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Volume ， Issue 4 ， 2002
- Abstract：<正> Objective: To examine the role of p38 mitogen-activated protein kinase （MAPK） in NO production and iNOS expression in human endothelial cells stimulated by lipopolysaccharide （LPS）. Methods: The NO level in the supernatant of the cell culture media was measured with Griess method, expressions of iNOS protein and mRNA in vitro cultured endothelial cell line ECV304 were detected with immunofluorescence analysis and reverse transcriptase-PCR respectively. Immunokinase assay was employed to measure p38 MAPK activity. Results: Compared with the basal level of iNOS expression and NO production, the NO level and the expressions of iNOS mRNA and protein in the cells were increased after LPS stimulation. p38 MAPK activity in ECV304 cells exhibited a marked increase at 15 min after LPS stimulation, lasting for about 45 min before gradually decline. The iNOS protein and mRNA expressions induced by LPS stimulation was significantly inhibited by SB203580 [4-（4-fluorophenyl）-2-（4- methylsulfinylphenyl）-5-（4-pyridyl）0|28|1Updated：2026-03-12
- Abstract：<正> Objective: To establish a novel approach for quick and highly efficient verification of human gene imprinting. Methods: A pair of dye-labelled probes, 5’ nuclease assay was combined with RT-PCR to determine the genotype of a transcribed single nucleotide polymorphism （SNP） rs705（C>T） of a known imprinted gene, small nuclear ribonucleotide protein N （SNRPN）, on both genomic DNA and cDNA of human lym-phoblast cell lines. Results: Allele discrimination showed a clear monoallelic expression pattern of SNRPN, which was confirmed by RT-PCR based restriction fragment length polymorphism （RFLPs）. Pedigree analysis verified the paternal origin of expressed allele, which was in consistency with previous report. Conclusion: Transcribed SNP is an ideal marker for detecting gene imprinting by 5’ nuclease assay. This approach also may be used to discover differential allele expression of non-imprinted genes, finding out gene cis-acting functional polymorphism.0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the effect and mechanism of nordihydroguaiaretic acid （NDGA） on apop-tosis in human malignant glioma cell line SHG-44. Methods: Cell growth inhibition was measured with MTT assay. Cell apoptosis was observed with light and electron microscopy and TUNEL. Expression of bcl-2 gene was measured with immunohistochemistry, in situ hybridization and image analyses. Results: NDGA at the concentration of 100 μmol/L inhibited the proliferation of SHG-44 cells and induced apoptosis in a time-dependent manner. The expression of Bcl-2 protein in SHG-44 cells was decreased in the present of 100 μmol/L NDGA along with the duration of treatment in a negative correlation with the degree of cell apoptosis. The bcl-2 mRNA expressed in SHG-44 cells was reduced after treatment with 100 μmol/L NDGA, apparently consistent with the immunohistochemical results. Conclusion: NDGA can induce apoptosis of human malignant glioma cells probably by down-regulating expression of bcl-2 gene, though the exact0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the development and distribution of phosphate-activated glutaminase like immunoreactive （PAG-LI） neurons in the central pathway of trigeminal proprioception of the rat brainstem. Methods: The immunohistochemitry techniques were used. Results: （1） At embryonic day 17 （E17）, PAG-LI neurons were initially observed in the mesencephalic trigeminal nucleus （Vme）. All PAG-LI neurons were large round neurons with moderate immunostaining. The immunoreactivity grew intense and attained adult-like pattern at P10. （2） Not until postnatal day 10 （P10） did a few PAG-LI neurons appear in the area ventral to the motor trigeminal nucleus （AVM） and area dorsal to the superior olivery nucleus （ADO）, and not until P12 in the dorsomedial part of the subnucleus oralis of the spinal trigeminal nucleus （Vodm） and dorso-medial part of the principal sensory trigeminal nucleus （Vpdm）. As development proceeded, more and more neurons in them were immunostained, and some PAG-LI neurons were detected in the0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the mechanism of the degradation of human hair keratin （HHK） scaffold material implanted in damaged skeletal muscle tissues. Methods: Six New Zealand rabbits with HHK scaffold material implants （composed of 3 different types of HHK material with varied degradation speed） after mus-clectomy were divided into 3 groups （2 in each group） to observe the degradation of the material at 1, 3, 6 weeks after operation. Another rabbit without operation was used as the control group. The degradation of HHK was observed with light microscopy, histochemistry of ubiquitin and electron microscopy. Results: Light microscopy showed that human hair cuticles fell off from the HHK material and emerged, and the macrophagocytes and multinucleate giant cells were attached onto the surface of the material, which became homogeneous at the first postoperative week. The HHK scaffold material was degraded into particles that was phagocytosed by macrophagocytes and multinucleate giant cells at the third week. U0|18|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the relation between Shp-2 （a cytoplasmic tyrosine phosphotase） and matrix metalloproteinase-1 （MMP-1） in the migration of mouse embryo fibroblast cell. Methods: Shp-2 -/-embryo fibroblast was separated from Shp-2 knockout mouse on E10. 