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Volume ， Issue 3 ， 1989
- Abstract：<正> Accurate typing of the different human papillomavirus types is csscntial in view of the differ-ent pathological potential of the common virus types of human papillomavirus （HPV） present in thecervix. We have developed hybridization, washing and autoradiography conditions that minimize thecross-hybridization among different specific types of HPV so as to allow clear - cut type assignmentthrough practical dot blot hybridization technique using nylon membrane and 35S - labeled HPV - 16DNA probe. Under these conditions seventeen of thirty （56.7%） of squamous cell carcinomas of thecervix uteri obtained from Tianjin women were detected in the presence of HPV - 16 DNA.0|8|1Updated：2026-03-12
- Abstract：<正> Extract of the eschar tissue taken from the rat which had been given full-thickness burn of20% TBSA was found to be inhibitory to the respiratory function of the mitochondria and theenergization of the inner membrane. The inhibition was also demonstrated by the 1: 2 and 1: 4and 1: 8 dilutions of the eschar extract. The extract of normal skin only showed weak inhibitory cf-fect. The 1: 4 dilution of normal skin had no （?） on mitochondrial functions. There is a widegap between the malonaldehyde （MDA） contents of these two kinds of extracts. An apparent posi-tive correlation was found between MDA content and degree of inhibition. However, on close ex-amination, it was found that the MDA content is not the direct cause of the decrease of therespiratory control ratio （RCR）. The increase of MDA content is probably related to the destruc-tion of the lipid bilayer of the mitochondria. Data from the energization experiment show that theenergization process is compromised when the destruction of the membrane has proceeded to a cer-tain degrce. The inhibition of mitochondrial respiratory function becomes evident when encrgizationfunction is still normal. Therefore at least two mechanisms are operating in the inhibition process.Further work is needed to elucidate the problem.0|1|0Updated：2026-03-12
- Abstract：<正> In order to find atriopeptin （AP） analogues with more potent and longer-lasting effects,we have synthesized rat APⅢ and its three analogues A1, A2, A3 , and assayed their bioactivitiesin anesthetized rats. The results demonstrate that the synthesized APⅢ has powerful diuretic,natriuretic effects, and can remarkably lower arterial blood pressure, while the heart rote remainsunchanged in the process. The aforementioned effects of APⅢ are dose-dependent. The hypotensionof APⅢ is independent of the diuresis caused by APⅢ. The three analogues’ of APⅢ are all 24peptides. A1 and A3 had no bioactivities, while A2 possessed atriopeptin-like, but weaker activities.The results suggest that the 5th, 6th and 7th amino add residues and their configurations in thepeptide chain of AP Ⅲ are crucial for eliciting bioactivities.0|2|0Updated：2026-03-12
- Abstract：<正> Seven monoclonal antibodies （McAbs） specific for Plasmodium falciparum （Pf）, humanimmune sera of three individuals, two adults and one child, living in a malaria endemic area inHainan Island of China and immune sera of BALB/c mice immunized with the erythrocytic stages ofPF were used to identify the target antigens of asexual blood stages of Pf. The 125I-labeled surfaceantigens with apparent molecular weights of 125, 115, 83, 74 and 67 KDa were mainly recognizedby McAbs 93A3, 94B5, 92D4 and 93D4, but the 125I-labeled surface antigens of 200 and 19 KDawere recognized by McAb 94Dl alone. In addition, the 35S-methionine- labeled target antigens of183, 125, 83 and 55 KDa were also recognized by McAbs 93A3, 94B5 and 94C3. The McAb21E3 against the culture supernatant antigen of Pf only recognized the antigen of 125 KDa labeledwith 15S-methionine. The human immune sera from the adulst living in a malaria endemic area andmouse immune sera not only immunoprecipitated the target antigens which were recognized by theMcAbs but also immunoprecipitated the polypeptides of 140, 100 and some lower - molecularweight. But these target antigens recognized by McAbs, adult immune sera of and mouse immunesera could not be immunoprecipitated by human immune serum of the child with a primary infec-tion. With the antigen competition assay, the unlabeled antigens of Pf could strongly inhibit the im-mune reaction between the McAbs and the 125I-labeled antigens of Pf. It suggested that the antigensrecognized by McAbs and polyvalent immune sera were specific target antigens.0|3|0Updated：2026-03-12
