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Volume ， Issue 1 ， 2006
- Keywords：cisplatin;sound preconditioning;cochlear;nitric oxide;inducible nitric oxide synthase0|16|0Updated：2026-03-12
- Keywords：dermal papilla cells;HSPC016;transcriptional regulation0|16|0Updated：2026-03-12
- Abstract：<正>Objective: To establish a rabbit atrial fibrillation model with rapid atrial pacing （RAP） and investigate its ultrastructural changes and expressions of L-type calcium channel subunits and potassium channel Kv4. 3. Methods: Thirty-six rabbits were performed electrical stimulation through bipolar endo-cardial led by surgical technique. 600 beat per min from 0 to 48 h. Atrial ultrastructure was observed by transmission electron microscope （TEM） after different pacing times. mRNA were measured by reverse transcription-polymera.se chain reaction （RT-PCR）. Results: Atrial ultrastructure had alteration after 3 hours’ pacing, such as mitochondria vacuolization, myofilament lysis and glucogen aggregation. The mRNA of the Ca2+ channel β1 and α1 subunits began to decrease after pacing of 6 h. which were paralleled with the change of Kv4. 3 mRNA. But the auxiliary subunit α2 were not affected. Conclusion: Ultrastructural changes and mRNA levels of L-type calcium channel subunits and potassium channel Kv4. 3 are decreased after RAP. with a mechanism of transcriptional down-regulation of underlying ion channels due to calcium overloading after RAP.0|13|0Updated：2026-03-12
- Abstract：<正>Objective: To study induction of apoptosis and inhibition of proliferation in Hep-2 by antisense survivin RNA. Methods: Antisense survivin RNA expression vector was constructed and then was transfected to human laryngeal carcinoma cell line Hep-2 by lipofectamine. HpEGFP/survivin cells （transfected with the combinant of antisense survivin RNA） were obstained by using G418. The levels of survivin protein before and after transfection were determined by Western-blot. Proliferation activity was measured by MTT assay. The experiment of colony formation in soft agar was carried out for assessing ability of proliferation of Hep-2 cell. Apoptosis was assessed by flow cytometry and acrdine orange（AO）. Results:After antisense survivin RNA plasmids were transfected, the level of survivin protein was inhibited in Hep-2. Compared with control, proliferation of HpEGFP/survivin cells were suppressed significantly. The experiment of colony formation in soft agar showed the ability of colony formation decreased in HpEGFP/survivin cells compared to control （P<0. 05）. Apoptosis rate increased about 1. 81 folds compared with control. Conclusion: The antisense survivin RNA can partly inhibit the level of survivin protein expression in Hep-2 and can induce apoptosis and inhibit the proliferation of Hep-2 by down-regulating the expression of endogenous survivin in vitro.0|31|0Updated：2026-03-12
- Abstract：<正>Objective: To identify the plants in Vitex including Vitex negundo L. , V. negundo var. cannabifolia （Sieb. et Zucc. ） Hand. Mazz. , V. trifolia L. and V. trifolia L. var. simplicifolia Cham. . Methods: Both intra- and inter-species relationships among these plants were analyzed by RAPD marker. Twenty-one samples collected from different locations in China were tested using 25 RAPD arbitrary 10mer primers which were screened from 32 primers, Cluster analysis was conducted by Ward’s minimumvariance method of SAS software. Results: A total of 224 bands were produced and 128 bands showed polymorphism among 21 samples. The level of polymorphism within species was 51. 7%. The dendrogram constructed based on RAPD analysis showed that 21 samples can be placed in 2 groups at the level of SPRS value of 0. 3636. The first group included V. trifolia var. simplicifolia and V. trifolia. The other consisted of V. negundo and V. negundo var. cannabijolia. . At the level of SPRS value of 0. 1, 21 samples can be obviously divided into 3 groups. V. trifolia and V. trifolia var. simplicifolia were clustered into one group. V. negundo and V. negundo var. cannabifolia were separately clustered to 2 groups. Conclusion: RAPD analysis is in good agreement with the traditional plant taxonomic classification, and fundamentally identical with their origins and morphologic characteristics, which indicates that the genetic relationship a-mong samples is related to their center of origin. These medicinal species in Vitex have their specific bands which are available to identify. These specific bands can be used as species-specific molecular markers of Fructus Viticis for the application of germplasm identification and classification.0|65|0Updated：2026-03-12
