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Volume ， Issue 3 ， 2003
- Abstract：<正> To monitor the progression of lamivudine therapy by demonstrating HBV DNAs and RNAs in the serum of achronically infected patient. Viral nucleic acids were exracted from serum samples taken during a 14-week treatmentperiod and analyzed via competitive PCR and RT/PCR. The dynamic changes in the amount as well as in the structurescan monitor therapy process very well. Our study indicated the level of polyadenylated viral RNA can be a marker forexpression in general, and the appearance of replicative intermediates as a mirror of the mode of action of an antiviraldrug.0|7|0Updated：2026-03-12
- Abstract：<正> Objective: The whole mount preparations of digestive tract is an effective experimental way to study the ap-pearance and distribution of nerve plexus in digestive tract. Although myentric nerve plexus preparations technique wasreported very early. But we have done experiment over and over during our research work in order to improve this tradi-tional method and to meet the needs of our research work, we made some progresses in regular mount preparations aftermany experiments, which helped offer better situation in observing myentric nerve plexus. Methods: Five healthy maleadult Kunming mice （20-30g in weight） were used in this study. After intraperitoneal injection of muscle relaxant,with dislocation of cervical vertebra method, the abdominal cavity was exposed through abdominal median incision. Af-ter several steps of mount preparations the mucous layer and longitudinal muscle layer mount preparations wih myentricnerve plexus were stripped under anatomical microscope. Immunohistochemical staining was a0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To study the effect of glucocorticoid on the promoter of the pre-receptor glucocorticoid metabo-lizing enzyme 11β-hydroxysteroid dehydrogenase type 1（11β-HSD1）gene. Methods: The 1.2 kb length sequenceupstream to the transcription start site of the 11β-HSD1 gene was amplified with polymerase chain reaction （PCR） andthen was cloned into pBLCAT6 plasmid carrying chloramphenicol acetyltransferase （CAT） reporter gene. The plasmidpBLCAT6 carrying the promoter and reporter gene was used to transfect HeLa cells to study the regulation of 11β-HSD1gene expression by glucocorticoids in terms of reporter gene expression. Results: PCR showed that there was a com-plete alignment of the amplified sequence with the sequence 1.2 kb upstream to the transcription start site of 11β-HSD1gene. When cloned into pBLCAT6 plasmid carrying the reporter gene, this part of the promoter is functional in terms ofregulation of reporter gene expression upon transfection into HeLa cells. The synthetic glucocorticoid-dexa0|20|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the effect of arsenic sulfide （tetra-arsenic tetra-suffide As4S4; diarsenic trisulfideAs2S3）on tissue factor （TF） expression and procoagulant activity （PCA） of acute promyelocytic leukemia（APL） celllines （NB4 and MR2） and the basic mechanism of their role. Methods: NB4 and MR2 cells were respectively treatedwith As4S4, As2S3, As4S4 and Cyclohexamide（CHX）. PCA of the cells was detected using one-stage clotting assay.TF antigen was detected by ELISA. TF and PML/RARα fusion gene mRNA by semi-quantitive RT-PCR. The PCA andTF antigen of HL-60 and K562 cells were also examined. Results: The PCA and TF antigen level in NB4 and MR2cells were significantly higher than that in HL-60 and K562 cells. Both As4S4 and As2S3 can down-regulate the TF anti-gen, TF mRNA transcription and membrane PCA of NB4 and MR2 cells in vitro in a time-dependent manner. The roleof As4S4 was stronger than that of As2S3. Both As4S4 and As2S3 had no effect on PML/RARα fusion gene0|26|0Updated：2026-03-12