5, and Shp-2 cDNA was transfected into Shp-2 -/- cell. The cell migration was observed with the wound healing, the MMP-1 expression and secretion was analyzed by immunoblotting and immunoprecipitation. The activation of MMP-1 in the supernatant was detected with type I collagenase activity assay after the stimulation of IL-1α compared with E10. 5 mouse embryo fibroblast cell（Shp-2 +/+）. Results: There was obvious migration in Shp-2 +/+ cells and Shp-2 -/-R cells, but not in Shp-2 -/- cells. The shape of Shp-2 -/- cell was epithelial-like. The expression and secretion was increased in Shp-2 +/+ cells and Shp-2 -/-R cells, and it had not changed in Shp-2 -/- cell. The activation of MMP-1 was lower in Shp-2 -/- cells compared to the other cells. C0|20|0Updated：2026-03-12
- Abstract：<正> Objective : To investigate the effects of the transfection of NHE-1 ribozyme gene on the apoptosis of pulmonary artery smooth muscle cells （PASMC） in vitro. Methods: After NHE-1 ribozyme gene was designed, synthesized and then cloned into plasmid pLXSN, the recombined plasmid was tansfected into cultured rat PASMC. Expression of NHE-1 mRNA was detected with semi-quantitative RT-PCR. Intracellular pH （pHi） was measured by using fluorescence dye BCECF-AM. Cell cycle was measured with aid of flow cy-tometric DNA analysis. Cell apoptosis was observed with electron microscopy and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNED respectively. Results: The NHE-1 mRNA expression level and pHi value were significantly lower in PASMCs transfected with NHE-1 ribozyme gene than those transfected with pLXSN or without transfection. Meanwhile, the apoptosis rate of cells transfected with NHE-1 ribozyme gene was increased significantly. Morphology of cell apoptosis was observed in the cell0|17|0Updated：2026-03-12
- Abstract：<正> Objective: To discuss the DNA-strand breaks at early stage of middle cerebral artery occlusion/ reperfusion （MCAO/R）. Methods: Neurons number and morphologic change were observed by Nissl stain method, and DNA strand breaks were in situ detected by using DNA polymerase- I Klenow fragment-mediated nick end-labelling method （Klenow method）. Results: Six hours after reperfusion, a few neurons in damaged regions appeared morphologic changes while a few Klenow-positive cells were detected （P<0. 01）. Twenty-four hours after reperfusion lots of neurons showed morphologic change while the number of Klenow-positive cells immediately and remarkably increased （P<0. 01）. Seventy-two hours after reperfusion the number of neurons decreased significantly and the number of Klenow-positive cells was also less than that in 24 h （P<0. 05）. Conclusion: ① 24 h after reperfusion when the number of Klenow-positive cells reached peak value, DNA single-strand breaks （SSBs） took place in many Klenow-positive cells, and presumed0|5|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the polyadenylation of mRNA in E. coli. Methods: The mRNA of E. coli was enriched from the total RNA with oligo（dT）-cellulose, prior to reverse transcription using oligo（dT）18 as the primer. Double-stranded cDNA was subsequently synthesized, which was subjected to digestion with Sau3A. I to produce multiple gene fragments for ligation with the adapters. PCR was carried out in 10 groups according to 10 different pairs of the selective primers, and the PCR products were then cloned into T-vectors. Results: More than 100 gene fragments had been cloned, 30 of which were sequenced. Conclusion: Polyadenylation of E. coli mRNA may not be a biochemical curiosity but a general attribute of bacterial mRNA.0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To explore the peculiarities of kidney transplantation in elderly patients and define the perioperative managements. Methods: The clinical data of kidney transplantation in 29 patients older than 65 years were reviewed, the eldest being 84 years old and the mean age 68.1 years. Results: Four episodes of a-cute rejection （13. 80%） were encountered. FK506 toxicity occurred in one case （3. 40%） and lung infection in another （3. 40%）, who （along with the former 4 patients） all were cured subsequently. In one case, the kidney graft was removed for thrombogenesis of the renal artery. The 1- and 3-year patients/grafts survival of 100% and 96. 5% respectively was achieved, with the longest survival exceeding 5 years. Conclusions; Old age was not the absolute contraindication for kidney transplantation. Strict observance of the indications of kidney transplantation and donor selection with well-matched tissue-typing are crucial in elderly patients. Adequate application of immunosuppressants and effect0|13|0Updated：2026-03-12
