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      Volume 26 ， Issue 1 ， 2011

      • Differentiation of mouse embryonic stem cells into insulin-secreting cells in vitro

        Sui Jing~(1, 2), Jiang Fangxu~2, Shi Bingyin~(1) 1 Department of Endocrinology, First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, China 2 Center for Diabetes Research, Western Australian Institute for Medical Research, Centre for Medical Research, University of Western Australia, Perth 6000, Australia
        Vol. 26, Issue 1, Pages: 1-12(2011)
        Abstract：<正>Regenerative medicine,including cell-replacement strategies,may have an important role in the treatment of type 1 diabetes which is associated with decreased islet cell mass.To date,significant progress has been made in generating insulin-secretingβcells from pluripotent mouse embryonic stem cells（ESCs）.The aim of this study is to explore the potential of regulating the differentiation of ESCs into pancreatic endocrine cells capable of synthesizing the pancreatic hormones including insulin,glucagon,somatostatin and pancreatic polypeptide under proper conditions. Undifferentiated ES cell line was stably transfected with mouse RIP-YFP plasmid construction in serum-free medium using Lipofectamine? 2000 Reagents.We tested pancreatic specific gene expression and characterized these ESC-derived pancreatic endocrine cells.Most of these insulin-secreting cells co-expressed many of the phenotypic markers characteristic ofβcells such as insulinl,insulin2,Isletl,MafA,insulinoma-associated antigen 1（IA1） and so on,indicating a similar gene expression pattern to adult islet p cells in vivo.Characterization of this population revealed that it consisted predominantly of pancreatic endocrine cells that were able to undergo pancreatic specification under the appropriate conditions.We also demonstrated that zinc supplementation mediated up-regulation of insulin-secreting cells as an effective inducer promoted the development of ESC-derived diabetes therapy.In conclusion,this work not only established an efficient pancreatic differentiation strategy from ESCs to pancreatic endocrine lineage in vitro,but also leaded to the development of new strategies to derive transplantable islet-replacementβcells from embryonic stem cells for the future applications of a stem cell based therapy of diabetes.  
          
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      • Gao Yuling, Liu Yuqi, Lan Yunfeng, Wen Yi, Fang Zhou, Gao Jinliao, Wang Xueping, Wang Hongjuan, Li Yang Institute of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China
        Vol. 26, Issue 1, Pages: 13-20(2011)
        Abstract：<正>The noninfarcted myocardium underwent significant electrophysiological remodelling as part of the healed myocardial infarction remodelling.This study aimed at investigating the effects of nervous growth factor（NGF） on delayed afterdepolarizations（DADs） and triggered activity（TA） of the noninfarcted myocardium in the myocardial infarcted rabbit model.Rabbits with the left anterior descending coronary artery occlusion were prepared and recovered for 8 weeks（HMI group,n=9）.Other rabbits with myocardial infarction were infused NGF to the left stellate ganglion （HMI+NGF group,400 U/day for 8 weeks,n=8）.Myocytes were isolated from regions of the noninfarcted left ventricular free wall.Action potentials and ion currents were recorded with whole-cell patch clamp.The results showed that more DADs and TA events of HMI+NGF myocytes than that of HMI and Ctrl group.Iti and 7Ca-L of NGF+HMI myocytes were increased significantly compared with HMI and Ctrl cells,which contributed to DADs-related triggered arrhythmia.Comparing with HMI and Ctrl myocytes,significant prolongations of APD50 and APD90 in HMI+NGF myocytes were found.The results indicated the electrophysiological change of HMI myocytes with NGF infusion.It suggested that more events of DADs and TA in HMI myocytes with NGF treatment.The underlying mechanism may be involved in the increase of Iti and ICa-L.  
          
