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Volume ， Issue 4 ， 2003
- Abstract：<正> Objective: To study the effect of KATp channel opener and its possible mechanism on the sinoatrial node cells of neonatal rats which were cultured under simulated ischemia-reperfusion. Methods: Freshly isolated sinoatrial node （SAN） cells of neonatal rats were purified and cultured for 2 d, and then they were randomly divided into the control, simulated ischemia-reperfusion group （I/R group） , group intervened with KATp channel opener pinacidil （P + I/R group）, KATP Channel blocking agent 5-HD （5-HD + I/R group） , and group with the 2 agents at same time （5-HD + P + I/R group） . The survival rate of cells was measured by flow cytometry and the content of intracellular calcium in the cells of each group was detected with laser confocal microscopy. Results: ① The survival rate of SAN cells in I/R group [ （51. 79±6. 28）% ] was remarkably significantly lower than in control [ （95. 08±10. 48）% ] （P < 0.001）, and very significantly lower than in P + I/R group [ （63. 77±5. 35） % ] （P<0.01）, however, those0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To study the cause and mechanism of transplantation vasculopathy which characterized by accelerated graft arteriosclerosis （AGA） , we established a mouse aorta graft model. Methods: A segment of thoracic aortas of B10. A （2R） mice were transplanted to C57BL/10 mice abdominal aorta by end to side anastomoses. The different time point collected grafts were analyzed by morphological, histochemical and electro microscopic methods. Results: Rejection was manifested as a concentric progressive destruction of the smooth muscle cells. In contrast, the endothelial inflammation and subsequent neointimal proliferation characteristic of AGA was localized to the regions of turbulent flow, i. e. the junction of the graft with the recipient aorta. Conclusion: This model separates the processes of rejection and neointimal formation which usually manifested together in the lesion of AGA, elucidate that different mechanisms control vascular rejection and neointimal formation in chronic rejection.0|5|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the potential of superparamagnetic iron oxide particles （ SPIO） in MR imaging for the differentiation between hyperplastic and metastatic lymph node. Methods: Animal models of malignant lymph node metastasis were established in 6 New-Zealand rabbits by a unilateral intra-muscular injection of VX2 carcinoma cells, and models of hyperplastic lymph nodes were induced in another 6 rabbits by a unilateral intra-muscular injection of egg yolk emulsion. MR images of the lymph nodes were obtained before and 12 h after interstitial injection of SPIO. Image results were analyzed and compared with pathological findings. Results: On unenhanced images, the signal intensity of hyperplastic and metastatic lymph nodes did not differ significantly. After administration of SPIO, the signal intensity of both hyperplastic and metastatic lymph nodes remained unchanged on T1-weighted SE images. On T2-weighted SE images , the signal intensity of hyperplastic lymph nodes decreased heterogeneously, whi0|35|0Updated：2026-03-12
- Abstract：<正> Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIPI gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-viral vector GE7 gene delivery system was constructed. The malignant human glioma cell line U251MG was transfected in vitro with β-galactosidase gene （ reporter gene） and p21WAF-1/CIPI gee （therapeutic gene） using the GE7 system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21WAF-1/ CIPI gene in transfected U251MG cell was examined by immunohistochemis-try staining. Results: The highest transfer rate of exogenous gene was 70% . After transfection with p21WAF-1/CIPI gene, the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently. FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the0|13|0Updated：2026-03-12
- Abstract：<正> Objective: To study the memory biomechanical character of anatomic distal radius Nitinol memory connector （ DRMC） in treating distal radius fracture. Methods: Establishing three dimensional model and finite element analysis, we calculated the stress in and around the fracture faces when distal radius fracture was fixated with DRMC. Results: Axial holding stress produced by holding part of DRMC on distal radius was 14. 66 MPa. The maximum stress of holding part was 40-70 MPa, the minimum stress was 3-7 MPa, and the stress of compression part was 20 - 40 MPa. Conclusion: The distribution of stress produced by DRMC around the fracture line is reasonable, and axial holding stress can help stabilize fracture during earlier period. The existence of longitudal compression and memory effect can transfer fixated disused section into developed section and enhance fracture healing.0|18|1Updated：2026-03-12