- Abstract：<正> The changes of sclenium metabolism, glutathione peroxidase activity and lipid peroxidescontent in the tissues of rats suffering from 30% TBSA full thickness scalding were observed in thefirst 7 days after injury. It was found that selenium content in the rat tissues decreased remarkably af-ter injury, which in turn resulted in serious reduction of glutathione peroxidasc activity and significantincrease of lipid peroxides in the scrum, crythrocytcs and liver. However the muscular tissue showedno significant changes. These facts imply that after burn injury, the body is in a state of selenium deficiency, the lossof selenium might be responsible for the reduction of anti - peroxidation ability of glutathioneperoxidase, and conscqucntly there is an increase of lipid peroxides in the tissues. Only the musculartissue is insensitive to lipid peroxidation. It is believed that the reduction of anti-peroxidation abilityof glutathione peroxidasc after bum injury might be one of the main causes to intensify, the injury re-suiting from free radicals.0|12|1Updated：2026-03-12
- Abstract：<正> In the present study, the conjugate of mitomycin C （MMC） with a murine monoclonalantibody against gastric cancer, MG11, was prepared and its cytotoxicity on gastric cancer cell lineKATOⅢ was determined. MMC was modified with glutaric anhydride to introduce a carboxylicgroup at N-lα position. The MMC derivative was then treated with N-hydroxysuccinimide anddicyclohexylcarbodimide to from MMC active ester, which was combined with monoclonal antibodyMG11 for preparation of MG11-MMC conjugate, which showed highly selective cytotoxicity uponhuman gastric cancer cell line KATOⅢ in culture. In the 48h exposure test, the cytotoxicity ofMG11-MMC conjugate on target cells was found to be similar to that of free MMC. The conjugatehad only a little effect upon nontarget cells （human normal embryonic lung cell line SL7）. The re-sults suggest that the selective cytotoxicity on target cells of MG11-MMC is mediated by theantibody.0|5|0Updated：2026-03-12
- Abstract：<正> The experimental models of craniocercbral wounds caused by 7. 62 mm bullets, i. e. thepenetrating craniocerebral injury, the tangential brain injury and the tangential skull injury, were es-tablished in dogs. The craniocerebral ballistics, craniocerebral pathology, serum and cerebrospinal flu-id total lactate dehydrogenase, blood-brain barrier permcabalities, and the pathophysiology ofcardiovascular and respiratory systems were studied. These results suggest that: 1. These injuries ofhigh-velocity missile can all cause general brain damage and intracranio-hematomas ; 2. The severityof the wound depends on the site of the injury, the kinetic energy of the missile force and the effectof the temporary cavity ; 3. The brain injury can seriously damage the blood brain barrier, leadingto brain edema ; 4. The dysfunction of respiratory and cardiovascular system is the fatal complicationendangering the life of the subjects ; 5. Estimating serum and cerebrospinal fluid total lactatedehydrogenase is a simple and valuable way to judge the severity and prognosis of this injury.0|6|1Updated：2026-03-12
- Abstract：<正> Experiments were performed on 25 rats. Putative serotonergic antagonist, Cyproheptadine（Cyp）, was applied microiontophoretically on the nucleus parafascicularis of the thalamus （Pf） andthe effect of Cyp on Pf nociceptive neuronal discharge was observed. The result suggested that Cypcan increase the spontaneous and evoked discharge rates of Pf nociceptive neuron, and that Cypmay antagonize the inhibitory effect of endogenous serotonin on the spontaneous and evokeddischarges of Pf nociceptive neurons.0|3|0Updated：2026-03-12
- Abstract：<正> It was first found that nickel foam wasused as a protective material against blast in-juries. Nickel foam was made of plastic in1cm thickness forged and nickeled, withmeshes of 26-35/in, and the diameter ofwhich is more than 0.1mm or more. Sixty-one animals, including twenty-nine mongreldogs, twelve guinea pigs and twenty rats,were used in this experiment which were di-vided into blast injury group （control guoup）and nickel foam group （protective group）. The result showed that the nickel foamwas crashed due to the impact of the steel0|2|0Updated：2026-03-12