- Abstract：<正> Objective: To study the correlation between aquaporin-4 （AQP4） expression and diffusion-weighted ima-ging （DWI） in the process of ischemic brain edema for the molecular biologic mechanism of DWI. Methods: A total of34 Wistar rats were divided into 8 groups randomly: Non-operated group （n=4）, sham-operated group （n=6）, andoperated group, receiving right midddle cerebral artery occlusion （MCAO） for 15 and 30 min, and 1, 3, 6 and 24 h re-spectively （6 subgroups, n=4）. All groups were imaged with DWI and T2WI. The apparent diffusion coefficient（ADC）, relevant density （rd） and relevant area （rs） of hyperintensity of the lesions on DWI and T2WI were meas-ured. Relevant ADC （rADC）, relevant area of immunohistochemical staining for AQP4 （rS）, optical density of AQP4hybridization （α） were calculated. After that the animals were sacrificed and perfused at different time intervals, cor-relations between DWI, ADC, and AQP4 expression （rS,α） in ischemic tissue was made. Results: There was a sig-nificant correlat0|40|1Updated：2026-03-12
- Abstract：<正> Objective: To evaluate whether realgar could down-regulate human telomerase reverse transcriptase（hTERT） gene expression and telomerase activity in acute promyelocytic leukemia cell line-NB4 cells. Methods: Theexpression of hTERT-mRNA was analyzed by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR）. Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay （PCR-ELISA）.Flow cytometry using PI staining was applied to analyze the cell cycle and apoptosis. Results: Treatment of NB4 cellswith 155, 300, 600 μg/L realgar reduced telomerase activity significantly accompanying with decrease of hTERT-mR-NA and increasing cell apoptosis. G2/M phase arrest appeared when treated with realgar in 300, 600 μg/L. Condu-sion: It is suggested that telomerase activity of NB4 cells can be specifically inhibited by realgar through the down-regu-lation of hTERT gene expression. G2/M phase arrest and apoptosis by realgar in NB4 cells might be related to the re-ductio0|24|1Updated：2026-03-12
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Inhibiting effect of antisense hTRT on telomerase activity of human liver cancer cell line SMMC-7721
Abstract：<正> Objective: To induce changes in biological character of human liver cancer cell line SMMC-7721 by bloc-king the expression of telomerase genes hTRT and to explore its value in cancer gene therapy. Methods: The vehiclefor eukaryotic expression of antisense hTRT was constructed and then transfected into SMMC-7721 cells. The effects ofantisense hTRT gene on telomerase activity, cancer cell growth and malignant phenotypes were analyzed. Results: Theobtained transfectants that could express antisense hTRT gene stably showed marked decrease in telomerase activity;the shortening of telomere was obvious; cells presented contact growth inhibition; in nude mice transplantation, the rateof tumor induction dramatically decreased. Conclusion: Antisense hTRT gene expression can significantly inhibit te-lomerase activity of cancer cells and decrease malignant phenotypes in vitro and in vivo. Therefore, as a telomerase in-hibitor, antisense hTRT gene may be a new pathway for cancer therapy.0|19|0Updated：2026-03-12 - Abstract：<正> Objective: To improve the qualitative diagnosis of peripheral nerve sheath tumors by computed tomography（CT）. Methods: CT findings of 64 cases of pathologically confirmed nerve sheath tumors were compared with thepathological findings of the tumors. Results: Low density of the tumors shown in plain CT images was related to domi-nating reticular structure in the tumor as found pathologically. Tumors with intact capsule found by pathological findingswere shown with smooth margin in CT images. Inhomogeneous density and enhancement of the tumors in CT images wasrelated to tumor necrosis, liquefaction and cystic degeneration, and inhomogeneous enhancement also involved the re-ticular structure. Conclusion: Nerve sheath tumors are characterized by distribution along the nerves, lower densitythan that of muscles in plain CT images, and inhomogeneous enhancement in enhanced CT, which can help differenti-ate nerve sheath tumors from other soft tissue tumors. When nerve sheath tumors lack distinctive CT features,0|13|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the formation mechanism of benign biliary stricture. Methods: A model of trauma ofcommon bile duct was established in 28 dogs and then repaired. The anasomosis tissues were taken on the 1st week,3rd week and the 3rd month, 6th month respectively after operation and examined by using light microscopy and elec-tromicroscopy. Macrophage, TGF-β1, and