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Volume ， Issue 3 ， 2002
- Abstract：<正> Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunocytochemistry was applied to detect antigens such as BrdU, Nestin and specific markers for mature neural cells. Results: Cell groups isolated from midbrain propagated in succession and shaped ’neuro-spheres’. Nestin antigen was expressed in the course of proliferation. After differentiation, epitopes of neuron , astrocyte and oligodendrocyte were all found in the neural progenitors-derived cells, the most majority were astrocyte-epitoped cells. Conclusion: Cell groups expressing Nestin isolated from midbrain of new-born rats have properties of propagating, multi-potency differentiating and self-renewal. It is demonstrated that the cell groups are stem-like neural progenitors in the central nervous system, which are qualified for cell therapy of diseases in central nervous system.0|10|0Updated：2026-03-12
- Abstract：<正> Objective: To establish a reformative detection system which has sound ability of providing information on molecular mutagenesis spectrum and the specificity of detection system of repackaged λ phage. Methods: LacZ gene, as mutational target gene and reporter gene, was applied into the detection system. The λ gt11 DNA treated with ENU （1-ethyl-1-nitrosourea） and 9-AA （9-aminoacridine） was repackaged in vitro. The packaged λ phage was then grown in E. coli Y1090 on a selective plate containing X-gel and IPTG. The survival and mutation frequencies were determined by counting the clear-plaque and blue-plaque, and the molecular mutation mechanism was studied by extracting and sequencing the LacZ gene of mutants. Results: The survival of repackaged λ phages treated with 9-AA and ENU apparently decreased in consistent dose-dependence. The mutation frequency of clear-plaque mutants showed a linear dose-related increase. The predominant mutations induced by 9-AA were ± 1 frameshift mutation, and 9-AA induc0|21|0Updated：2026-03-12
- Abstract：<正> Objective: To discuss the relationship between hypercholesterolemic disease and the functional and structural changes of Sphincter of Oddi （SO） by the study of effect of Cholesterol Liposome （CL） on structural and quantitative changes of SO cells. Methods: Rabbit SO was isolated for primary cell culture and subculture. After subcultured with different concentration of CL culture medium for 20 h, the structural and quantitative changes of SO cells were analyzed and detected by MTT-test, flow cytometer （FCM）, electronic microscope and electrophoresis technique respectively. Results: CL contributed a prominent stimulus to SO cells proliferation at middle concentration （<0. 5 - 0. 8 mg/ml）, which could be confirmed by FCM analysis which indicated the number of SO cells in S-phase increasing remarkably; however, high concentration of CL inhibited SO cells’ proliferation （>1. 0 mg/ml） and induced apoptosis of SO cells. Swelled mitochondria and dilated endoplasmic reticulum as well as disjoined and diminished0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin （FN） from cultured rat mesangial cells and its possible mechanism. Methods: （1） MCs seeded at a density of 1 × 104 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34（10-12 mol/l -10-8 mol/l） for 6 h, 12 h, 24 h and 48 h, control cells were treated with vehicle only. The FN levels （in the supernatant） were measured by ELISA assay. （2） MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and various concentrations of hPTH1-34（10-12 mol/l - 10-8 mol/l ）. Forty-eight hours later, FN were tested by ELISA. （4） MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and 10-8 mol/l hPTH1-34 for 6 h, 12 h, 24 h and 48 h and then FN were tested. Results: （1） hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10-8 mol/l （P<0. 01）. （2） Anti-TGF-β antibody inhibited the stimulation effect of hPTH1-34, on synthesis of FN in cultured rat mesangial cells0|8|0Updated：2026-03-12
