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Volume ， Issue 2 ， 1988
- Abstract：<正> The in situ hybridization of HBV DNA with biotinylated probe informalin-fixed and paraffin-embedded liver tissue sections was performed and thelocalization and morphology of HBV DNA in various types of chronic liver diseases wereobserved.25 out of the 75 cases studied were HBV DNA positive （33.3%）.HBV DNA inhepatocytes was demonstrated as numerous coarse grains in cytoplasm and occasionally afew grains in individual nuclei.The distribution of the HBV DNA positive hepatocytes inthe sections was in 3 types,i.e.,the diffuse type （Type I,4/25,16%）,the focal type （Type Ⅱ,20/25,80%）,and the scattered type （Type Ⅲ,1/25,4%）.The HBV DNA grains incytoplasm might be condensed （Type a） or sparsed （Type b） depending on the number of theHBV DNA grains.The different distribution patterns and the amount of HBV DNAgrains might be a reflection of the replication activity and the state of infectivity of HBV.HBV DNA positive hepatocytes were found only in the tissues contiguous to the cancertissue in cases with hepatocellular carcinoma with cirrhosis.0|8|0Updated：2026-03-12
- Abstract：<正> Supernatants collected from the short term in vitro serum-free cultures ofPlasmodium falciparum （Pf） schizont-infected erythrocytes were detected and analysed bycounterimmuno-electrophoresis （CIE） and enzyme-linked immunoabsorbent assay （ELISA）.Soluble antigens were purified from the supernatants by immunoabsorbent chroma-tography. It was found that soluble antigens similar to the sonicated Pf antigens inimmunospecificity were present in the supernatants and no erythrocyte membrane antigenswere detectable in the affinity-purified supernatant antigens （Ps-Ag）.0|0|0Updated：2026-03-12
- Abstract：<正> Intracellular recording of the rat hippocampal slices was established and usedto analyse the effects of glucocorticoid on resting membrane potential as well as inputresistance of the cells in CA1 and CA2 of hippocampal slices.The results indicate thatwithin 1-2 min the steroid hyperpolarized some of the hippocampal neurons with atendency of increasing the cells’ input resistance,suggesting that glucocorticoid caninfluence the function of central neurons in a non-genomic pattern,and the underlyingmechanism involves the change of resting membrane potential.0|1|2Updated：2026-03-12
- Abstract：<正> An experimental study on models of traumatic wounds and scars made oncorneas of 116 rabbits indicates that“Hua yunning”has no adverse effect on healing ofcorneal traumatic wound.Its therapeutic effect on corneal nebula was singificant and farbetter than that of dionine and sodium iodide.On pathological,histochemical and electronmicroscopic examinations it is found that the action of Huayunning on corneal scar seemsto be inhibition of the abnormal proliferation of fibroblasts in the stroma and synthesis ofcollagens,enhancing maturation of fibroblasts and causing the cells,fibers and groundsubstance in the corneal stroma to become normal or nearly normal.0|1|0Updated：2026-03-12
- Abstract：<正> HBV antigens were immunochistochemically demonstrated with colloidal goldprobes in the liver from 204 individuals with chronic asymptomatic HBV infections.Theresults were comparaed with those from 46 patients with chronic hepatitis type B （CHB）.Itwas found that the prevalence of the intrahepatic antigens had no sifnificant differencebetween chronic asymptomatic hepatitis （AsH） and chronic asymptomatic carriers （AsC）.However,in the prevalence of the intrahepatic HBV antigens,a significant difference didexist between AsH and CHB which had similar liver pathology.HBsAg and HBcAg were94.2% and 46.2% in AsH,and 32.6% and 17.4% in CHB,respectively.These resultssuggest that the high prevalence of viral antigens in the liver from individuals with chronicasymptomatic HBV infection might be resulted from accumulative changes during along-term infection.0|1|0Updated：2026-03-12
- Abstract：<正> Changes of spinal evoked potentials （SEP） were observed in rats by stimulatingthe sciatic nerves on both sides under various pressure conditions （normal,hyperbaric,rapid decompression,safe decompression,and recompression treatment） and in acuteanoxia.It was observed that occurence of acute decompression sickhess （DCS） was relatedwith changes of SEP.The changes of SEP late components （N3,P3,N2） occurred earlierthan SEP early components （P2,N1,P4）.It was shown that in using SEP method tomonitor DCS,late components were more sensitive as compared with early components andspecial attention should be given to the amplitudes of SEP.As soon as changes of latecomponents appeared,acute DCS would follow.If recompression would be given at thispoint,DCS could be reduced or eliminated.When early components showed changes,DCSwas in such a stage as to cause immediate death of the animals.Recompression had noeffects at this point.The late components and early components,therefore,were ofsignificantly different value in reflecting the level of DCS.N2 wave was an important key.The results suggest that using the SEP method to monitor acute DCS is a sensitive andaccurate way.0|1|0Updated：2026-03-12
