<正> Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin （FN） from cultured rat mesangial cells and its possible mechanism. Methods: （1） MCs seeded at a density of 1 × 104 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34（10-12 mol/l -10-8 mol/l） for 6 h

12 h

24 h and 48 h

control cells were treated with vehicle only. The FN levels （in the supernatant） were measured by ELISA assay. （2） MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and various concentrations of hPTH1-34（10-12 mol/l - 10-8 mol/l ）. Forty-eight hours later

FN were tested by ELISA. （4） MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and 10-8 mol/l hPTH1-34 for 6 h

12 h

24 h and 48 h and then FN were tested. Results: （1） hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10-8 mol/l （P<0. 01）. （2） Anti-TGF-β antibody inhibited the stimulation effect of hPTH1-34

on synthesis of FN in cultured rat mesangial cells