- Abstract：<正> Objective: In order to get more information about the possible functions of Calbindin D-28K in the central nervous system of adult cat, the distribution of Calbindin D-28K in the central nervous system of adult cat was examined. Methods: Immunohistochemical staining techniques were used, and immunostained sections were observed under a light microscopy. Results: A high density of both immunoreactive perikarya and fibers were observed in the basal ganglia, amygdaloid complex, nucleus of the fields of Forel, subthalam-ic nucleus, paracentral nucleus, pulvinar nucleus, subthalamus, dorsal hypothalamic area, lateral hypothala-mic area, anterior hypothalamus, suprachiasmatic nucleus, superior colliculus, inferior colliculus, oculomotor nucleus, superior olivary complex, marginal nucleus of the brachium conjunctivum, vestibular nuclei, the spinal trigeminal nucleus, nucleus of the solitary tract, cuneate nucleus, inferior olivary complex, dorsal motor nucleus of the vagus nerve, the molecular layer of the cer0|21|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the possible mechanisms of growth regression of human androgen dependent prostate carcinoma cells caused by androgen withdrawal. Methods: After 24 h of treatment with 1 × 10-9 mol/L dihydrotestosterone （DHT）, the expression of phosphorylated ERK proteins and cell cycle regulation molecules including CDK2, CDK4, CDK6 and P27kip1 in human androgen dependent prostate carcinoma cell line LNCaP was measured by Western blot analysis 0 h, 8 h and 24 h of after androgen withdrawal. Human androgen independent prostate carcinoma cell line PC-3 was also examined as control. Results: Down-regulation of phosphorylated ERK, CDK2, CDK4 and CDK6 and up-regulation of P27kip1 were found initially in LNCaP cell line 8 h after androgen withdrawal. The levels of phosphorylated ERK and CDKs decreased continuously and reached the lowest after 24 h, while continuous elevation of P27kip1 was detected thereafter to 24 h. No expression change of phosphorylated ERK, CDKs and P27kip1 were detected in PC-3 cel0|27|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the effects of γ-irradiation on mitogen-activated protein kinases （MAPKs） and role of intracellular calcium in this event in intestinal epithelial cell line 6 （IEC-6 cells）. Methods: After cultured rat IIEC-6 cells with or without the pretreatment of intracellular Ca2+ chelator were exposed to Y-ir-radiation of 6 Gy, the total and phosphorylated MAPKs in the cells were determined with Western blotting and apoptosis was examined with flow cytometry. Activities of Extracellular signal-regulated protein kinase （ERK） and p38 MAPK were determined by using immuoprecipitation followed by Western blotting. Results: In response to γ-irradiation, phosphorylation of ERK was not significantly observed, while the levels of phosphorylated c-Jun NH2-terminal kinase （JNK） and p38 MAPK were increased in 30 min and reached the peak 2 h after exposure to 6 Gy γ-irradiation, though the cell viability was significantly lowered 12 h. On the other hand, no obvious changes were seen in the total protei0|22|0Updated：2026-03-12
- Abstract：<正> Objective: To study the effect of tacrolimus （Prograf, FK506） in preventing renal allograft rejection. Methods: The curative effect, therapy index, toxicity and side effects of FK506 were observed in 294 renal transplant recipients among whom 268 received FK506 24 h after the operation and the other 26 with cyclosporine （CsA） developed acute rejection after transplantation and were given FK506 to replace methyl-prednisolone （MP） when the latter did not result. All the patients were given oral mycophenolate mofetil （MMF, 1. 0 g/d） and meticorten （Pred, 30 mg/d） 24 h later after operation. Results: In the 268 recipients previously mentioned, the incidence of acute rejection was 10. 45%, glycometabolism disorder 9. 33%, nervous system disturbance 1. 59%, liver function abnormality 2. 99%, nephrotoxicity 1. 87%, gastrointestinal disorder 17. 5%, cytomegalovirus （CMV） viremia 2. 99%, and non-CMV pulmonary infection 1. 59% （4/268）, with 1 fatal case for cerebral hemorrhage with normal allograft function and a0|14|0Updated：2026-03-12
- Abstract：<正> Objective: To study whether stem cell factor （SCF） and gonadotropin work synergisticly in super-ovulation stimulation of an in-vitro fertilization and embryo transfer （IVF-ET） program. Methods: Total cycles of 30 IVF-ET patients with regular menstrual period were studied. The same superovulation regimen was employed. Enzyme-linked immunosorbent assay （ELISA） and radioimmunoassay （RIA） were used to determine the levels of SCF, follicular-stimulating hormone （FSH） and luteinizing hormone （LH） in follicu-lar fluid （FF） during oocyte pick-up （OPU） and in serum before and after superovulation. Results: FF-SCF levels were significantly higher in high and moderate reactive groups [（564±64） ng · L-1, （532±55） ng · L-1, respectively] than that in low reactive group [（352±78） ng · L-1, P<0. 01]; FF-FSH levels were significantly different in three groups [（23. 7±5. 7） U · L-1, （24. 5±8. 9） U · L-1, and （15. 4±4.1） U · L-1, respectively, P<0. 05]. FF-LH levels were lower in low reactive group (33. 0±7.0|12|0Updated：2026-03-12