α-SMA were studied immunohistochemically. Results: The mucosal epitheli-um of common bile duct restored poorly, chronic inflammation lasted for a long time, fibroblasts proliferated actively,extracellular matrix overdeposited; and myofibroblasts functioned actively and existed during the whole healing process.Immunohistochemical test showed a high expression of macrophage, TGF-β1, and α-SMA during healing process lastinga long duration. Macrophages were found in the lamina propria under mucosa, TGF-β1 in the granulation tissue, fibro-blasts and endothelial cells of blood vesssels, while α-SMA in the myofiroblasts and smooth muscle0|32|0Updated：2026-03-12
- Abstract：<正> Objective: To study the inhibition effects of estrogen on the production of corticotropin-releasing hormonein human placental cells. Methods: Primary cultured placental cells were treated by ICI182, 780, a complete ER an-tagonist, and Tamoxifen, an ERα-mixed agonist/antagonist and ERβ antagonist for 24 h. The supernatant was havestedfor the radioimmunoassay of CRH. Results: 17β-estradiol inhibited the secretion of corticotropin-releasing hormone inhuman placental （P<0.05）.ICI182, 780 stimulated the secretion of corticotropin-releasing hormone in human pla-cental（P<0.05）. Conclusion: Estrogen represses the synthesis and secretion of corticotropin-releasing hormone inhuman placental, which is possibly mediated by ERα.0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the clinical significance of magnetic resonance cholangiopancreatography（MRCP） utilizing half-Fourier acquisition single-shot fast spin-echo （HASTE） in the diagnosis of bile duct diseases.Methods: Forty-three patients with obstructive jaundice and 4 without were enrolled in this study. The underlying dis-eases included bile duct calculi（13 cases）, chronic cholangitis （14 cases） malignant tumors （18 cases） and congeni-tal biliary cysts （2 cases）. All patients underwent examinations with magnetic resonance imaging （MRI） and MRCP,and 39 were also examined with B-type ultrasonography, 33 with CT and 25 with ERCP and PTC. Three-dimensionalimage reconstruction was performed using volume-rendered technique （VRE） on the basis of the data obtained byMRCP. Results: The biliary calculi were displayed as circular filling defects in MRCP images, with the proximal endof dilated bile duct taking the form of the mouth of a cup. The bile duct of patients with chronic cholangitis showed dis-tal0|18|0Updated：2026-03-12
- Abstract：<正> ED50 of Pure Swainsonine （SW） to EMT6 cell strain is 1.2（0.8-1.6）mg/L and the dose of entire lethalis 6.4 mg/L with MTT method. After ig crude extraction of SW to S180 sareoma rooted in mouse and EMT6 model micewith breast carcinoma for 12 d at dose of 16 mg/kg, the inhibitory rates of weight amounts to 49.7% and 49.4% re-spectively （P<0.01）.Given crude extraction of SW at 4 mg/kg combined with irradiation at 5 Gy60Co-γ, the inhibi-tory rate of weight of S180 amount to 64.5% （P<0.01）.It shows that SW has obviously antineoplastic.0|46|8Updated：2026-03-12
- Abstract：<正> It was confirmed that sodium ferulate （SF） could significantly improve neurologic function deficit, reducecerebral infarct volume at 24 h after reperfusion, and weakened postsynaptic density-95 （PSD-95）activation in ische-mic area reacting to ischemia after transient middle cerebral artery occlusion （MCAO） by Western immunoblot analy-ses.0|20|0Updated：2026-03-12
- Abstract：<正> To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepaticVX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by usinga non-traumatic vascular clamp to block the branches distributing in the left-middle lobe of the hepatic artery for 60min, and subsequently the clamp was removed and the reperfusion injury of hepatic neoplasm occurred. At differenttime-points, the normal and hepatic neoplasm tissues of the animal models were taken out to detect the superoxide dis-mutase （SOD） and malondialdehyde （MDA） respectively. The results show that the products and injurious effects ofoxygen free radical （OFR） of the neoplasm tissues are more serious than those of the normal hepatic tissues.0|11|2Updated：2026-03-12
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