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Volume 26 ， Issue 2 ， 2011
- Abstract：Objective:To determine the effects of a recombinant replication-deficient adenovirus encoding human tissue inhibitor of metalloproteinase-4（Ad.TIMP-4） on vascular smooth muscle cell（VSMC） function in vitro and neointimal development in the injured rat carotid artery.Methods:Western blotting,gelatin zymography and reverse zymography were used to characterize the expression and functional activity of the TIMP-4 secreted by Ad.TIMP-4-infected VSMCs.The migration and proliferation of VSMCs in vitro were separately detected by using Millicell-PCF invasion chambers and [3H]-thymidine incorporation assay.Immunohistochemistry and morphometric analysis were used to determine the local expression of TIMP-4 and its effect on neointima development in a rat carotid artery balloon injury model.Results:VSMCs infected with Ad.TIMP-4 expressed functionally active human TIMP-4 which increased with the duration of infection.TIMP-4 expression inhibited VSMC migration,but not significantly affect VSMC proliferation.In a balloon-injured rat carotid artery model,a significant 62% reduction in neointimal area was found in Ad.TIMP-4-infected vessels at 14 days after injury.Ad.TIMP-4 infection had no effect on medial area.Conclusion:Our results indicated TIMP-4 over expression can significantly inhibit the migration of cultured VSMCs and prevent neointimal formation after vascular injury.Our findings provide additional evidence that TIMP-4 could play an important role in vascular pathophysiology,and may be an important therapeutic target for future drug development.0|15|0Updated：2026-03-12
- Abstract：Background:The vitamin D receptor（VDR） gene is a primary candidate gene for tuberculosis susceptibility,but results of previous studies are somewhat contradictory and underpowered.Thus,it is essential to further explore the association between VDR gene polymorphisms and risk of pulmonary tuberculosis（PTB）.Methods:A systematic review and meta-analysis about the association between FokI,TaqI,ApaI and BsmI polymorphisms and PTB susceptibility was conducted.Statistical Package for Social Science（Version 13.0） and Review Manager（Version 4.2,The Cochrane Collaboration） were used to analyze the data reported in studies.Results:A total of 13 studies with 2 262 cases and 2 833 controls were involved in the FokI polymorphism,and the results showed FokI polymorphism was associated with PTB susceptibility（allele f vs F:OR=1.12,95% CI=[1.02,1.23];the additive effect model ff vs FF:OR=1.40,95%CI=[1.10,1.77];the recessive genetic model ff vs Ff+FF:OR=1.39,95%CI=[1.12,1.71]）.No significant associations were observed between TaqI（15 studies with 3 031 cases and 3 132 controls）,ApaI（7 studies with 1 495 cases and 1 922 controls）,BsmI（6 studies with 919 cases and 1 250 controls） variants and PTB susceptibility.Conclusion:We found variant FokI polymorphism of VDR gene may play a risky role in PTB development,and the genetic model was presumed to be recessive.0|42|2Updated：2026-03-12
- Abstract：Objective:To study the epidemiological features of pancreatic cystadenoma and cystadenocarcinoma in China during the last 10 years and to analyze the diagnosis and treatment.Methods:Reports on pancreatic cystadenoma and cystadenocarcinoma published from 2000 to 2009 were retrieved from various databases,such as WANFANG data,VIP web and China National Knowledge Infrastructure（CNKI）.The epidemiological features of pancreatic cystadenoma and cystadenocarcinoma and its diagnosis and treatment were analyzed.Results:Totally 1 865 patients with pancreatic cystadenoma and cystadenocarcinoma were reported in China during the last 10 years.The male to female ratio was approximately 1:2.1.The accurate ages were reported in 1 536 cases,the average age of them was 50.8 years,whose average age from 40 to 60 years old accounted 75.9% of the patients.65.3% of the cases were located in East China and Central China.Abdominal pain was the main clinical manifestation and was found in 54.9% of the patients.Pancreatoduodenectomy and resection of body and tail of the pancreas were the main procedure for the treatment of pancreatic cystadenoma and cystadenocarcinoma.Conclusion:Pancreatic cystadenoma and cystadenocarcinoma were mainly found in older women in East and Central China.Preoperative diagnosis is difficult.Pancreatoduodenectomy and resection of pancreatic body and tail were the main procedure for the treatment of pancreatic cystadenoma and cystadenocarcinoma.0|19|0Updated：2026-03-12
