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Volume ， Issue 2 ， 2006
- Abstract：<正>Objective: To investigate the mechanism of oxidative stress in rotenone neurotoxicity to dopaminergic neuron PC12. Methods: High differentiated PC12 cells as dopaminergic neurons were treated by different concentrations of rotenone. The morphology was observed with inverted phase contrast microscope and transmission electron microscope. Cell viability and proliferation inhibition were assessed by MTT. SOD and MDA were detected with biochemical assay. And the specific fluorescent probe （DCF-DA） was used to examine ROS in PC12 cells. Results: After treated with rotenone for 24 h, most of the PC12 cells became smaller and rounder. The process of axon was reduced, shortened or broken in a time and concentration dependent manner. The mitochondrial structure and metabolism were changed. Endoplasmic reticulum expanded and the free ribosome increased. Compared with the control group, cell proliferation inhibition increased and cell viability decreased. SOD increased and MDA decreased. The intensity of fluorescence was more obvious in PC12 cells treated by rotenone compared with control group. Conclusion : Rotenone is neurotoxic to cultured dopaminergic neuron PC12. Rotenone might exert this effect through the metabolism of oxidative stress on the pathogenesis of the neuron.0|25|1Updated：2026-03-12
- Abstract：<正>Objective: To investigate the role of peroxisome proliferator-activated receptors δ （PPARδ） in inflammatory reaction and its possible mechanism in adipocyte. Methods :Lentivirus-mediated RNA interference （RNAi） was used to block the expression of PPARS in 3T3-L1 cells. In order to induce inflammation in 3T3-L1, cells were stimulated with tumor necrosis factor-α（TNFα, 20 ng/ml） for 4 h. The expression of PPAR8, nuclear factor κB （NFκB） and C reactive protein （CRP） were determined by Western blot analysis. Results:The expression of PPARδ was reduced by 80% after RNAi. Blockage of PPARδ promoted the expression of CRP and NFκB in cells stimulated with TNFα, but had no effect on normal cells. Conclusion: PPARδ is involved in inflammatory reaction in adipocyte. Blockage of PPARδ can promote the inflammation mediated by inflammatory factors and increase the expression of NFκB and CRP in 3T3-L1 cells stimulated with TNFα.0|47|2Updated：2026-03-12
- Abstract：<正>Objective: To obtain high quality of mitochondrial DNA （mtDNA） and carry out extra-long PCR （XL-PCR）. Methods: Mitochondria were isolated by differential centrifugation, and membranes were disrupted using 10%SDS （pH 7. 0）. mtDNA was then extracted using phenol and chloroform. Results: The mtDNA obtained by using our improved method can be used as effective template for XL-PCR, and total mtDNA （16 kb） can be amplified easily. Conclusion: Our improved method is effective in preparing high quality of mtDNA, which can be used as template for XL-PCR.0|21|0Updated：2026-03-12
- Abstract：<正>Objective:To evaluate the probability and efficacy of endostatin-vascular endothelial growth inhibitor （VEGI） recombinant adenoviruses combined with dexamethasone on suppressing heterolamellar corneal transplantation rejection. Methods: Heterolamellar corneal transplantation models were established in 64 New Zealand rabbits, which were randomized into 4 groups of 16 rabbits each. After the transplantation, all the 64 right eyes were injected subconjunctively with 0. 2 ml saline （saline group）, 0. 1 ml AdCA13-hENDO-VEGI151 plus 0. 1 ml saline （AdCA13-hENDO-VEGI151 group）, 0. 1 ml Dexamethasone （DXM） plus 0. 1 ml saline （DXM group）, 0. 1 ml AdCA13-hENDO-VEGI151 plus 0. 1 ml DXM （Ad-CA13-hENDO-VEGI151 combined with DXM group） with one time each 3 days for 10 times. Graft survival and ocular surface were observed for 6 weeks. The fusion protein expression was detected by immunohistochemistry 6 weeks after transplantation. Results: Both the CNV index, rejection index and the xenograft rejection rate in the AdCA13-hENDO-VEGI151 combined with DXM group were statistically lower than those in other groups. AdCA13-hENDO-VEGI151 combined with DXM group: 1. 375 0±0. 500 0, 2. 750 0 ±1. 843 9 and 6. 25% respectively 6 week after keratoplasty; Saline group: 3. 437 5±0. 512 3, 8. 812 5± 1.1087, 100.00%; AdCAl3-hENDO-VEGI151 group: 2. 312 5±0. 478 7, 5. 625 0±0. 957 4, 62.50%; DXM group: 3. 000 0±0. 816 5, 5. 562 5±1. 315 0, 56.25% （P<0. 01）. Immunohistochemical staining showed the fusion protein expressed mainly in corneal epithelium. Conclusion: The fusion protein expressed by the recombinant adenovirus has significant effect on inhibiting neovascularization after heterolamellar corneal transplantation. The topical application of AdCA13-hENDO-VEGI151 combined with DXM suppressed effectively the postoperative xenograft rejection rate of heterolamellar corneal transplantation.0|13|2Updated：2026-03-12
