Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells
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Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells
Military Medical ResearchIssue 2, Pages: 73-76(2003)
Affiliations:
1. Department of Biochemistry & Molecular Biology
2. Third Military Medical University
3. ,Chengdu,610041
4. Department of Hematology
5. Xinqiao Hospital
6. West China University of Medical Science
Author bio:
Funds:
DOI:
CLC:R346
Published:2003
Accepted:
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[1]江渝,刘红,彭家和,叶治家,何凤田,董燕麟,刘秉文.Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells[J].Journal of Medical Colleges of PLA,2003(02):73-76.
DOI:
[1]江渝,刘红,彭家和,叶治家,何凤田,董燕麟,刘秉文.Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells[J].Journal of Medical Colleges of PLA,2003(02):73-76.DOI:
Oxidative modification of high density lipoprotein induced by cultured human arterial smooth muscle cells
摘要
Abstract
<正> Objective: To observe the oxidative modification of high density lipoprotein (HDL) induced by culturedhuman arterial smooth muscle cells (SMCs). Methods: HDL cocultured with SMCs at 37℃ in 48 h was subjected
and native HDL (N-HDL) served as control. Oxidative modification of HDL was identified by using agarose gel electro-phoresis. Absorbances of conjugated diene (CD) and lipid hydroperoxide (LOOH) were measured with ultravioletspectrophotometry at 234 and 560 nm respectively
and fluorescence intensity of thiobarbuturic acid reaction substance
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