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      • Liu Jia~1, Han Junfeng~2, Li Dongying~3, Jian Rui~4, Rao XianCai~1, Chen Wei~1, Wang Jiali~1, Xu Xiaofeng~5, Hu Zhen~1, Zhang Junlei~(1) 1 Department of Microbiology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China 2 Institute of Immunology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China 3 Weifang Center for Disease Control and Prevention, Weifang 261041, China 4 Department of Pathogenic Biology, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China Department of Medical Genetics, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China
        Vol. 26, Issue 1, Pages: 21-28(2011)
        Abstract：<正>The ribosomal protein S29 also known as RPS29,is not only a component of the 40S subunit of ribosome,but also involved in embryonic development,oncogenesis and other pathologic conditions.However,rare commercial antibody against RPS29 restricts the discovery of precise physiological and pathological function of this protein.In this study, the whole RPS29 gene was inserted into plasmid pGEX-6p-l to express glutathione’s transferase（GST） fusion proteins in Escherichia coli（E.coli） strain BL21.High yields of soluble recombinant proteins were obtained.Mice were immunized with the recombinant RPS29 protein.The serum from the immunized mice could specially react with purified recombinant RPS29 proteins and native RPS29 proteins in CCE cells by western blotting,immunofluorescence staining and flow cytometric analysis.Further more the polyclonal antibodies also reacted specifically with human cell strain ECV304,which showed typical cytoplasmatic fluorescence.The polyclonal antibodies we prepared would be an available tool for studying the roles of RPS29 in embryonic development and human diseases.  
          
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      • Dexamethasone plus lenalidomide in treatment of multiple myeloma

        Deng Wei, Ya Mingxi~, Xu Jianwang, ZhangHao, Li Ming, Li Pei Institute of Hematology and Department of Hematology, First Clinical Hospital, Lanzhou University, Lanzhou 730000, China
        Vol. 26, Issue 1, Pages: 29-33(2011)
        Abstract：<正>Combination of lenalidomide with dexamethasone（LD） is of high effectiveness for treatment of multiple myeloma（MM）,resulting in an improved overall survival,response rate,and time to progression in relapsed or refractory MM.Adverse events,especially venous thromboembolism,were generally well tolerated.In addition, lenalidomide plus dexamethasone is a potential therapy in maintenance of myeloma.  
          
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      • Xu Gaofeng, Xie Wanfu~, Li Ruichun, Wang Maode Department of Neurosurgery, First Affiliated Hospital, Medical School, Xi ’an Jiaotong University, Xi’an 710061, China
        Vol. 26, Issue 1, Pages: 34-39(2011)
        Abstract：<正>Traumatic intracranial pseudoaneurysms（TIPA） are rare and pseudoaneurysm of P3 segment of the posterior cerebral artery（PCA） is extremely rarely reported.A 27-year-old man suffered from blunt head injury and underwent diagnostic procedure with head CT scans,cerebral CT angiograms（CTA） and digital subtraction angiograms（DSA）.An abnormal high-density lesion was detected at the left side of quadrigeminal cistern and a pseudoaneurysm showed at the P3 segment of PCA.The pseudoaneurysm was successfully treated with parent artery occlusion（PAO）.Making an exact preoperative diagnosis of traumatic intracranial pseudoaneurysms is of great importance.Endovascular techniques allow a safe approach to complex intracranial lesion,so endovascular management of PCA pseudoaneurysms becomes safe and effective.However,the pseudoaneurysm may not be amenable to coil placement under some conditions,thus,occlusion of the parent vessel may be a preferable treatment.  
          
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        Updated：2026-03-12

      • Submental tracheal intubation for resection of recurrent giant pituitary tumor:a case report

        Zhong Hejiang, Wang Yunling, Yang Tiande~ Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
        Vol. 26, Issue 1, Pages: 40-44(2011)
        Abstract：<正>Airway management in the patients who receive transmaxillary approach for resection of giant pituitary tumor presents a clinical challenge to the anesthesiologists.Oral or nasal route for tracheal intubation can interfere with surgical procedures.This report describes submental tracheal intubation for airway management in a patient who underwent resection of recurrent giant pituitary tumor via transmaxillary approach.Submental tracheal intubation is an adaptable and safe alternative technology for airway management during operation.  
          
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      • Significance of humoral neopterin in clinical diagnostics and prognosis

        Li Yanchun, Hu Zhidong~ Department of Microbiology and Immunology, Tianjin Medical University General Hospital, Tianjin, 300052, China
        Vol. 26, Issue 1, Pages: 45-51(2011)
        Abstract：<正>Neopterin is bioactive substance released by the activated monocyte/macrophage,and an early and sensitive marker used for reflection of cellular immune activation status induced by the lymphocyte macrophage system. Observation of neopterin levels is conducive to the diagnosis and prognosis of various diseases.This article reviews the existing evidence that neopterin has an important role in cardiovascular risk,infectious diseases,malignancy, autoimmune diseases and transplantaiton.  
          
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