- Abstract：<正>Objective: To investigate the effects of basic transcriptional element binding protein-2 （BTEB2） antisense RNA on vascular smooth muscle cells （VSMCs） proliferation and the neointimal formation after carotid balloon injury in rats. Methods: The cultured VSMCs were transfected with an adenoviral vector containing BTEB2 antisense gene, Ad ASBTEB2. Effects of BTEB2 antisense RNA on the expression of BTEB2 were investigated by Western blot analysis. The cell cycle was analyzed using flow cytometry. Ad ASBTEB2, control adenoviral vector Ad. LacZ or PBS was transduced into the rat carotid artery after balloon injury. The expression of BTEB2 at 7, 14, and 21 d after gene transfer was detected by immunohistochemistry and neointima-to-media （I/M） area ratio at these time points was calculated. Results: The cell cycle was arrested in G0/G1 phase and the expression of BETB2 was downregulated after transfection with Ad ASBTEB2. Ad ASBTEB2 treatment reduced I/M area ratios on day 7, 14, and 21 after injury by 45%, 50% and 53% respectively, whereas the Ad LacZ treatment did not significantly alter these ratios compared with control group. Conclusion: BTEB2 antisense RNA mediated by adenoviral vector inhibits proliferation of VSMCs and significantly reduces neointimal hyperplasia in the rat carotid balloon injury model. BTEB2 antisense RNA is a potential therapeutic approach to preventing neointimal formation after balloon injury.0|26|0Updated：2026-03-12
- Abstract：<正>Objective: To improve DC-based tumor vaccination, we studied whether dendritic cells （DCs） which cocultured with H22 liver cancer cells-derived heat shock protein （HSP） glycoprotein 96 （gp96） affect the T cell-activating potential in vitro and the induction of tumor immunity in vivo. Methods: Maturation of murine bone marrow-derived DC was induced by GM-CSF plus IL-4. which mimiced the immunostimulatory effect of DC. Cocultured DC and gp96-peptide complexes were used to vaccine H22 liver cancer cells of mice. Using murine models we compared the immunogenecity of DC modified by gp96-peptides complexes derived from murine liver cancer cells alone or inactive tumor cells. To verify the specificity of the vaccine, in vitro assays were executed. Serum cytokine levels were quantified to explore the supposed pathway of DC modified by gp96 peptide complexes and its effect on antitumor immune response. Results: DC modified by gp96-peptide complexes can activate spleen lymphocyte and the latter can specifically kill H22 cells but not Ehrilich ascites carcinoma cells. Modified DC can induce potent tumor-antigenspecific immune response, augment the proliferation of Th1 cells, and inhibit tumor growth. Conclusion: In this study, we have developed a novel DC-mediated tumor vaccine by combing the gp96 antigenic peptides complexes and inducing immune response against specific tumor cells. gp96 can be identified as a potent DC activator.0|53|0Updated：2026-03-12
- Abstract：<正>Objective:To investigate the production of nitric oxide（NO） and the expression of indurible nitric oxide synthase （iNOS）. and their possible role in abdominal aortic aneurysm （AAA）. Methods: A total of 28 patients with AAA, 10 healthy controls, and 8 patients with arterial occlusive disease were enrolled into this study. Standard colorimetric assay was used to examine NO concentration in plasma from patients with AAA and normal controls, and in cultured smooth muscle cells （SMCs）. Expression of iNOS in aortas and cultured SMCs were detected by immunochemistry. The correlation of iNOS expression with age of the patient, size of aneurysm, and degree of inflammation was also investigated by Cochran-Mantel-Haenszel X2 test and Kendall correlation. Results: Expression of iNOS increased significantly in the wall of aneurism in the patients with AAA compared to the healthy controls （P<0. 05） and the patients with occlusive arteries （P<0. 05）. iNOS protein and media NOx （nitrite + nitrate） also increased in cultured SMCs from human AAA （n = 4, P<0. 05）, while plasma NOx decreased in patients with AAA （n = 25） compared to the healthy controls （n = 20）. There was a positive correlation between iNOS protein and the degree of inflammation in aneurismal wall （Kendall coefficient = 0. 5032, P = 0. 0029）. Conclusion: SMCs and inflammatory cells are main cellular sources of increased iNOS in AAA, and NO may play a part in pathogenesis in AAA through inflammation, SMCs and oxidative stress.0|28|1Updated：2026-03-12