- Abstract：<正> Immunocytochemical localization of 5-hydroxytryptamine （5-HT） containing cells of the guinea-pig pancreas was studied by means of PAP-glucose oxidase-DAB-nickel （PAP-GDN） and PAPstaining methods on paraffin sections. In this study we demonstrated the presence of 5-HTimmunoreactive cells in the endocrine （islets） and exocrine pancreas of the guinea-ping. Cells exhib-iting 54-HT immunoreactivity were found in the center and the periphery of the pancreatic islets. The5-HT immunoreactive granules were evenly distributed throughout the cytoplasm, but was faint inthe nuclei. Cells of this type were also distributed in the exocrine pancreas. Grouped or single or single cellsoccurred intercalatedly between acinar cells, as well as in the epithlium of ducts and in the connectivetissues near the ducts or acini.0|10|0Updated：2026-03-12
- Abstract：<正> The dynamic changes of liver microsomal drug-metabolizing system （MDMS） andlipoperoxidation were studied in scalded rats. The effects of treatment with vitamin E and silybinwere also evaluated. The results showeed that liver microsomal cytochrome P-450 content, and p-nitroanisole demethylase （P-NOD） and aniline hydroxylase （AH） activity decreased markedlypostburn. On the contrary, liver lipoperoxide and mierosomal lipoperoxidation increased significantlyafter scalding. Both the increase of liver lipoperoxide and mierosomal lipoperoxidation and the de-crease of MDMS activity were prevented by vitamin E and silybin treatments.0|7|0Updated：2026-03-12
- Abstract：<正> Antiserum against PreS2 peptide was raised with a synthetic polypeptide from the rabbits.The anti-preS2 antibody and polymerized human serum albumin were used as reagents in aradioimmunoassay to detect preS2 and polymerized human serum albumin bindingactivity respectively. Both were absent in patients with hepatitis A or HBsAg negative chronic liver di-seases. In biopsy - proven patients with chronic active hepatitis （CAH）B, prevalences of bothmarkers were significantly higher at exacerbation that at remission stage of the disease, and so werein CAH than in chronic asymptomatic HBV carrier （AsC） with normal histology. Besides, the pre-valences were significantly higher in HBeAg positive group than in anti-HBe positive group.However, the polymerized human serum albumin binding activity and the preS2 were undoubtedlynot the same, as the prevalence of the latter was only 56.7% of the former.0|6|0Updated：2026-03-12
- Abstract：<正> The effects of chronic hypoxia on calmodulin levels of lung tissues and small pulmonary ar-terial walls were studied in young pigs, The tissue specimens of hypoxic animals were obtained underhypoxic conditions. The following results were collected:（1） The swine exposed to chronic intermittent hypoxia showed a significant pulmonary pressor re-sponse at a simulated high altitude of 4000 m.（2） A higher level of calmodulin was found in the lung tissues of chronic hypoxic animals. It maybe related to the increased release of some vaosactive substances from pulmonary non-muscularcalls.（3） No significant difference of calmodulin level of small arterial walls was demonstrated between theexperimental animals and the control.The findings suggest that pulmonary vasoconstriction due to hypoxia is not likely to be associatedwith obvious change in calmodulin level in the smooth muscle of blood vessels.0|2|0Updated：2026-03-12
- Abstract：<正> Romberg’s disease is the progressive atro-phy of a half of the face. A "dermis-fat-leftskin flap （DFLSF）" from lower abdominalwall was designed and grafted to the atrophichemiface associated with vascularanastomosis. The result is satisfactory. The DFLSF consists of 2 parts; one partof the flap consists of the dermis and thesubcutaneous fat and is used to fill up the de-pressed region of the atrophic hemiface, andthe other is a regular skin flap with a gappingin the center to cover the naked portion of the0|5|0Updated：2026-03-12
