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Volume ， Issue 5 ， 2003
- Abstract：<正> Objective: To evaluate the therapeutic effects and mechanisms of Qidan granule in blemycinA5-induced pulmonary interstitial fibrosis （PIF）in rats. Methods: PIF models were established by blemycinA5-induced in rats. They were treated by Qidan granule and Hydrocortisone respectively. The pathological changes and collagen protein disposition were observed, and the expression of TGF-β, TNF-α proteins were measured by immunohistochemical technique . Results: The pulmonary alveolitis and fibrosis were alleviated remarkably in Qidan granule group compared with those in the model control group and hydrocortisone group （P <0. 01）. The expression of TGF-β and TNF-α protein were higher in Qidan granule group than those in normal group , and were significantly less than those in the model control group and in hydrocortisone group （P < 0. 01）. Conclusion: Qidan granule would ameliorate the pulmonary alveolitis and fibrosis. TGF-β and TNF-α might play an important role in the development of alveolitis and fibro0|18|0Updated：2026-03-12
- Abstract：<正> Objective: To evaluate the osteocompatibility of D, L-polylactic/hydroxyapatite/decalcifying bone matrix （PDLLA/HA/DBM） , and compare with PDLLA and DBM. Methods: Human primary osteoblasts isolated from the femoral head of patients were inoculated onto PDLLA/HA/DBM, PLA and DBM respectively. The proliferation rate and collagen Ⅰ expression were detected. The interface between biomaterial and osteoblasts was investigated with phase contrast microscopy and electron scanning microscopy. Results: Best proliferation rate was observed with the PDLLA/HA/DBM and followed by DBM and PLA, suggesting that PDLLA/HA/DBM satisfying most requirements for the cultivation of human osteoblasts. Scanning electron microscopy showed the morphology of osteoblasts was correlated with the proliferation data. The cells, well spread and flattened, were attached closely on the surface of biomaterial with an arched structure and had normal morphology. The extracellular collagenous matrixs covered the surface of biomaterial and pa0|14|0Updated：2026-03-12
- Abstract：<正> Objective: To study the relationship between nitric oxide synthase （ NOS） expression in human gastric mu-cosa and Helicobacter pylori （H. pylori） infection. Methods: Gastric mucosa samples were obtained from antrum of 33 patients received gastroendoscopy. H. pylori infection was confirmed by Giems staining and bacteria culture under mi-croaerophilic conditions. Expression of iNOS, eNOS and nitrotyrosine were detected by immunohistochemistry. Results: （1） The positive rate of H. pylori infection was 66. 7% （22/33） . （2） iNOS positive staining in inflammatory cells was detected in 77. 3% （17/22） of samples with H. pylori and 27. 3% （3/11） without H. pylori infection （P < 0.01）. （3） eNOS expression in inflammatory cells was found in 77. 3% （17/22） of samples with H. pylori and 18.2% （2/11） without H. pylori infection （ P < 0. 01） . （4） Nitrotyrosine expression in inflammatory cells was observed in 59. 1% （ 13/22） of samples with H. pylori and 54.5%（6/11） without H. pylori infection （P>0.05）. （5） Moderate a0|3|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the molecular regulation mechanism of carvedilol in attenuating the reversion back towards fetal energy metabolism during the development of cardiac hypertrophy induced by coarctation of abdominal aorta （ CAA） in male Wistar rats. Methods: Hemodynamic and ventricular remodeling parameters, free fatty acid content in the serum were measured in the experimental animals at 16 weeks after the surgical CAA, the rats receiving carvedilol intervention （CAR） after CAA, and those with sham operation （SH）. The expressions of muscle carnitine palmi-toyltransferase Ⅰ （M-CPT Ⅰ ） and medium chain acyl-CoA dehydrogenase （MCAD） mRNA in the cardiac myocytes from every group were studied with RT-PCR. Results: Significant left ventricular hypertrophy were observed in the rats 16 weeks after coarctation operation （P <0. 05） , together with significant free fatty acids accumulation and downregu-lation of M-CPT Ⅰ and MCAD mRNA （P <0. 05） in CAA group. Carvedilol at a dose of 30 mg/kg/d for 12 weeks i0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To study the role of macrophage inflammatory protein （ MIP） -2γ in myocarditis pathogenesis in BALB/c mice. Methods: The relationship between the progression of Coxsarckie virus B3 （ CVB3 ） viral myocarditis and experimental autoimmune myocarditis and MIP-2γ mRNA expression in mouse was studied by TaqMan real-time fluorescent quantitative RT-PCR. Results: MIP-2γ mRNA expression rose on 3 to 5 d after CVB3 infection, reached peak on 7 d, and returned to normal level until 14 d, which corresponded well with the disease course. The MIP-2γ mRNA expression level rose significantly on the day 18 d after immunization with porcine cardiac myosin, which was consistent with pathological examination. Conclusion: MIP-2γ may be involved in the pathogenesis of myocarditis.0|22|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the changes of the plasma level of MMP-9 （ Matrix Metalloproteinase-9, MMP-9） in the patients with abdominal aortic aneurysms （ AAAs） before and after the treatment, and evaluate the significance of MMP-9 in the pathogenesis of AAAs. Methods: Blood samples of 35 patients with AAAs and 10 patients with the arterial occlusive diseases （ AODs） , which enrolled into the Vascular Surgery Center of Colonge University Hospital from February to August of 2002, were collected before and one month after surgical repair or less-invasive endovascu-lar exclusion. The plasma concentrations of MMP-9 of all the collected samples were measured by means of enzyme-linked immunosorbent assay （ ELISA） , and compared between the two groups patients at different time point. Results: The mean plasma concentration of MMP-9 of AAAs was significantly higher than that of AODs prior to treatment [（90.3±9.1） ng/ml vs （23. 6±7. 3） ng/ml, P < 0. 05 ] , and no apparent difference was showed in the patients w0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate changes of Ca2+ activated potassium channels （ KCa） in autogenous vein grafts. Methods: Contraction of venous ring was measured by means of perfusion in vitro. The intimal rabbits proliferation of vascular and proliferation of cultured smooth muscle cells （ vascular smooth muscle cells, VSMCs） were observed by the means of computerised image analysis and MTT method respectively. Furthermore, whole cell mode of patch clamp was used to record KCa of VSMCs isolated from autogenous vein grafts. Results: One week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation （P < 0. 05 , n = 8 vs control） , and they was more enhanced 4 weeks after vein transplantation （ P < 0. 01 , n =8 vs control ）. TEA（ blocker of Ca2+ activated potassium channels） increased MTT A490 nm0|7|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the trends of incidence and mortality rates of stroke in Hanzhong rural population. Methods: Acting as the WHO MONICA project. Results: The incidence rate of stroke was 152. 9/100 000. There was decline trend in male（P <0. 05）. The mortality rate of stroke was 115.9/100 000. There was no significant decline trend during 18-year period （P < 0. 05）. The incidence and mortality rates of stroke of male were higher than those of female（P<0. 05）. The incidence and mortality rates were all increased with age（P <0. 01 ）. Conclusion: It must stick to the long- term prevention measures to decrease incidence rate, and improve the condition of medical treatment to reduce the mortality rate in rural population.0|12|0Updated：2026-03-12
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