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Volume ， Issue 1 ， 2003
- Abstract：<正> Objective: To study cross-talk between p-opioid receptor and β-adrenoceptor through determination of the intracellular calcium （ [ Ca2+ ]i ） and cAMP responses in ventricular myocytes of rats subjected to chronic hypoxia for 4 weeks. Methods: Electrically-induced [ Ca2+ ]i transient was measured in single right ventricular myocytes isolated from hearts of chronically hypoxic rats and the age-matched normoxic rats, by using a spectrofluorometric method. Results: β-adrenoceptor stimulation with isoproterenol increased the electrically-induced [ Ca + ]i transient and cAMP in myocytes of normoxic rats. U50,488H, a selective β-opioid receptor agonist, at dose （1 βmol/L） which itself had no effect on the [Ca2 + ]i transient and cAMP, significantly inhibited the effect of isoproterenol. This inhibition was completely abolished in the presence of nor-BNI, a selective K-opioid receptor antagonist. In the ventricular myocytes of chronically hypoxic rats, the inhibition of U50,488H on the increased [ Ca ]i tra0|16|1Updated：2026-03-12
- Abstract：<正> Objective: To observe the series of pathological changes during the development of gastric adenocarcinoma in ulcerative rats induced by N-methyl-N’-nitro-N-nitrosoguanidine （MNNG） , and the expression profile of related on-cogenic protein. Methods: MNNG was administered in rats with ulcers due to acetic acid treatment to induce gastric cancer, and the protein expressions of ras and c-erbB2 genes in the ulcer were examined immunohistochemically along with pathological examination. Results: The incidence of gastric adenocarcinoma in the model group reaches 40% （6/ 15） , while none of the rats developed cancer in the control group with ulcers. Positive expressions of the proteins of p21ras and c-erbB2 were observed in the tissues undergoing canceration in the 6 rats of model group, but were not observed in the 5 control rats; p53 protein expression, however, failed to be detected in both groups. Conclusion: A new animal model of gastric cancer has been established in rats with gastric ulcer after MNNG trea0|30|1Updated：2026-03-12
- Abstract：<正> Objective: To examine the role of recombinant adenovirus vector containing CTLA4Ig gene（ Ad-CTLA4Ig） in the treatment of induced allergic rhinitis in mice. Methods: Allergic rhinitis was induced by sensitizing and challenging with ovalbumin （ OVA）. Ad-CTLA4Ig was intraperitoneally injected 30 min before OVA challenge. Adenovirus vector without inserted CTLA4Ig cDNA served as the control. The symptoms and morphological changes of nasal mucosa of each group were observed, and the serum levels of IgE against OVA were detected with ELISA. Results: There were no obvious symptoms and pathological changes in Ad-CTLA4Ig treated group, in which the serum OVA-specific IgE levels were significantly lower than that in control groups （P < 0. 05）. Conclusion: Ad-CTLA4Ig prevents and treats allergic rhinitis of mice, implying the possibility of the usage of Ad-CTLA4Ig against allergic rhinitis in clinic in future.0|23|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate a possible role of apoptosis in the pathophysiologic mechanisms of PIH （ pregnancy-induced hypertension syndrome）. Methods: In this study, placental samples were obtained from 16 uncomplicated third-trimester pregnancies and from 16 cases of PIH. We used light microscopy, electron microscopy to identify apoptosis. Light microscopy was used to quantify their incidence of apoptosis. Electron microscopy was used to confirm the occurrence of apoptosis. Results: Apoptosis has been conclusively demonstrated within human third-trimester placental tissue. Medians and interquartile ranges of normal placenta （n = 16） was 0. 12% （0. 08% -0. 19% ） ; Medians and interquartile ranges of PIH group （n = 16） was 0. 37% （0. 15% -0.49% ）. Compared to normal placentas, the incidence of apoptosis was higher in placentas from gestations complicated by PIH （ P < 0. 05 , T’-test）. Conclusion: Placental apoptosis increases significantly in PIH, and it may play a role in the pathophysiologic mechanisms0|7|0Updated：2026-03-12