- Abstract：<正> Using turbidimetry and electronmicroscopy,we examined the effects oftetrandrine （Tet） on the aggregation and morphological changes of rabbit platelets.It wasfound that Tet could markedly inhibit the platelet aggregation induced by ADP,collagenand arachidonic acid （AA） in a dose-dependent manner.The IC50 （50% inhibitoryconcentration） was 38.9,30.3 and 44.3μg/ml respectively.The platelets pretreated with Tetcould not be activated by collagen,their morphological changes and granule release didn’toccur and their normal structure was well preserved.In addition,dipyridamole couldenhance the inhibition of Tet on platelet aggregation,while increased extracellular calciumcould weaken this inhibition.0|2|0Updated：2026-03-12
- Abstract：<正> Medulloblastoma is a primitive embryonaltumor.It accounted for about 4% of interacran-ical tumors,but the mortality is very high.Theultrastructure and tissue origin of this tumorare still not quite clear.Recently we inves-tigated 8 medulloblastoma specimens confirmedby pathology.The results were as follows:The tumor tissue obtained from the operat-ing room was immediately cut into small pieces0|2|0Updated：2026-03-12
- Abstract：<正> Hemagglutination inhibition test （HIT） was performed with Chen viurs strain ofhemorrhagic fever with renal syndrome （HFRS） to detect antibodies in the sera of patientsand animals from various endemic regions in China,and compared with immunofluoresc.ent assay （IFA）.The results demonstrate that HIT is sensitive,specific,reproducible andsimple.It is applicable to epidemiological seroinvestigation as well as clinicalsero-diagnosis,especially to the grass-roots medical units.0|6|0Updated：2026-03-12
- Abstract：<正> In order to establish a simple and accurate method for the determination ofangiocardiographic ejection fraction （EFa）,the end-diastole,end-systole,the first third andthe first half of the ejection phase on cineventriculograms of the right anterior oblique 30°（RAO 30°） in 42 subjects were analyzed.We found that the percentage of total axisshortening （T%Δa） and EFa were in positive correlation.Their linear regression equationis EFa=0.1712+1.3303T%Δa （r=0.880） and the quadratic equation is EFa=0.0396+2.4874T%Δa 1.9787 （T%Δa）2 （r2=0.981）.The EF obtained in another 10 subjects by the linearand quadratic regression equations and area-length method are 0.469±0.176,0.464±0.208and 0.482±0.208,respectively.（P>0.05）.It is our opinion that the regression method issimple and the results are positively correlated with the area-length method.It can be usedas a new method for calculating EFa.0|2|0Updated：2026-03-12
- Abstract：<正>In reeent years banding analyses on ehroos;omes of human beings,miee and othermammalswith restrietion enzymes have beenrePorted abroad:however,there was no rePortabout ehromosomes of Parasites with the sameteehnique.The authors try to study the ehromo-someG一banding of SPirometra mansoni byusing restrietion endonuelease（Haelll）andanalyse the bands with mierosPee-troPhotometer.0|5|0Updated：2026-03-12
- Abstract：<正> DNA content of 38 hepatocellular carcinomas （HCC） was quantitativelydetermined by means of image analysis technology.The results showed that DNA contentin HCC was correlated well with the tumor size,capsule breaking,cancerous thrombusforming and clinical prognosis,that is,the higher DNA content was,the more malignanceappeared,but not with the clinical staging and pathological grading of HCC.66.7% ofDNA diploid/near diploid HCC were less than or equal to 3 cm in diameter,whereas 75%of DNA aneuploid HCC were more than 3cm in diameter （P<0.05）,suggesting that HCC at3cm in size may be an important period when the change of biological characteristics wouldoccur.This study indicates that the quantitative analysis of DNA content can provide avaluable supplement to morphology in predicting the biological features and objective basesfor assessing clinical prognosis of HCC.0|15|1Updated：2026-03-12
- Abstract：<正> The affinity between 2-iminobiotin and avidin decreases with the decreasing ofpH.According to this property,avidin was purified in its natural state without alterationof its properties with affinity chromatography on 2-iminobiotin-AH-sepharose 4B column.The purified avidin had high activity to bind with biotin,showed one band onpolyacrylamide electrophoresis,and was much purer than the products commerciallyavailable.0|20|0Updated：2026-03-12
