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on theproliferation of vascular smooth muscle cells （VSMCs）. Methods: Based on the computer analysis of the secondarystructure of c-myc mRNA, nt 2029 in rat c-myc oncogene was selected as a cleaving site for hammerhead ribozyme andthe ribozyme was designed. With automatic DNA synthesizer, the two complementary DNA strands of the ribozyme weresynthesized. The ribozyme gene was cloned into pGEM3Zf（+）vector and subcloned into eukaryotic expression pcD-NA3 vector. The recombinant pcDNA-Rz was transfected into the cultured rat VSMCs by lipofectAMINE mediated DNA0|14|0Updated：2026-03-12
- Abstract：<正> Objective: To assess the cerebral injury in on-pump beating-heart procedures under mild hypothermia incomparison with traditional on-pump arrested-heart procedures under moderate hypothermia. Methods: Forty patients,20 with congenital heart disease （CHD） and 20 of rheumatic heart disease （RHD）, were divided into 2 groups: Con-trol group （group A, n=20） including 10 patients suffering from CHD as group A1 and the left 10 from RHD as groupA2; and experiment group （group B, n=20） which consisting of group B1 （10 with CHD） and group B2 （10 ofRHD）. The patients in group A underwent traditional arrested-heart procedures, and those in group B were operated on0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the effect of thrombolysis with anticoagulation treatment for early stage of deep veinthrombosis of lower extremity. Methods: The clinical data of 106 patients at the early stage of deep vein thrombosis（DVT） in the lower extremities treated by thrombolysis with anticoagulation and dispersion drugs were analyzed retro-spectively. Results: The thrombolytic effect was significant. After treatment, the deep veins were recanalized withoutregurgitation in 75.3% of the patients. The total effective rate was 100%. Only three patients had hemorrhagic compli-0|15|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the relationship between C-reactive protein（CHP） concentration and severity of cor-onary artery lesion. Methods: The plasma CRP concentration were tested in 166 cases who underwent coronary angiog-raphy（CAG）,coronary heart disease （CHD） was presented in 86 patients and absent in 80 cases as normal controlgroup, and results of the two groups and different numbers of diseased coronary vessels in CHD group were analyzed.Results: The plasma CRP concentration were 2293±198, 5126±508,12969±1076μg/L, respectively,for the nor-mal control group, the stable angina group and the unstable angina group. The CRP concentration in the stable angina0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To uncover new clue for the research of the etiology of pregnancy-induced hypertension （PIH）by testing the gene expression difference between preeclamptic placentas and normal ones. Methods: mRNA level of 4PIH placentas were examined using 4000 feature cDNA microarray in comparison with the pooled control consisting oftotal RNA from 4 cases of PIH placentas after the control cDNA and experimental cDNA were labeled by cy3 and cy5respectively. Results: Fifty-eight to 131 genes were found down or up-regulated in 4 runs of hybridization. Among the0|22|0Updated：2026-03-12
- Abstract：<正> Objective: To study on the preparation process of a new surfactant-based microbubble ultrasound contrastagent and to evaluate its contrast effects in vivo. Methods: Microbubble ultrasound contrast agent with three ester sur-factants and other additives as its shell materials was prepared by sonication. Sulfur hexafluoride was adopted as the in-ner gas of the microbubbles. New methods through the combination of optical microscope and some softwares were usedto measure the size distribution and the concentration of the microbubbles. Some parameters such as the pH value of thephosphate buffer, quantity of the carboxylic methyl cellulose in the shell materials, selection of the ultrasound powerand process time, were studied. Six hybirded dogs were used to verify the in vivo contrast imaging of the contrast agentusing second harmonic power Doppler modality. Safety and persistent time of the agent inner animal body were also in-0|60|0Updated：2026-03-12
- Abstract：<正> Objective: To assess the effects of various anesthetic techniques and PaCO2 levels on cerebral oxygen sup-ply/consumption balance during craniotomy for removal of tumors, and to explore an anesthetic technique for neurosur-gery and an appropriate degree of PaCO2 during neuroanesthesia. Methods: One hundred and fourteen patients withsupratentorial tumors for elective craniotomy, ASA grade Ⅰ-Ⅱ, were randomly allocated to six groups. Patients wereanesthetized with continuous intravenous infusion of 2% procaine 1.0 mg·kg-1·min-1 in Group Ⅰ, inhalation of1.0%-1.5% isoflurane in Group Ⅱ, and infusion of 2% procaine 0.5 mg·kg-1·min-1 combined with inhalationof 0.5%-0.7% isoflurane in Group Ⅲ during the period of study. The end-tidal pressure of CO2（PET CO2）was0|9|2Updated：2026-03-12
- Abstract：<正> Objective: To study the effects of quercetin on hepatocyte stimulating factor production from mouse perito-neal macrophages. Methods: Hepatocyte stimulating factor was evaluated by the amount of fibrinogen synthesized inHep3B cells. Interleukin-6 activity was measured by B9 cell proliferation methyl thiazolyl tetrazolium colorimetric meth-od. Hep3B cell supernatant fibrinogen was quantitated with ELISA. Results: LPS induced the synthesis of hepatocytestimulating factor in mouse peritoneal macrophages, and hepatocyte stimulating factor promotes the synthesis of fibrino-gen from Hep3B cells. Quercetin（5 to 40 μmol/L） inhibited the synthesis of hepatocyte stimulating factor stimulated0|19|0Updated：2026-03-12
- Abstract：<正> Objective: To solve the problem of parameter estimate in the regression analysis of non-random sample.Methods: Calculating residuals according to the regression function based on original data. Modifying residuals andcorrecting them with mean. Adding mean-corrected residuals on original response and bootstrapping them to get 1000samples. Fitting regression functions of 1000 resampling samples and calculating the 2.5th percentile and 97.5th percen-tile of corresponding coefficient. Results: The interval estimates deriving from boostrap method had more statistical0|35|0Updated：2026-03-12
- Abstract：<正> Objective: It has been recommended that age-related centile curves of anthropometric indexes （such asheigh, weight） be used rountinly to evaluate the growth and development statues in children. Presented here is theage-related height centile curves for children aged 0-18 years in Shaanxi, China, 1995. Methods: Data of theShaanxi population were retrieved from The Third Nationwide Growth Survey to construct the age-related height centilecurves. The LMS method was used for curve fitting; all analyses were carried out on the basis of different sexes througha special program for LMS（λ-median-coefficient） method which was devised by Cole TJ. Results: Centile curves for0|26|0Updated：2026-03-12
- Abstract：<正> Using transesophageal echo-Doppler, it is demonstrated that preoperative acute hypervolemic hemodilution（HHD） with 6% hydroxyethel starch（HES） significantly increased in intravascular volume, DO2, CO2, SV and CI,markedly decreased in SVR and remained stable in MAP and HR, which indicates HES infused at 20 ml·kg-1 is safein patient without cardiac disease.0|9|0Updated：2026-03-12
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