Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo

何祥梁; 何东华; 郭先健; 钱桂生; 黄桂君; 陈维忠; 李淑萍

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Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo

Updated：2026-03-12

- Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo
- Military Medical Research Issue 3, Pages: 227-231(2002)
- Affiliations：
  
  1. Department of Respiratory Diseases
  2. Zhujiang Hospital
  3. First Military Medical University
  4. ,Guangzhou,510120
  5. Da’an Gene Center
  6. College of Medical Sciences
  7. Sun Yat-sen University
  8. Institute of Respiratory Diseases of PLA
  9. Xinqiao Hospital
  10. Third Military Medical University
  11. Chongqin,400037
- Author bio：
- Funds：
- DOI：
  CLC： R734.2
- Published：2002
- Accepted：
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[1]何祥梁,何东华,郭先健,钱桂生,黄桂君,陈维忠,李淑萍.Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo[J].Journal of Medical Colleges of PLA,2002(03):227-231.
DOI：

[1]何祥梁,何东华,郭先健,钱桂生,黄桂君,陈维忠,李淑萍.Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo[J].Journal of Medical Colleges of PLA,2002(03):227-231. DOI：

摘要

Abstract

<正> Objective: To observe the effect of anciclovir （ACV） treatment on tumors induced by inoculation of TK gene-transfected human pulmonary adenocarcinoma A549 cells in nude mice. Methods: A recombinant plasmid containing TK gene was constructed and transfected into A549 cells by electroporation. The sensitivity of the transgenic cells （A549-TK） to ACV was examined by MTT assay in vitro and for in vivo observation

inoculation of A549-TK and A-549 cells into nude mice was separately performed to induce tumor growth

the response of which to ACV treatment was observed

and the tumor tissues were pathologically examined. Results: A recombinant plasmid containing TK gene was successfully constructed and transfected into A549 cells. The sensitivity of A549-TK cells to ACV was 43 times higher than that of A549 cells. The tumors induced by A549-TK cells showed no significant increase in size after ACV treatment （P>0. 05）

and light microscopy revealed local tissue necrosis

karyoklasis

and nuclei disappearance.

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