- Abstract：<正> Platelet aggregation ratio （PAR） was assessed in 45 patients with severe viralhepatitis （SVH） with Born’s method.The PAR of normal individuals,patients with acuteicteric hepatitis,and those with chronic active hepatitis were also studied to serve ascontrols.It was found that PAR decreased markedly in SVH patients,which was marked in theearly stage of SVH,more marked in the intermediate stage,and most marked in the latestage.The changes of PAR were in negative correlation with those of serum total bilirubin,and in positive correlation with those of prothrombin activity.The reduction of PAR inSVH patients might be ascribed to the defect of platelets themselves as revealed bycross-over experiments.The results of this study indicate that PAR may be useful in judging the severity ofSVH patients since the degree of PAR reduction parallels closely with the severity of liverdamage.Finally,the condition of platelets in vivo and in vitro in SVH patients werediscussed.0|7|1Updated：2026-03-12
- Abstract：<正> The influence of actinomycin D and puromycin on the mediation of cellularimmunity by mouse S180 ascitic tumor cell-specific iRNA was investigated with 3H-Leu-LAItest as an index.The results showed that mouse splenic lymphocytes sensitized with iRNAmaintained their LAI activity after the translation or transcription was blocked bytreatment with puromycin or actinomycin D respectively;and that when first treated withactinomycin D or puromycin,then incubated with iRNA and relevant antigen,thelymphocytes did not show any LAI activity.These results suggest that transcription andtranslation processes in lymphocytes are involved in the mediation of LAI reaction byiRNA,and during the treatment of cancer,the effectivity of iRNA would be reduced by theprevious administration of tumoricidal agents which are suppressive to the RNA and/orprotein synthesis in patient’s lymphocytes.0|8|0Updated：2026-03-12
- Abstract：<正> We report herein effects of varying dietary zinc contents on burns healing andcorrelation between serum zinc and serum alkaline phosphatase （AKP） activity and serumprotein in 15% total body surface area （TBSA） of Ⅲ°burned Lop rabbits.Serum zincconcentration in the rabbits given a low zinc diet for 2 weeks and those with burnsdecreased significantly,and further decreased and failed to return to normal in the rabbitwith a low zinc diet and burns.On the contrary,serum zinc was recovered rapidly tonormal in the groups fed an adequate zinc diet.Similar changes were found in serum AKPactivity.Postburn total serum protein and albumin contents were markedly decreased anddid not return to normal on d 15.Burns healing in the high zinc group was over 10 dfaster than that in the low zinc group.Serum zinc was found to be well correlated withserum AKP activity and serum protein.These results strongly suggest that zinc might be afactor benefiting burns healing.We conclude that serum AKP activity could be used as adiagnostic criterion of postburn hypozincemia as well as a valid index in the assessment ofthe effectiveness of supplemental zinc.0|6|0Updated：2026-03-12
- Abstract：<正> The clinical and laboratory findings of 2197 cases of systemic lupuserythematosus（SLE）were reviewed and analyzed to reveal the incidence of each of theabnormal changes and evaluate its value and specificity in the diagnosis of the disease.According to the conditions of our country and the experience of many authors,a new set ofdiagnostic criteria for SLE was established and systematized into a computer program.Tested on 223 non-SLE cases and 92 cases of confirmed SLE,this new set of diagnosticcriteria had the rates of specificity and sensitivity of 91.9% and 95.7% respectively.It is considered that the new set of diagnostic criteria of SLE is characterized by itscomparatively comprehensive contents and its conformity to the conditions at home.It isespecially helpful for the early diagnosis of SLE.0|3|0Updated：2026-03-12
- Abstract：<正> By using immunofluorescent cytochemical method,EB-VCA-IgA antibody levelswere studied in 35 cases of nasopharyngeal carcinoma.It was found that VCA-IgAantibody level declined with the improvement of the patient’s condition and rose up withthe worsening of the disease in most cases.It is indicated that the change of EB-VCA-IgAantibody level might be used as an objective indication for the determination of 60Coradiotherapy effect.0|1|0Updated：2026-03-12
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