- Abstract：<正>Objective: To explore the effect of high dose of glucocorticoid (GO on the synthesis of corticotropin releasing hormone （CRH） and transcription of its mRNA in hypothalamus paraventricular nuclei （PVN） in order to investigate its difference with that of traditional GC effects and to add a new possible explanation to the mechanism of clinical applications of high dose of GC. Methods: A total of 60 rats were divided into 5 groups; blank control. 10’’ mol/L dexamethasone （DEX） group. 10-9 mol/L DEX group, 0. 9% saline group and GR blocking group （102mol/L RU486）. All agents were administrated through the femoral vein. CRH protein expression was measured by immunohistochemistry and laser confocal scanning microscopy （LCSM）; CRH mRNA level was explored by in situ hybridization. Results: 10-6 mol/L DEX made CRH mRNA transcripted after 20 min and its protein expressed in PVN after 30 min, while normal level of DEX and 0. 9% saline could not. If GR was blocked in advance, the effect of high dose of DEX disappeared. Conclusion; High dose of GC can have CRH increased in PVN, which differs to the effect of traditional GC. And mGRH may play an important role in the effect of high dose of GC but not classic iGR.0|26|1Updated：2026-03-12
- Abstract：<正>Objective: Phase-contrast X-ray imaging which reduces radiation exposure, is a promising technique for observing the inner structures of biological soft tissues without the aid of contrast agents. The present study intends to depict blood vessels of rabbits and human livers with hard X-ray in-line outline imaging without contrast agents using synchrotron radiation. Methods: All samples were fixed with formalin and sliced into 6 mm sections. The imaging experiments were performed with Fuji-IX80 films on the 4W1A light beam of the first generation synchrotron radiation in Beijing, China. The device of the experiment, which supplies a maximum light spot size of 20×10 mm was similar to that of in-line holography. The photon energy was set at 8 KeV and high quality imagines were obtained by altering the distance between the sample and the film. Results: The trees of rabbit-liver blood vessels and the curved vessels of the cirrhotic human liver were revealed on the images, where vessels < 20 μm in diameter were differentiated. Conclusion: These results show that the blood vessels of liver samples can be revealed by using hard X-ray in-line outline imaging with the first generation synchrotron radiation without contrast agents.0|35|0Updated：2026-03-12
- Abstract：<正>Objective: To present a self-developed experimental system for basic studies of blood perfusion imaging and time-intensity evaluating based on ultrasound contrast agent. Methods: The experimental system performed the image reconstruction and time-intensity processing with radio frequency signals. The system was comprised of ultra-high speed hardware data acquisition interface and low computational cost algorithms. The self-made contrast agent,blood mimic phantom and capillary phantom model were used to validate the experimental system. Results: The images acquired in blood phantoms with linear-array and curve-array transducers were given. The time-intensity curves corresponding to selected region of interested from image sequence were demonstrated. It was also shown the time-intensity based decay curves and a comparison of decay of ultrasound contrast agent under different ultrasound powers. Conclusion: Several experiments resulted from two in vitro phantom models show that the experimental system can be used to basic studies of blood perfusion and further clinical studies of microvasculature perfusion.0|44|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the effect of ultraviolet blood irradiation and oxygenation （UBIO） on the metabolism of oxygen free radicals in rabbits with acute soman intoxication. Methods: One hundred rabbits were randomly divided into 5 groups: normal control group, intoxication group, routine therapy group. UBIO therapy group and combined therapy group. After 14 d, the concentration of malondiadehyde（MDA） and activity of superoxide dismutase（SOD）, glutathionperoxidase（GSH-Px）, catalase （CAT） and total antioxidative capacity （T-AOC） in serum were determined respectively. Results: Compared with the normal control group, the concentration of MDA and activity of CAT in the intoxication group were significantly higher （P < 0. 