Antibodies against ribosomal protein S29（RPS29）fused with glutathione’s transferase specially react with native RPS29 in mouse and human cells

Liu Jia~1; Han Junfeng~2; Li Dongying~3; Jian Rui~4; Rao XianCai~1; Chen Wei~1; Wang Jiali~1; Xu Xiaofeng~5; Hu Zhen~1; Zhang Junlei~(1) 1 Department of Microbiology; College of Basic Medical Sciences; Third Military Medical University; Chongqing 400038; China 2 Institute of Immunology; College of Basic Medical Sciences; Third Military Medical University; Chongqing 400038; China 3 Weifang Center for Disease Control and Prevention; Weifang 261041; China 4 Department of Pathogenic Biology; College of Basic Medical Sciences; Third Military Medical University; Chongqing 400038; China Department of Medical Genetics; College of Basic Medical Sciences; Third Military Medical University; Chongqing 400038; China

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Antibodies against ribosomal protein S29（RPS29）fused with glutathione’s transferase specially react with native RPS29 in mouse and human cells

Updated：2026-03-12

- Antibodies against ribosomal protein S29（RPS29）fused with glutathione’s transferase specially react with native RPS29 in mouse and human cells
- Military Medical Research Vol. 26, Issue 1, Pages: 21-28(2011)
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  CLC： R346
- Published：2011
- Accepted：
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[1].Antibodies against ribosomal protein S29(RPS29)fused with glutathione's transferase specially react with native RPS29 in mouse and human cells[J].Journal of Medical Colleges of PLA,2011,26(01):21-28.
DOI：

[1].Antibodies against ribosomal protein S29(RPS29)fused with glutathione's transferase specially react with native RPS29 in mouse and human cells[J].Journal of Medical Colleges of PLA,2011,26(01):21-28. DOI：

摘要

Abstract

<正>The ribosomal protein S29 also known as RPS29

is not only a component of the 40S subunit of ribosome

but also involved in embryonic development

oncogenesis and other pathologic conditions.However

rare commercial antibody against RPS29 restricts the discovery of precise physiological and pathological function of this protein.In this study

the whole RPS29 gene was inserted into plasmid pGEX-6p-l to express glutathione’s transferase（GST） fusion proteins in Escherichia coli（E.coli） strain BL21.High yields of soluble recombinant proteins were obtained.Mice were immunized with the recombinant RPS29 protein.The serum from the immunized mice could specially react with purified recombinant RPS29 proteins and native RPS29 proteins in CCE cells by western blotting

immunofluorescence staining and flow cytometric analysis.Further more the polyclonal antibodies also reacted specifically with human cell strain ECV304

which showed typical cytoplasmatic fluorescence.The polyclonal antibodies we prepared would be an available tool for studying the roles of RPS29 in embryonic development and human diseases.

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