- Abstract：<正> The relationship between gastric mucosal blood flow （GMBF） and gastric acid secretionwere studied in rats by using secretory stimulant （pentagastrin）and inhibitor（cimetidine）. GMBFwas measured by Laser Doppler flowmetry （LDF） and gastric mucosal pH determined by microglasspH electrode. GMBF increased and gastric mucosal pH decreased significantly after intravenous injec-tion of 6μg/kg of pentagastrin; nevertheless GMBF decreased and gastric mucosal pH increasedmarkedly after intravenous administration of 100mg/kg of cimetidine. This indicates that pentagastrincan increase GMBF and gastric acid secretion, and cimetidine can decrease GMBF and gastric acidsecretion in rats, proving the close relationship between GMBF and gastric acid secretion in rats.0|4|0Updated：2026-03-12
- Abstract：<正> When the troops are attacked by nuclear weapons, the number of the wounded and thetype and the condition of the wounds will change with the equivalence and the way of explosion, thenumber of soldiers taking part, the area of the disposition of the troops, and the conditions of protectionof the personnel. Whether the wounded by nuclear weapons who is pouring in enormous amountcan be correctly classified in time has a very important relation in enhancing the effect of the first-aidand the treatment later on. We worked out a programme about the defined types and criteria of thewounded by nuclear weapons beforehand to be stored into the microcomputer. After nuclear cxplo-sion, it is necessary only to input the known data into the microcomputer from the key-board, thecomputer will immediately tell the number of the wounded of various types, the number of peopleand the time needed to perform the triage task and the surgical personnel needed to performthe operations, so that medical supporting programme can be selected or adjusted on time and the ef-ficiency and quality of the triage and first-aid work can be improved.0|6|0Updated：2026-03-12
- Abstract：<正> Sister chromatid exchanged （SCE） and chromosome aberrations in peripheral lymphocytesof 30 patients with cervical cancer and relative lengths of C-band in 20 patients were studied. Verysignificant increase in SCE, hypodiploidies and relative lengths of C-band of chromosome 9 were ob-served, and significant increases in polyploidies, structural aberrations and relative lengths of C-bandof chromosome 1 were also found in cancer patients as compared with the controls, with all in non-randomized distribution. No differende was noted in alterations between stage Ⅱ and Ⅲ cancers.These findings suggest that chromosomal instability in the cervical cancer patients may represent aningerent trait in the patient and be related to the increased susceptibility of the individual to malignan-cy.0|2|0Updated：2026-03-12
- Abstract：<正> Six hours after smoke inhalation injury in rabbits, the permeability of pulmonary vesselsand the aggregation of circulating platelets increased markedly accompanied with apparent patholog-ical changes in the trachea and lungs. Fifteen minutes after smoke inhalation injury in rabbits, an intravenous dose of ginsenosides or ketoprofenwas given to the animals respectively. 6 hours after medication, it was found that both the drugscould significantly alleviate the platelet aggregation, but only ginsenosides could alleviate theaugmentation of pulmonary vascular permeability and the pathological lesions in the trachea andlungs. In those rats injured by smoke inhalation, l hour after an intravenous dose of ginsenosides, theplasma PGI2 level was elevated and TXA2/PGI2 ratio decreased significantly.0|5|0Updated：2026-03-12
- Abstract：<正> The transport of sodium ions by erythrocytes and the plasma level of endogenous digitalis-like compound （EDLC） were assessed in 59 patients with essential hypertension before and after theadminstration of nifedipine and prazosin. 20 normal subjects were studied similarly and served as con-trol. It was found that （1） EH patients had a pronounced defect of both the active and passive trans-port of sodium ions by the erythrocytes; （2） a higher plasma level of EDLC was detected in EH pa-tients as compared with that of the control, but the changes of EDLC and soudium pump were notparallel; （3） after the administration of nifedipine and prazosin, the function of sodium pump wasmarkedly improved and the plasma level of EDLC decreased. In addition, the relationship betweenthe transport of sodium ions by erythrocytes and the pathogenesis of EH, and the effects of anti-hypertensive agents were discussed.0|11|0Updated：2026-03-12
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