05）. but SOD. GSH-Px activity and T-AOC were significantly lower （P<0. 05）. After UBIO or combined therapy, serum MDA level was significantly lower in comparison with intoxication group （P<0. 05）. but the activity of SOD. GSH-Px, CAT and T-AOC were higher than intoxication group（P<0. 05）. Conclusion: There is an obvious oxygen free radical injury in rabbits with a-cute soman intoxication. UBIO can improve the antioxidation ability of rabbits and may be applied to treat acute soman intoxication as adjunctive therapy.0|26|0Updated：2026-03-12
- Abstract：<正>Objective:To explore the effects of predegenerated peripheral nerve graft （PPNG） combined with nerve growth factor （NGF） infusion on ascending sensory tract regeneration after spinal cord injury. Methods: Fifty female SD rats were randomly divided into 5 groups. Group A was treated with PPNG and NGF infusion, group B with PPNG, group C with NGF infusion, group D and group E were blank and normal control, respectively. Horseradish peroxidase-labled （HRP） tracing method was employed to evaluate the regeneration of injured nerves after 8 weeks. The extent of regeneration in and beyond the nerve graft was determined by counting the number of HRP-labeled fibers intersecting imaginary lines perpendicular to the axis of the graft and cord. For the sake of convenience, according to the relation of the PNG and spinal cord, 6 model zones were divided, including caudal of spinal cord, caudal transition zone, caudal zone in graft, rostral zone in graft, rostral transition zone and rostral of spinal cord. Results: On the transverse section of caudal zone in graft, rostral zone in graft, rostral transition zone, the fibers in group A were significantly higher than that in group B and C （P<0. 05）. Conclusion: PPNG combined with NGF may significantly promote the regeneration of ascending long tract after spinal cord injury. The regenerative fibers can penetrate the 2 graft-host interface scars.0|55|1Updated：2026-03-12
- Abstract：<正>Objective:To observe the changing of biomechanical features during the degradation course of poly-D,L-lactic acid （PDLLA） rods in vivo and in vitro and to evaluate its value as an internal fixation material. Methods:PDLLA rods were emerged into PBS simultaneous body fluid with constant temperature of 37 C and the rods were embedded into muscle tissue of 20 rabbits for degradation in vitro and in vivo . The rods were taken out in 2, 4, 6. 8 and 12 weeks. Biomechanical features of bending, shearing and axial compression strength, rigidity and elastic modulus were observed during the degradation course. Statistical method was used to test the changes of biomechanical parameters. Results: （1） There was similar changes of bending, compressive, shearing strength and bending, compressive and shearing rigidity of the PDLLA rods between in vivo and in vitro. （2）Bending, compressive, shearing strength decreased 33%, 18% and 43% respectively within the first stage of the degradation, and after 6 weeks of degradation, they decreased slowly. （3）Elastic modulus, bending, compressive and shearing rigidity.decreased sharply during the 6 weeks of degradation, with a drop of 22% , 39% and 30% respectively, and after 8 weeks, they decreased slowly. Even after 12 weeks of degradation, the strength of the rods was still higher than that of sponge bone. Conclusion: During the degradation of the material, the strength and rigidity of PDLLA rods can meet the need of fracture fixation of cancellous bones.0|29|1Updated：2026-03-12
- Abstract：<正>Objective: To explore the molecular biological mechanism of Arnebia root oil promoting wound surface healing by observing histological change and bFGF expression in wound surface tissue as well as wound surface healing rate. Methods: Raw surface in patients were randomly divided into 2 groups. Experimental group was treated by Arnebia root oil and control group was treated by petrolatum gauze, then the tissular structure of raw surface was observed by histology, histochemistry. electron microscope and raw surface healing rates was compared either. bFGF expression in wound surface tissue was also evaluated by Western-blot. Results: Raw surface healing rate of experimental group and control group had obvious difference（P<0. 