- Abstract：<正>Objective: To explore the pathogenesis of avascular necrosis of femoral head （ANFH）, the early diagnosis index and the treatment effective index of ANFH in clinical practice. Methods: Twenty-four Japanese rabbits were divided into 2 groups: model group and control group. ANFH models were produced by intramuscular injection of large dosage of steroid to rabbits for 8 weeks. On the 4 th, 8 th week after the injection, two rabbits each time from each group were taken to observe the structure of femoral head by light microscope and scanning electron microscope. Four other stomach-empty rabbits from each group were also used to test the contents of Nitric Oxide （NO）, contents of the hemorheology indexes. Results: Compared with the control group, the rabbits in the model group exhibited osteoporosis of femoral head and more bone lacuna and more fat cells through light microscope. Through scanning electron microscope observation bone trabecula were broken and sunk, and collagen fibers on the surface of bone matrix became loosen and broken, more osteocyte had pyknosis, adipocyte in the medullary cavity were enlarged and subchondral arterioles and capillaries of the femoral head were pressed by adipocyte. Compared with the control group, the model rabbits contained less NO and obvious increase of the plasma viscosity （PV）, low blood viscosity （LBV）, erythrocyte hematocrit （HCT）, indices of erythrocyte rigidity （TK） and indices of erythrocyte aggregation （AI）, plasma fibrin level （PFL） （P<0. 01） and an increase of erythrocyte electrophoresis rate （ERT） （P< 0.05）. High blood viscosity （HBV）, and erythrocyte sedimentation rate （ESR） were unchanged. Conclusion: The steroid-induced ANFH might be related to less NO and the abnormal hemorheology; and NO and hemorheology should be considered as an early diagnosis index for ANFH in clinical practice.0|79|2Updated：2026-03-12
- Abstract：<正>Objective:To compare the phenotype characteristics of rat annulus fibrosus （AF） cells cultured on flexible silicone membranes and those in plastic plates. Methods:The morphology of AF cells cultured in different substrates was examined. Proteoglycan was stained by toluidine blue. Contents of collagen type I , collagen type II and aggrecan mRNAs were determined by reverse transcription-polymerase chain reaction （RT-PCR）. The expression of integrin β1 was monitored by flow cytometry. By using propidium iodide （PI）, the cell cycle in AF cells was analyzed. Cell adhesion to silicone membrane was also measured. Results:The AF cells cultured on different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on these 2 substrates. They still had the same expression levels of collagen type I , collagen type II , aggrecan mRNAs, and integrin β1. No significant difference was observed in the distribution of the cell cycle. AF cells grew well on silicone membrane. Conclusion: AF cells cultured on flexible silicone membrane maintain the stability of phenotype and may be appropriate for further studying the metabolic responses to mechanical stimuli at the cellular level.0|17|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the altered expression of lipid metabolism-related gene adipose differentiation-related protein （ADRP） in pre-eclampsia. Methods: Semi-quantitative RT-PCR and Western blotting were used to validate the altered expression of ADRP gene between pre-eclamptic placentas （pre-eclampsia group） and normotensive placentas （control group） respectively. In situ hybridization （ISH） was used to localize ADRP mRNA in pre-eclamptic placentas. Results: There was a significant difference in the levels of placental ADRP mRNA between pre-eclampsia group and control group （1. 98±0. 50 vs 1. 09±0. 20, P<0. 01）. Western blotting showed that placentas both in pre-eclampsia group and control group expressed the special ADRP band at 48. 1 kD. The relative levels of ADRP protein in pre-eclampsia group were significantly higher than those of control group （0. 40 ±0. 19 vs 0. 19 ±0. 09, P<0. 01 ）. ADRP mRNA was diffusely distributed in pre-eclamptic placentas. Their positive staining existed in cytoplasm of trophoblast. Conclusion: Abnormal expression of ADRP gene in pre-eclamptic placenta may be associated with the pathogenesis of pre-eclampsia.0|24|0Updated：2026-03-12
- Abstract：<正>Objective:To explore the protective effect of remifentanil on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury and their possible mechanism. Methods:The model of rat hepatic ischemia-reperfusion injury was used and the effect of remifentanil on the ultrastructure of mitochondria, calcium homeostasis, MDA level in mitochondria were observed. Results: In contrast with the control group, mitochondrial matrix calcium concentration, calcium concentration after calcium uptake, and the quantity of calcium uptake in low and high remifentanil concentration groups and 5-HD group are lower （P<0. 01）, and there is no difference in RHD （5-HD+remifentanil） group. The difference in MDA level between groups is insignificant. Conclusion:Remifentanil at clinical concentrations exerts a protective effect on mitochondria in rat hepatocytes subjected to ischemia-reperfusion injury, in which activating the KATP channel may be involved.0|30|5Updated：2026-03-12