- Abstract：<正> Objective: Morphine concentration measured in postmortem tissues may or may not reflect antemortem concentration. We measured levels of morphine in autopsied tissues to determine whether morphine distribution in morphine-dependent rats is altered after death. Methods: Solid-phase extraction was used to extract morphine from the samples, and morphine levels were measured at 0 - 96 h postmortem using gas chromatography. Results: The study of the morphine dependent rats showed a significant （P < 0. 05 ） increase of morphine concentration in postmortem cardiac blood, liver tissues and kidneys tissues. A significant increase was also observed at 72 h and 96 h postmortem in the brain, while morphine levels in cardiac tissues only increased at 24 h and 96 h postmortem. These changes were associated with an observed pH rapid decrease: pH of cardiac blood dropped from 7. 36±0. 15 to 6. 86±0. 09 （P < 0. 01） , pH of liver tissues from 6.98±0.04 to 6. 34±0.03 （P < 0.05）. Conclusion: The postmortem regional dist0|14|0Updated：2026-03-12
- Abstract：<正> Objective: To establish a stable reduced-size hepatic transplantation model in rats. Methods: Liver transplantation was performed in accordance with Kamada techniques. Many modifications were made including: Surgical manipulative innovation, adjustment of pre-operative drug administration and removed liver volume. Results: Forty-two rats underwent reduced-size hepatic transplantation; of them 33（84. 6% ） survived more than 1 week. The causes of postoperative death were peritonitis, inferior vena cava thrombus and unknown complications. Conclusion: Manipulative innovation and proper drug administration can improve the survival rate of rats apparently. Grafts regeneration can be triggered by the removal of left lateral segment and caudate lobe.0|5|4Updated：2026-03-12
- Abstract：<正> Objective: To identify the differential gene expression profiles between the normal and aspermia human testes utilizing cDNA microarray. Methods: cDNA probes were prepared by labeling mRNA of aspermia testes tissues with Cy5-dUTP and mRNA of normal testes tissues with Cy3-dUTP respectively through reverse transcription. The mixed cDNA probes were then hybridized with 4096 cDNA arrays （4096 unique human cDNA sequences） , and the fluorescent signals were scanned by ScanArray 3000 scanner （General Scanning, Inc. ）. The values of Cy5-dUTP and Cy3-dUTP on each spot were analyzed and calculated by ImaGene 3. 0 software （ BioDiscovery, Inc. ）. Differentially expressed genes were screened according to the criterion that the absolute value of natural logarithm of the ratio of Cy5-dUTP to Cy3-dUTP was greater-than 2. 0 or less-than 0. 5. A randomly chosen gene RAP1A was studied by in situ hybridization to evaluate the accuracy of the results. Results: 623 differential expressed genes related to aspermia were foun0|10|1Updated：2026-03-12
- Abstract：<正> Objective: To observe the inhibition of intracranial glioma tumorigenesis by vascular endothelial growth factor （VEGF） antisense oligodeoxynucleotide （ODN） in rats. Methods: Totally 20 μ1 Hank’s liquid containing 1×106 C6 glioma cells was seeded into rat right caudate putaraen in high-flow microinfusion with stereotactic technique. VEGF antisense ODN was simultaneously used with glioma cell. Each rat of the treated group Ⅰ and the treated group Ⅱ was treated with 1 000 μmol/L VEGF antisense ODN. Each rat of the treated group Ⅲ and the treated group Ⅳ was treated with 2 000 μmol/L VEGF antisense ODN. The experimental periods of the treated group Ⅰ , the treated group Ⅲ and the control group Ⅰ were 2 weeks, those of the treated group Ⅱ , the treated group Ⅳ and the control group Ⅱ were 3 weeks. Before sacrifice, MRI was performed on each rat. Tumor magnitude and pathologic examination were detected after samples were dissected. Results: The survival state of all treated rats was better, and0|4|0Updated：2026-03-12