- Abstract：<正> Objective: To explore whether successful valvuloplasty increases mitral valve reserve capacity in patients with mitral stenosis. Methods: Thirty-eight patients with pure rheumatic mitral stenosis underwent isoproterenol stress echocardiography before and after successful percutaneous balloon valvuloplasty. The mitral valve area （by direct planimetry of two-dimensional echocardiography）, mean transmitral pressure gradient （by continuous-wave Doppler echocardiography）, and cardiac output （by M-mode echocardiography） were measured at rest and under isoproterenol infusion to achieve heart rate of different stages. Results:Between the measurements before and after valvuloplasty, significant differences were observed in the mitral valve area （0. 91±0. 28 vs 1. 87±0. 23 cm2, P<0. 01）, mean transmitral pressure gradient （12. 5±6. 3 vs 3. 9±1. 9 mmHg, P<0. 01） and cardiac output （3. 93±1. 44 vs 4. 73±1. 01 L/min, P<0. 05） at rest. Before valvuloplasty, the mean transmitral pressure gradient increased signi0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To assess the clinical value of trans-abdominal ultrasonography for the diagnosis of achalasia. Methods: The subjects were divided into 3 groups, with 8 patients suffering from achalasia as the patient group, 10 normal individuals and 8 patients with cardiac carcinoma as controls. Real-time trans-abdominal ultrasonography （TAUS） was employed to investigate the lower esophagus and cardia of all subjects through water provocative test, and the sonographic findings were compared. After treated by administration of drugs, pneumatic balloon dilation or intrasphincteric injection of botulinum toxin, the 8 patients with achalasia were re-examined by TAUS for longitudinal comparison. Results: （1） The normal subjects had no dilation of cardia and lower esophagus before drinking water, but their cardias opened rapidly in accordance with the drinking action to allow water enter the stomach. （2） All the patients with achalasia were found to have dilation of lower esophagus before taking water. During a 10|9|0Updated：2026-03-12
- Abstract：<正> Objective: To evaluate the therapeutic effects of 0. 014" Choice PTTM wire in chronic total occlusion angioplasty. Methods: Balloon angioplasty was attempted in 25 arteries with chronic total occlusion, with the mean time of occlusion of 17±13 months （ranging from 2 to 84 months） and mean length of 14±6 mm （ranging from 5 to 25 mm）. The morphology of the lesions included bridging collaterals （4 cases）, calcification （3 cases） and major side branch at the lesion （4 cases） . Choice PT?wire was used electively in all the cases. Results: Lesion was crossed successfully in 92% （23/25） cases, without incidences of dissection of the coronary artery with subintimal entry. Balloon angioplasy and stenting （n = 21） were performed with good immediate angiograghic results. Acute myocardial infarction or death occurred in none of the patients. Conclusion Successful recanalization of chronic coronary total occlusions using Choice PTTM wire can be achieved with good safety.0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome （SIRS） and possible mechanism. Methods: Escherichia coli DNA （EC DNA） was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide （LPS） were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship （LD50 = 11. 51 mg/kg）, but CT DNA didn’t. The peak level of TNF-α was lower in EC DNA group than in LPS group （P<0. 05）, though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of s0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To treat humerus fracture with three dimensional pattern and finite element analysis, providing mechanical basis for treating humerus fracture. Methods: Humerus pattern was established based on the CT images, and calculation was done by ANSYS5. 6 software. Three dimensional ten-node tetrahedron unit was selected and were divided into 2 729 nodes, 49 041 units. Distribution and amount of axial compression of humerus were analyzed when clip angle was 30°, 45°, 90° between fracture face and axial line with fixed X, Y, Z directions. Results: The distribution of stress was greatly different between fracture face and non fracture face. Stress in fracture part was fairly concentrated with incomplete symmetric distribution around the center of fracture face; Greater stress distributed in the regions 10 mm from fracture face, which was 2-3 times that of other stress regions. Conclusion: Required load must be estimated under various conditions as to select the suitable internal fixation implants dur0|21|3Updated：2026-03-12