- Abstract：<正>Objective: To compare function recovery of left ventricle after off-pump and on-pump coronary artery bypass （OPCAB and ONCAB） using Tei index. Methods: Twenty-four patients with coronary artery disease were enrolled, twelve of which received OPCAB and the others underwent ONCAB. Left ventricular ejection fraction （LVEF）, E/A ratio at mitral orifice and Tei index were measured using transthoracic echocardiography before surgery and 3-7 days, 1 month, 3 months and 6 months after surgery. Results:Tei index of both groups decreased 3-7 days after surgery, with OPCAB group’s lower than ONCAB group’s. The difference between pre-and post-OPCAB was significant （P<0. 01 ）, but not for ONCAB group （P>0. 05）. Tei index of ONCAB decreased more significant than that of OPCAB 1 month after surgery, there was statistical difference between 3-7 days and 1 month after surgery in ONCAB （P<0. 01）. Afterwards, Tei index of the 2 groups decreased steadily with no significant difference between them at other time points. LVEF and E/A ratio decreased at first, then increased gradually, with no statistical differences between the 2 groups at all time points. Conclusion: The recovery of left ventricular function after OPCAB is earlier than ONCAB. Tei index is more sensitive than LVEF and E/A ratio in detecting cardiac function recoveries and can be considered as an accurate and simple method to evaluate left ventricular systolic and diastolic function.0|6|0Updated：2026-03-12
- Abstract：<正>Objective: To evaluate the clinical value of laparoscopic inguinal hernia repair in hernioplasty and simultaneous cholecystectomy. Methods: Twenty-eight patients with symptomatic chronic calculous cholecystitis and synchronous unilateral primary inguinal hernia were performed combined surgery between October 2001 and March 2005. Of them, 10 cases underwent laparoscopic totally extraperiloneal mesh hernia repair （TEP） and laparoscopic cholecystectomy （LC）, 3 cases underwent laparoscopic transabdominal preperitoneal mesh hernia repair （TAPP） and LC, and 15 cases underwent LC and open tension free hernia repair. Results: All the procedures were performed successfully, 2 patients occurred urinary retention in LC + open group and 1 patient occurred scrotum seroma in LC + TEP procedures. During the 6 to 24 months’ follow-up, no hernia recurrences occurred in all patients. There were 6 patients （40%） in LC + open group had discomfort pain in the inguinal region and lasted 1 to 3 months. The operating time was longer in the totally laparoscopic group （TEP + LC and TAPP + LC） （104±31 min） than in the LC+open group （80±28 min） （P<0. 05）. The intensity of postoperative pain at rest was greater in the LC + open group at 24 h （P<0. 05） and 48 h （P<0. 05）. No differences between the 2 groups were found in the mean operating costs and oral intake of the postoperative period. But the time resume to walking （2. 9 vs 1. 8 d） （P<0. 01） and the mean hospital stay （8. 2 vs 4. 6 d） （P<0. 001） was longer in the LC + open group than in the totally laparoscopic group. Conclusion: In the same operating costs, the totally laparoscopic precedure has more advantages of low postoperative pain, quicker resume to walking and less hospital stay than open tension-free hernia repair in hernioplasty and simultaneous LC. Thus, the totally laparoscopic approach is considered to be advantage of the hernioplasty and simultaneous LC.0|14|1Updated：2026-03-12
- Abstract：<正>Objective:To evaluate the clinical effects of the abdominal aorta block in controlling haemorrhage during operations of the gynecologic tumor. Methods: From July 1965 to January 2005. we collected patients （n = 49） of gynecologic tumor complicated with haemorrhage during operations, who were divided into 3 groups: preventive blocking group （PG, n=12）, treatment blocking group （TG, n = 20） used abdominal aorta block technique with sterilized cotton band and silica gel tube, and control group （CG, n = 17） which were used the regular haemostatic methods, such as ligature, suture and ribbon gauze packing. During operations, the vital signs including the amount of bleeding and transfusion were measured. Results: Compared with the CG, the amount of bleeding and transfusion in the PG and TG decreased significantly （P<0. 01）. After using the technique, 32 cases of haemorrhage were controlled completely. All patients finished operation smoothly in the end and the vital signs were stable. The vision field of operation was clear and the operating time was shortened dramatically （3. 0 h vs 5. 7 h and 3. 8 h vs 5. 7 h, P< 0. 01）. No complications caused by the block occurred in the post-operation. Conclusion: Lower abdominal aorta block is safe and effective in controlling haemorrhage during operations of the gynecologic tumor.0|20|0Updated：2026-03-12
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