- Abstract：<正> Objective: To study the pathological basis of right atrial fibrillation in rheumatic heart disease （RHD） patients with atrial fibrillation （AF）. Methods: Twenty-nine patients with mitral valve replacement of RHD were divided into AF group （n = 13） and sinus rhythm group （SN group） （n = 16）. There was no significant statistical difference in clinical factors between the 2 groups. During the operation of valve replacement , the samples of right atrial appendages were taken and the qualitative and quantitative study were made by light microscopy and electron microscopy. Results: （1） Light microscope: The interstitial fibrosis and the arrangement of myocardium was more disordered in AF group than that in SN group. However, no statistic difference was found in interstitial fibrosis and cellar hypertrophy degree between the 2 groups. （2） Electron microscope: Mitochondrial crosta broke and dissolved obviously in AF group. The mitochondrial volume in AF group was smaller than that in SN group. Volume density, a0|11|0Updated：2026-03-12
- Abstract：<正> Objective: To provide the virtual model of the temporal bone for improving 3-dimension （3D） visualization of the inner ear. Methods: Plastination technique was used to make equidistant serial thin sections 1. 0 mm in thickness. On SGI workstation, a Contours + Marching Cubes algorithm was selected to reconstruct the temporal bone and intratemporal structures in 3D, then to view the middle ear, inner ear, and intratemporal structures which imitate the scenes observed by the traditional endoscopy. Results: The virtual model of the temporal bone was successfully constructed, with all reconstructed structures being represented individually or jointly and being rotated continuously in any plane. Virtual endoscopy improved 3D visualization of the middle ear, inner ear, and intratemporal structures. Conclusion: The reconstructed model can be used for the medical students to rehearse or review the surgeries on this part and for the surgeons to develop a new approach for operation. Virtual otoscopy stands as a p0|7|0Updated：2026-03-12
- Abstract：<正> Objective: To evaluate subtotal laryngectomy with preservation of monoarytenoid cartilage to reconstruct laryngeal function in the treatment of advanced laryngeal cancer. Methods: We retrospectively reviewed 48 patients with advanced laryngeal cancer （T3 or T4 on the primary site） treated by subtotal laryngectomy with preservation of monoarytenoid cartilage to reconstruct laryngeal function. Eighteen of them underwent neck dissection. Ipsilateral recurrent laryngeal nerve was preserved during this surgery. The cricoid cartilage was anastomosed to hypopharynx accordingly. Results: The overall 3- and 5-year survival rates were 81. 25% （ 39/45） and 66. 67 %（32/48 ）, respectively. All patients had good phonation and swallowing function after surgery. Decannulation rate was 93. 75%. Conclusion: Subtotal laryngectomy with preservation of monoarytenoid cartilage is satisfactory for treatment of elected advanced laryngeal cancers. The effect of this surgery is more satisfactory than that of the near-total laryn0|7|0Updated：2026-03-12
- Abstract：<正> Objective: To evaluate the role of colonic interposition for reconstruction of the esophagus. Methods: From April 1979 to October 1998, Thirty-one patients with benign esophageal stenosis and 15 patients with esophagus carcinoma received the colonic interposition for esophageal reconstruction. There were 5 patients with the esophagus carcinoma after subtotal gastrectomy. Four of 5 cases received colonic interposition graft from the esophageal bed after the esophagus was pulled out without thoracotomy. Results: There was no perioperative death. All 31 cases of the benign esophageal stenosis got long term survival and normal life. The 3- and 5-year survival rates of patients with the carcinoma of the esophagus were 63. 6% and 40. 6% respectively. Conclusion: The colonic interposition graft is a choice for the patients with the cervical stenosis of the esophagus, especially for those already had subtotal gastrectomy. Using the left colonic artery as the supporting vessel and using the colon segment in an i0|9|0Updated：2026-03-12