- Abstract：<正> Objective: To investigate the features of hepatic abscess by dynamic contrast-enhanced CT. Methods : CT films of 62 cases of hepatic abscesses were reviewed retrospectively. All the patients underwent both plain and enhanced CT scanning, and 23 of them received dynamic CT examination. Results: The dynamic CT appearance of hepatic abscesses was characterized by: ① In the early phase （30 s）, abscess membrane enhanced obviously and the liver parenchyma surrounding the abscesses enhanced transiently; ② In the late phase （60-90 s） , the enhancement patterns of the abscesses varied from the ring enhancement of the abscess membrane to the irregular enhancement of the abscess; ③ In the delayed phase, the enhancement of abscess diminished in which the hypodense ring enhanced and became isodense, or the abscess enhanced diffusely and became smaller, the colliquative necrosis area more obvious. Conclusion: Contrast enhanced CT should be used routinely in the evaluation of hepatic abscesses. The dynamic and dela0|4|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the relationship among the TNFRp55 and TNFRp75 expression in tumor cell lines in vitro, the suppression of nrhTNF on the tumor cell lines growth and the changing of NF-kB activity in the tumor cell lines. Methods:The expressions of TNFRs are studied by immunohistochemistry. The inhibition of nrhTNF to tumor cells was investigate by crystal violet assay. The changing of activity of NF-icB was evaluated by using immunohistochemistry and Western blot methods. Results: In tumor cell lines, positive rates of expression of TNFRp55 and TNFRp75 were 99% and 98. 8% respectively. There was no significant difference among various types of tumor cells （P>0. 05）. nrhTNF could inhibit the growth of SW480, SGC7901, 8910, SMMC7721 and BEL-7402 cell lines in which NF-icB translocated into cytonuclei slightly. However, nrhTNF has no effects on A549, PLA801, Hela, Ecal09 and GRC-1 cell lines in which NF-kB translocated into cytonuclei obviously. Conclusion: The low degree of activating of NF-kB m0|33|0Updated：2026-03-12
- Abstract：<正> Objective: To observe the effect of anciclovir （ACV） treatment on tumors induced by inoculation of TK gene-transfected human pulmonary adenocarcinoma A549 cells in nude mice. Methods: A recombinant plasmid containing TK gene was constructed and transfected into A549 cells by electroporation. The sensitivity of the transgenic cells （A549-TK） to ACV was examined by MTT assay in vitro and for in vivo observation, inoculation of A549-TK and A-549 cells into nude mice was separately performed to induce tumor growth, the response of which to ACV treatment was observed, and the tumor tissues were pathologically examined. Results: A recombinant plasmid containing TK gene was successfully constructed and transfected into A549 cells. The sensitivity of A549-TK cells to ACV was 43 times higher than that of A549 cells. The tumors induced by A549-TK cells showed no significant increase in size after ACV treatment （P>0. 05） , and light microscopy revealed local tissue necrosis, karyoklasis, and nuclei disappearance.0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the levels of serum and ascitic fluid soluble tumor necrosis factor receptor-p55 （sTNFR-p55） and understand their clinical implication in primary hepatocellular carcinoma （HCC） patients. Methods: Enzyme-linked immunosorbent assay （ELISA） was used to examine the levels of sTNFR-p55 in the serum and ascitic fluid in 25 HCC patients and 25 patients with liver cirrhosis （LC）. The test was also performed on the serum of 30 healthy subjects who served as control group. To assess the clinical effects of increased serum concentrations of sTNFR-p55, four parameters were analyzed by logistic regression. Results: Serum and ascitic fluid levels of sTNFR-p55 in HCC patients were significantly higher than those in LC patients and controls （P=0. 001）. No significant difference was found between serum sTNFR-p55 levels in the latter 2 groups （P = 0. 19）, and positive correlation between serum levels of sTNFR-p55 and that in ascitic fluid was noted in the 2 patient groups （r=1. 000, P<0. 001）. Level0|17|1Updated：2026-03-12
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