05）. Raw surface of experimental group had more fibroblast, collagen and blood capillary. bFGF was expressed in both groups, and the level of bFGF expression in experimental group was higher than that in control group in every period. There were significant differences between 2 groups in gray-density value （ P<0. 05）. Being as an internal control, no significant change was found for β-actin expression, although it occured in various phases. Conclusion: Arnebia root oil plays an important regulative role in the course of healing of wound and it can promote skin raw surface repair and accelerate wound surface healing, which are caused by enhancing bFGF in the wound tissue.0|52|1Updated：2026-03-12
- Abstract：<正>Objective: To review the evolution of fluid therapy （IOFT） during liver transplantation （LTX） based on clinical experience in our institute over 7 years. Methods: All patient records （n = 130） of LTX from 1996 to 2003 were examined. After excluding patients with co-morbidities 100 cases were found suitable for IOFT analysis. All patients had undergone LTX and follow-up under the same surgical team. Based on IOFT records we tried to identify distinct patterns of practice evolving over 7 years. Intraoperalive hemodynamics （IOHD） and long-term outcome records were examined. Results: Retrospectively, 3 types of IOFT were found. Group A （n= 18, period 1996-1997） received high amounts of crystalloids; group B （n= 24, period 1998-2000） received high amounts of plasma and albumin; and group C （n = 58, period 2001-2003） received lower amounts of albumin and plasma and recommended amounts of 6% hy-droxyethyl starch 200/0. 5 （HES） and high amounts of vasoprcssors. Intraoperatively, group A exhibited the highest levels of central venous and pulmonary artery pressures in the neo-hepatic stage （P<0. 05）. Postoperatively, the patients in group C had the shortest time to extubation; the values for group A,B,C were （15.8±11）, （17. 3±10. 2） and （7. 98±3. 2） h respectively（ P<0. 05）. At the end of one-year follow-up, the patients in group C had the lowest mortality （group A, B, C were 27. 78%, 29. 17% and 6. 25% respectively; P<0. 05）. Conclusion: In our institute over the years the use of crystalloids, albumin and plasma during IOFT of LTX is gradually replaced to a large extent by HES. The improvements in IOHD and long term outcomes are likely to be related to improved surgical experience of our team. Nevertheless, the shift in IOFT practices might be associated with an beneficial effect on IOHD or long term outcome. Treatment with proper amount of liquid and vasoactive drugs may be a better method of fluid therapy.0|13|0Updated：2026-03-12
- Abstract：<正>Objective: To sum up 7 patients with cervicothoracic vertebrae tumors （C7 to T3） from March 1999 and May 2002. who underwent operative treatment via anterior approaches. Methods:The anterior approaches included low anterior cervical approach and high transthoracic approach. In 5 cases of segments of T1 and above involved, the low anterior cervical approaches were adopted, otherwise the high transthoracic approaches were used（2 cases）. Excision of tumor was carried out according to demands of the Weinstein-Boriani-Biagini （WBB） staging system. Spine stability was reconstructed by bone autografting and instrumentation. There were 4 cases of primary tumor and 3 of metastases. Their mean age was 45. 1 years （23 to 66）. The mean follow-up was 18. 9 months （3 to 45）. Results were evaluated by occurrence of complications, improvement of symptoms, local recurrence and mortality. Results: All patients stood surgery well. No significant complications occurred during and after operation. Local pain was significantly alleviated and neurological deficit was improved at least one Frankel grade. Three patients died. Local tumor control was obtained in 6 patients （85. 7%） until the end of follow-up. Conclusion:Our experience showed that via low anterior cervical approach and high transthoracic approach, the cervicothoracic vertebrae tumor could be excised safely and adequately. Moreover, excision of tumor according to the WBB surgical staging system and reconstruction of spine stability have made great contribution to local tumor control and the neurological function improvement.0|9|0Updated：2026-03-12
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