- Abstract：<正> Objective: To further explore the mechanism of congenital pyrimidine 5’-nucleotidase I deficiency. Methods; The samples were collected from the family members of a patient with P5’N- I deficiency. The enzyme activities were measured by UMP method and the enzyme proteins were quantified by ELISA while the morphology of peripheral blood cells was observed. Results: The enzyme contents reduced as their enzyme activities decreased in the family especially in four members. There was a significant positive correlation（r =0. 955） between the activity and the content of P 5’N- I . The count of the stippling cell was varied in the family. Conclusion.- One of the reasons for congenital P5’ N- I deficiency might be the deficiency in the enzyme content. The morphology of peripheral blood erythrocyte may be an assistant diagnotic index. The P5’N- I activities and contents were measured simultaneously may be a effective method in clinic diagnosis.0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the possibility of the transfection of MyoD gene induced bone marrow mesenchymal stem cells （ MSCs） to differentiate into myoblasts in vitro. Methods: The eukaryotic expression plasmid vector pIRES2-EGFP-MyoD was transfected into MSCs with lipotransfection method, and the positive cells were selected by G418; The expression of MyoD was detected in the transfected MSCs with RT-PCR and the amplified, purified product was identified by sequencing; The reporter gene enhanced green fluorescence protein （ EFGP） was observed in the transfected cells under a fluorescent and a laser confocal microscopes; Immunohistochemical methods was used to examine the expressions of MyoD, myogenin, myosin, myoglobin and desmin in the differentiated cells. The ultrastructure changes of the cells before and after transfection were observed with electron microscopy. Results: The expression of MyoD was detected in the transfected MSCs with RT-PCR and the amplified, purified product was as same in sequence a0|47|1Updated：2026-03-12
- Abstract：<正> Objective: To study the effects of quercetin （QU） on matrix metallo-proteinases （MMPs） , the tissue inhibitor of matalloproteinase-1 （TIMP-1） , procollagen I and 2 proteoglycans （decorin and biglycan） mRNA expression in cultured rat hepatic stellate cell line HSC-T6 cells. Methods: Cells were treated with different concentrations of QU （12. 5, 25, 50 μmol/L） or drug solvent （0. 1 % Me2SO） for 24 h. mRNA expression was determined by reverse transcription polymerase chain reaction （RT-PCR）. Results: QU （12.5 - 50 μmol/L） enhanced collagenase （rat MMP-13） and membrane typel-MMP （MMP-14） mRNA expression, decreased procollagen I mRNA expression in a concentration-dependent manner, but did not affect gelatinase-A （MMP-2） , TIMP-1, decorin and biglycan expression. Conclusion: QU may decrease matrix deposition and increase matrix degradation, which might be beneficial to liver fibrosis.0|19|1Updated：2026-03-12
- Abstract：<正> Objective: To screen a triple helix-forming oligodeoxyribonucleotide （TFO） that can bind HBV core promoter at target site with high affinity and specificity, and to observe the ability of manganese porphyrin modified TFO to combine and cleave HBV DNA. Methods: Similar homopurine domain （1 734 - 1 754） in HBV core promoter was selected as target sequence. Several corresponding TFOs were synthesized. The affinities and specificities of TFOs binding target sequence were tested with electrophoretic mobility shift and DNase I footprinting assays. The selected best TFO was modified with manganese porphyrin and acridine. The ability of the TFO derivative to cleave HBV DNA was observed with cleavage experiment. Results: Under the condition of 371 and pH 7. 4, the TFO consisting of cytidylate and thymidylate （CT-TFO） and the parallel TFO consisting of guanylate and thymidylate （GT-TFOp） bound the target sequence weakly with Kd values much more than 10 -6 mol/L. The affinities of anti-parallel GT-TFO （ GT-TFOap）0|7|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the clinical value of QT dispersion （QTd） and the effects of 6-minute walk test （6-MWT） mimicking the patients’daily activities on QTd in patients with congestive heart failure （CHF）. Methods: Twenty-eight CHF patients and 22 normal subjects participated these study, who all completed 6-MWT without developing severe arrhythmias. Before and after 6-MWT, standardized 12-lead surface ECGs were obtained to measure QTd and corrected QTd （QTcd）. Results: Both before and after 6-MWT, the QTd and QTcd in CHF patients were longer than those in the controls （ P < 0. 