- Abstract：Objective:To investigate the association of XRCC4 polymorphic variants at G-1394T（rs6869366） with colorectal cancer susceptibility.Methods:In this hospital-based case-control study,the association of XRCC4 polymorphism with colorectal cancer risk in Chinese population was investigated.In total,171 patients with colorectal cancer and 171 healthy individuals matched for age and gender were selected.The genomic DNAs of the patients and controls were extracted from peripheral blood and the 300 bp target DNA was amplified with Polymerase Chain Reaction.The products were then digested with restriction endonuclease HincII,followed by agarose electrophoresis to identify the genotype.Results:We found a significant difference in the frequency of the XRCC4 G-1394T genotype between the colorectal cancer and control groups in female（1/127 vs 8/122,P<0.05）.Those with G/T at XRCC4 G-1394T showed a decreased risk of colorectal cancer susceptibility compared with those with T/T（OR 0.113,95%CI 0.014-0.932）.However,in overall population or in male,there was no significant difference of the distribution between the colorectal cancer and control groups.Conclusion:Our findings suggested that the G allele of XRCC4 G-1394T were associated with decreased risk of colorectal cancer susceptibility in female.0|26|1Updated：2026-03-12
- Abstract：Oxidative stress plays critical role in the pathogenesis of chronic obstructive pulmonary disease（COPD）,so therapy targeted on oxidative stress is of great importance for COPD.However,since the current used antioxidants are not satisfying,scientists have to find more effective antioxidants for COPD.Hydrogen,the most abundant chemical element in the universe,was recently discovered as a novel therapeutic medical gas,and has shown great antioxidant potential in a series of recent published researches for its feature of selectively reducing toxic reactive oxygen species.Based on these findings,we hypothesize that hydrogen gas therapy might be a novel,effective,safe,and economic treatment for COPD patients in one day.0|19|0Updated：2026-03-12
- Abstract：Forensic medical workers are faced with unique ethical challenges in their attempt to deliver medical care in a custodial environment.We first figure out the reasons why the doctor is standing opposed to the patient through questionnaire and interview of the prison doctor in Shanghai,China,which turn to be a bilateral issue.Both sides should take more active approaches and put themselves to the right position to contribute to a better medical environment in the prison hospital,which can reduce medical tangles and help the criminals to accomplish their reform process.0|33|0Updated：2026-03-12
- Abstract：Delivering bad news regarding patient’s health is an unavoidable task for physicians and medical students in their careers.This is especially difficult for beginners.Disclosing undesired medical information to patients is technically and mentally demanding.Only with proper communication skills and sufficient patient-contact practice can medical practitioners smoothly cope with this stressful mission.There are a number of common mistakes we fall into when delivering bad news,which might induce or exacerbate patients’ anxiety.Sometimes we are prone to disclose medical information in a "straight-to-the-point" manner.This is often a futile attempt made on the physician’s part to ease his or her own uncomfortableness when delivering bad news,but unfortunately it does no good to facilitate patients’ acceptance of the actual condition.Instead of using medical jargons,physicians are obliged to use "layman’s terms" that make better sense to their patients.Declaring a clinical diagnosis without corresponding explanation on the prognosis and the discussion of treatment can also bring much confusion and anxiety to patients.Another even worse situation might be that we finish the "lecture" in our own words without confirming the patient’s comprehension and addressing their questions.Professionals have recommended a six-step protocol on delivering bad news to patients:"SPIKES":S-Setting up the interview;P-assessing the patient’s Perception;I-obtaining the patient’s Invitation;K-giving Knowledge and information to the patient;E-addressing the patient’s Emotions with Empathic responses;S-Strategy and Summary.0|24|1Updated：2026-03-12
- Abstract：Teaching tools available on the internet presented in this paper may help the students in the study of cancer prevention to understand the consequences of lifestyle on health.They are all in English,they have been downloaded for free and consist mainly in scientific texts and other documents,tables,graphics,atlases and animations.0|10|0Updated：2026-03-12
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