- Abstract：<正> Objective: To study the expressions and clinical significances of PCNA, EGFR, Bcl-2 and Bax in thymoma. Methods: The expressions of EGFR, PCNA, Bcl-2 and Bax in 46 cases of thymoma and 11 cases of normal thymus were detected with S-P immunohistochemistry. The results were analyzed with the pathologic indexes. Results: The positive rates of EGFR, Bcl-2 and Bax in normal thymus were 18. 2%, 9.1%, and 18. 2% respectively, while in thymoma were 71. 7% , 41. 3% , and 15. 2% separately. The expression of EGFR in thymoma was significantly correlated with Masaoka staging and Levine classification. The survival rate of EGFR negative patients was significantly higher than that of EGFR positive patients （P<0. 01）. PCNA labelling index was significantly higher in thymoma as （4. 00±1. 87）% than in normal thymus, （2. 68± 0. 62）% , which was significantly correlated with Levine classification. The expression of Bcl-2 in thymoma was also significantly correlated with Levine classification. The survival rate of Bcl-20|11|0Updated：2026-03-12
- Abstract：<正> Objective: To investigate the association of the transportation characteristics of nolatrexed in tumor cells with its drug sensitivity. Methods: The sensitivity of 3 tumor cell lines, C6, SRS82 and LoVo, to nolatrexed were determined by growth inhibition study. After exposure to 20 μmol/L nolatrexed at different time intervals ranging from 0 to 30 min, or to nolatrexed at different concentrations ranging from 0 to 40 μmol/L for 10 min, the intracellular drug concentration was measured using high-performance liquid chro-matography. Results: C6 was the most sensitive cell line among the three, with sensitivity 6. 8-fold and 13. 8-fold those of SRS-82 and LoVo cells respectively. Transportation of nolatrexed in the 3 cell lines were qualitatively similar, which rapidly achieved steady-state within 5 min, and linear relationship between the intracellular and extracellular drug concentration was observed. The intracellular steady-state level achieved in C6 was significantly higher than those in the other t0|8|0Updated：2026-03-12
- Abstract：<正> Objective: To assess MRI in the evaluation of enlarged superior ophthalmic vein （SOV）. Methods : MRI manifestations and etiology of forty-six patients with enlarged SOV were analyzed. Results: SOV enlargement was noted to occur in carotid-cavernous fistula, ophthalmic Graves’ disease, Tolosa-Hunt syndrome, inflammation at the apex of the orbit, orbital pseudotumor and thrombosis of cavernous sinus. The dilated vein appeared as signal void tubular shadows on both T1 and T2 weighted images. The diameter of the enlarged vein was 3. 5 - 6. 0 mm. Extraocular muscle enlargement, orbital pathologies, enlarged carotid cavernous sinus etc were also revealed by MRI. Conclusion: The dilated SOV may be well demonstrated by MRI. The etiological diagnosis of enlarged SOV can be made in combination with the associated findings.0|7|0Updated：2026-03-12
- Abstract：<正> Objective: To establish a systematic framework for selecting the best clustering algorithm and provide an evaluation method for clustering analyses of gene expression data. Methods: Based on data structure （internal information） and function classification （external information）, the evaluation of gene expression data analyses were carried out by using 2 approaches. Firstly, to assess the predictive power of clustering algorithms, Entropy was introduced to measure the consistency between the clustering results from different algorithms and the known and validated functional classifications. Secondly, a modified method of figure of merit （adjust-FOM） was used as internal assessment method. In this method, one clustering algorithm was used to analyze all data but one experimental condition, the remaining condition was used to assess the predictive power of the resulting clusters. This method was applied on 3 gene expression data sets (2 from the Lyer’s Serum Data Sets, and 1 from the Ferea’s Saccharomyces0|12|0Updated：2026-03-12
- Abstract：<正> Objective: To understand the present conditions of Children’s social adaptive capacity. Methods: Using the social viability measuring list on infants-junior middle school students revised by Zuo Qi-hua, the social viability and its influence factors were investigated on 628 Children in 7 kindergartens of 4 cities in China. Results: The general trend of development of Child’s social adaptive capacity was fairly good. The relevance ratio on the edge level was 0. 3%. Of them 16. 4% and 7% were excellent and extraordinary intelligence respectively. The family environment had played a very important role in child’s social adaptive capacity. Conclusion: The research revealed that in the respect of training a child’s social adaptive ability, the initiative of each family should be brought into full play and we should surmount the negative influence, and solve the contradiction between releasing one’s control and taking care of everything, and arouse the conscious activity of the child, and ensure the unity and0|24|0Updated：2026-03-12
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