In vitro effect of p2l<sup>WAF-1/CIP1</sup> gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system

陈永新; 许秀兰; 张光霁; 王韦; 金海英; 卢亦成; 朱诚; 顾健人

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In vitro effect of p2l^WAF-1/CIP1 gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system

Updated：2026-03-12

- In vitro effect of p2l^WAF-1/CIP1 gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system
- Military Medical Research Issue 4, Pages: 222-225(2003)
- Affiliations：
  
  1. Department of Neurosurgery
  2. Changzheng Hospital
  3. Second Military Medical University
  4. ,Shanghai,200032
  5. China Department of Neurosurgery
  6. No. 85 Hospital of Chinese PLA
  7. National Laboratory for Oncogenes and Related Genes
  8. Shanghai Cancer Institute
- Author bio：
- Funds：
- DOI：
  CLC： R73-3
- Published：2003
- Accepted：
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[1]陈永新,许秀兰,张光霁,王韦,金海英,卢亦成,朱诚,顾健人.In vitro effect of p2l~(WAF-1/CIP1) gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system[J].Journal of Medical Colleges of PLA,2003(04):222-225+250.
DOI：

[1]陈永新,许秀兰,张光霁,王韦,金海英,卢亦成,朱诚,顾健人.In vitro effect of p2l~(WAF-1/CIP1) gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system[J].Journal of Medical Colleges of PLA,2003(04):222-225+250. DOI：

摘要

Abstract

<正> Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIPI gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-viral vector GE7 gene delivery system was constructed. The malignant human glioma cell line U251MG was transfected in vitro with β-galactosidase gene （ reporter gene） and p21WAF-1/CIPI gee （therapeutic gene） using the GE7 system. By means of X-gal staining

MTS and FACS

the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21WAF-1/ CIPI gene in transfected U251MG cell was examined by immunohistochemis-try staining. Results: The highest transfer rate of exogenous gene was 70% . After transfection with p21WAF-1/CIPI gene

the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently. FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the

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In vitro effect of p2lWAF-1/CIP1 gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system

DOI：

摘要

Abstract

关键词

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references

In vitro effect of p2l^WAF-1/CIP1 gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system