- Abstract：<正> Objective: To prepare six quinazoline schiff bases by six steps of chemistry organic synthesis and test their inhibitory effect on hepatomacellular carcinoma cells HHCC and mammary cancer cell Bcap-37, furthmore, to compare their antitumor activities on these two kinds of cells. Methods: 2-Amino-5-nitro-benzylcarbonitrile was the initial material, and it was under the reaction of hydrolysis, ring-closing, halogenation, addition, reduction and substitution in turn to get the six quinazoline schiff bases, MTT method was adopted to compare their anticancer activities against the two cancer cells. Result and Conclusion: Six 6-imine-4-halo substituted anilinoquinozolines were prepared. The anti-cancer activities against both HHCC and Bcap-37were found, furthermore, they have more potency that on HHCC than on Bcap-37. In the six compounds, the schiff base Ⅵ is the most potent compound.0|31|1Updated：2026-03-12
- Abstract：<正> Objective: To investigate the therapeutic potency of recombinant human Granulocyte-Macrophage Colony-Stimulating Factor （GM-CSF） in a rabbit myocardial infarction model. Methods: A myocardial infarction was created by the ligation of the major ventricular branch of the left coronary artery in rabbits. After myocardial infarction, the animals were randomly assigned to GM-CSF treatment group, untreated groups and sham-operated group. The rabbits of the treated group were injected into GM-CSF by subcutaneous administration, 10 μg/kg/day, once a day for 5 days. The untreated and sham-operated group received a equal saline in the same manner as treated group. Six weeks later echocardiography and haemodynamic assessment were undertaken to assesse cardiac function. The size of the infarct region of the heart were also studied. Results: The untreated group exhibited significant higher left ventricle end-diastolic pressure, higher central venous pressure, and with significant lower mean blood pressure, lower pe0|25|4Updated：2026-03-12
- Abstract：<正> Objective: To investigate the relationship between estrogen receptor （ER） gene and the clinical indexes associated with coronary heart disease （ CHD）. Methods: By means of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） , we analyzed ER gene polymorphism in 84 CHD patients and 61 healthy subjects and non-CHD inpatients. The clinical indexes associated with CHD were analyzed in relation to the three ER genotypes. Results: There were significant differences in the incidence of hypertension （58.62% ）, fibrinogen （Fib） concentration （3. 5±0. 8 g/L） , body mass index （BMI, 25. 1±3. 2） , HDL-C concentration （1.0±0. 2 mmol/L） between PP genotype group and other genotype groups （P <0. 05）. Conclusion: ER gene polymorphism may affect ER-mediated cardiovascular protective effect by modulating the expression of ER.0|13|1Updated：2026-03-12
- Abstract：<正> Objective: To understand the role of urotensin Ⅱ （ U Ⅱ ） and proadrenomedullin N-terminal 20 peptide （ PAMP） , a fragment of proadrenomedullin （ proADM ） possessing biological activity, in the pathophysiological process of congestive heart failure （CHF） by observing the variation of their plasma levels and exploring their interrelations. Methods: Plasma U Ⅱ and PAMP levels were measured by radioimmunoassay in 52 patients with CHF and 14 healthy subjects. Left ventricular ejection fraction （LVEF） and the ratio of E/A were determined by echocardiography. Results: The plasma U Ⅱ level was significantly lower in patients with CHF than the healthy subjects （1.5±1.0 pg/ml vs 4. 3±1.2 pg/ml, P < 0.05）, while plasma PAMP level was significantly higher in the former group （30. 6±5. 8 pg/ml vs 21.0±6.6 pg/ml P < 0. 05 ）. The levels of U Ⅱ and PAMP were parallel with the severity of CHF, and significant correlation of plasma levels of U Ⅱ with LVEF （r=0.530, P=0.000） and the ratio of E/A (r=0.618, P = 0.0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To acquire a ribozyme against the E6 gene of human papillomaviruses type 16 （ HPV16E6） and investigate its effects on the phenotypes and gene expression of cervical cancer cell line. Methods: Anti-HPV16E6 ribozyme （ HRz） was designed by computer programs and its activity identified by cleavage experiment in vitro before its transfection via lipofectin into CaSKi cells with the empty eucaryotic expression plasmid transfection of the cells also performed, the resultant cells designated as CaSKi-R, CaSKi-P respectively. The morphology and the soft agar forming ability were studied in CaSKi cells and the transfected cells, and the expression of E6, proliferating cell nuclear antigen （ PCNA） and C-erbB-2 genes assayed by flow cytometry. The tumorgenicity of each cell line was evaluated in nude mice receiving inoculations of CaSKi, CaSKi-R and CaSKi-P cells separately, while in one group, both CaSKi and CaS-Ki-R cells were inoculated on different sides of the mice. Results: HRz was able to cleave H0|15|0Updated：2026-03-12
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