Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smooth muscle cells

栾荣华; 贾国良; 李伟; 贾战生; 连建奇

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Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smooth muscle cells

Updated：2026-03-12

- Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smooth muscle cells
- Military Medical Research Issue 2, Pages: 90-94(2003)
- Affiliations：
  
  1. Department of Cardiovasology
  2. Xijing Hospital
  3. Fourth Military Medical University
  4. ,Xi’an,710038
  5. Department of Infectious Diseases
  6. Tangdu Hospital
- Author bio：
- Funds：
- DOI：
  CLC： R341
- Published：2003
- Accepted：
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[1]栾荣华,贾国良,李伟,贾战生,连建奇.Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smooth muscle cells[J].Journal of Medical Colleges of PLA,2003(02):90-94.
DOI：

[1]栾荣华,贾国良,李伟,贾战生,连建奇.Effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on proliferation of vascular smooth muscle cells[J].Journal of Medical Colleges of PLA,2003(02):90-94. DOI：

摘要

Abstract

<正> Ojective: To investigate the effect of hammerhead ribozyme that specifically cleaves c-myc mRNA on theproliferation of vascular smooth muscle cells （VSMCs）. Methods: Based on the computer analysis of the secondarystructure of c-myc mRNA

nt 2029 in rat c-myc oncogene was selected as a cleaving site for hammerhead ribozyme andthe ribozyme was designed. With automatic DNA synthesizer

the two complementary DNA strands of the ribozyme weresynthesized. The ribozyme gene was cloned into pGEM3Zf（+）vector and subcloned into eukaryotic expression pcD-NA3 vector. The recombinant pcDNA-Rz was transfected into the cultured rat VSMCs by lipofectAMINE mediated DNA

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