001） , and QTd and QTcd after 6-MWT were significantly shorter than those before 6-MWT in CHF patients （ P = 0. 007 , and 0. 018）. There was no significant difference in the measurement in the control group. Conclusion: QTd and QTcd are longer in CHF patients than in normal subjects. Moderate exercise may improve the inhomogeneity of ventricular repolarization dispersion in CHF patients.0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To assess the efficacy and side effects of cyclophosphamide on refractory myasthenic crisis. Methods: Five patients of myasthenic crisis refractory to usual comprehensive treatment, entered an open additional study with cyclophosphamide 200 mg VD q. d or 400 mg VD q. o. d with 6-10 g of total dosage. The patients were followed up for 1-8 years. Results: All the 5 patients were effectively treated with obvious remission in 3 and improvement in 2. Two patients have returned to partial work. The side effects were tolerable. Conclusion: The present clinical trial showed that cyclophosphamide was effective, particularly in a long term as an additional therapy for treating MG patients with refractory crisis of myasthenia gravis.0|18|0Updated：2026-03-12
- Abstract：<正> Objective: To report two recent cases of pituitary adenoma associated with Toxoplasma gondii （T. Gondii） infection. Methods: Histological changes were observed in H & E and PAS staining sections microscopically. Immuno-histochemistry was performed to classify the pituitary tumors and to confirm the diagnosis of T. gondii. Results; The cases were 43- and 19-year-old females, in which the latter one was a recurring case, and radiology examination showed that tumors existed in sellar region. Microscopically, the tumors consisted of small homogenous polygonal or round cells with abundant eosinophilic granular cytoplasm. Immunohistochemistry revealed they were prolactin-producing adenomas. Interestingly, we found toxoplasma infection in the tumor tissues, being confirmed by T. gondii sepicific antibody immunohistochemistry. Conclusion: The association of pituitary adenoma with toxoplasma raises the possibility that T. gondii may be involved in the development of certain cases of pituitary adenoma.0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To evaluate penetration capacity of human sperm preserved in electrolyte-free （EF） solution at 4 ℃. Methods: The motility, acrosomal status penetration rate and fertility index of human sperm were assessed before and after cold-preservation in EF solution, respectively. Results: The motility of human sperm cold-preserved in EF solution for 1 week was significantly higher than that of human sperm cold-preserved in modified human tubal fluid （ mHTF） （43. 4% ± 7. 9% vs 9. 5% ±2. 5% , P <0. 01 ）. Although acrosomal status of human sperm cold-preserved in the EF solution before reinitiation was not different from those of the fresh sperm （ capacitated sperm; 7. 6% ± 1. 8% vs6.4±1.8%; acrosome-reacted sperm; 3.0% ±1.7% vs2.4±1.1%, P>0.05）, the percentage of capacitated and acrosome-reacted sperm in the EF solution significantly increased after reinitiation （capacitated sperm; 16. 0% ± 2.3%vs7.6±1.8%, acrosome-reacted sperm: 9. 4% ±2.1% vs 3. 0% ±1.7%, P<0.01）. The penetration rate and fe0|20|0Updated：2026-03-12
- Abstract：<正> AIM:To explore the more accurate lung sounds auscultation technology in high battlefield noise environment. METHODS: In this study, we restrain high background noise using a new method-adaptive noise canceling based on independent component analysis （ ANC-ICA） , the method, by incorporating both second-order and higher-order statistics can remove noise components of the primary input signal based on statistical independence. RESULTS: The algorithm retained the local feature of lung sounds while eliminating high background noise, and performed more effectively than the conventional LMS algorithm. CONCLUSION: This method can cancel high battlefield noise of lung sounds effectively thus can help diagnose lung disease more accurately.0|48|0Updated：2026-03-12
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