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Volume ， Issue 3 ， 2006
- Abstract：<正>Objective: To investigate the expression of P2X receptors on rat intracardiac and paratradieal ganglion neurons. Methods: For preparation of intracardiac neurons, hearts were excised, the atria were separated and the medial region containing intracardiac ganglia was isolated and cut into pieces. For preparation of paratracheal neurons, the tracheas were removed and the superficial membranous layer containing paratracheal ganglia was rapidly isolated. Intracardiac and paratracheal ganglion neurons were dissociated after digestion by collagenase and trypsin. Whole-cell patch clamp recording was used to identify the pharmacological properties of P2X receptors in cultured neurons. Results:Neurons from these two ganglia responded to ATP with a rapidly activating, sustained inward current.αβ-meATP failed to evoke any responses in paratracheal ganglion neurons while a few of intracardiac ganglion neurons responded toαβ-meATP with a tiny sustained inward current. ADP and UTP had no effect on intracardiac neurons. Lowering pH potentiated ATP responses in neurons from these two ganglia whereas increasing pH inhibited ATP responses. Co-application of Zn2+ potentiated ATP responses in intracardiac and paratracheal ganglion neurons. Conclusion: The receptor subtypes involved in intracardiac and paratracheal ganglia appear to be homomeric P2X2, while heteromeric P2X2/3 could not be completely excluded from intracardiac neurons.0|34|2Updated：2026-03-12
- Abstract：<正>Objective: To analyze and identify differentially expressed phosphoproteins associated with mitochondrial KATP channel opening. Methods: Adult rat ventricular myocytes were isolated, cultured, and identified, and pretreated without or with 100μmol/L diazoxide for 10 min. Phosphoproteins prepared and enriched from the control and diazoxide-pretreated cells were separated by two-dimensional gel elec-trophoresis （2-DE） followed by sliver staining. The obtained interesting phosphoproteins were further i-dentified by mass spectrometry. Results: Associated with diazoxide preconditioning, the proteins of chap-eronin containing TCP-1 and hypothetical protein XP- 346548 were phosphorylated significantly （P< 0. 01）, while the 94-kDa glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphory-lated markedly （P<0. 01）. Conclusion: These findings suggest that cardiomyocytes undergo significant posttranslational modification via phosphorylation in a multitude of proteins in order to respond diazoxide preconditioning, and these phosphorylated protein may mediate the downstream signaling of cardioprotec-tion by mitochondrial KATP channel opening induced by ischemic preconditioning.0|10|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the effects of motilin and erythromycin on intracellular Ca2+ mobi-lization in cultured myenteric neurons of rats. Methods: The cultured myenteric neurons were identified with immunofluorescence staining technique. Motilin-induced and erythromycin-induced intracellular Ca2+ mobilization was studied in primary cultures of myenteric neurons using the ratiometric Ca2+ indicator Furo3/AM, with a laser confocal microscope. Results: The effects of motilin and erythromycin on intracellular Ca2+ mobilization were as follows: （1）In Hank’s solution, 10 -8, 10-7, 10-6 mol/L motilin could elevate intracellular Ca2+ concentration （[Ca2+]i）in a dose-dependent manner. （2） In Hank’s solution, 10μg/ ml erythromycin also could induce the elevation of [Ca2+]i. （3） After pretreatment with antibody against the motilin receptor in Hank’s solution, the Ca2+ response to erythromycin was almost restricted. Conclusion: It is suggested that motilin could increase [Ca2+]i in myenteric neurons in a dose-dependent manner, and erythromycin may also have this effectivenesss by binding to the motilin receptor.0|42|1Updated：2026-03-12
- Abstract：<正>Objective:To observe the effect of sodium citrate plasma （scP） on the growth and function of hepatocytes. Methods: HepG2, fetus and porcine hepatocytes were cultured. The viability, cell cycle and apoptosis, the leakage of LDH, AFP, total protein, glutathione and the changes on morphology of hepatocytes exposured to scP were investigated. Results: （1）Cultured in 10%, 30%, 50%, 100% scP for 24 h, the viability of HepG2 cells was inhibited （F = 40. 108, P = 0. 001）. After 48 h, nearly all cells died except 10% scP group. （2）Exposured to scP for 24 h,the percentage of S phase of the cell cycle was significantly increased and apoptosis was also significantly increased compared to control cultures. （3） The leakages of LDH were increased in the HepG2, fetus and porcine hepatocytes following exposure to scP for 5 h. （4） The synthesis of AFP in fetus and porcine hepatocytes were inhibited in medium containing 10% scP for 3 d（t values were 8. 1902, 5. 1034 separately, P<0. 01）. Exposure of HepG2 cells to scP within 24 h resulted in a decrease in the total protein synthesis and a increase in the GSH content. （5）Most of HepG2, fetus and porcine hepatocytes died in all except 10% scP groups after 24 h exposed to scP. Conclusion:scP can damage hepatocytes, which results from citric acid and sodium citrate contained in the fluid of blood maintenance.0|11|0Updated：2026-03-12
- Abstract：<正>Objective :To evaluate the possibility of the technology involving PEP and RDB for detectingβ-thalassaemia multipoint mutations from a single cell simultaneously. Methods: A set of allele specific oligonucleotide （ASO） probes used for detecting 8 familiarβ-thalassaemia mutations （CD41-42. IVS-Ⅱ-654, CD17, TATA box nt-28, CD71-72, TATA box nt-29, CD26, IVS-Ⅰ-5） were immobilized on a strip of nylon membrane. The genome of a individual cell was amplified by primer extension preamplification （PEP） with the mixture of 15-base random oligonucleotides. The aliquots from PEP were used to amplify the objective gene fractions ofβ-thalassaemia gene by nested or semi-nested PCR. The membrane was hybridized with the final amplified products and then treated with Streptavidin-HRP and color development. Results:Totally 30 lymphocytes were picked up from blood samples of 1 healthy female and 4 patients with knownβ-thalassaemia mutations respectively. Each single lymphocyte was lysed in the proteinase K buffer. The amplification efficacy was 94. 0% and alle drop-out （ADO） rate was 8. 0%. Revert dot blot （RDB） was applied to the final amplified products from the 5 participants. The results of diagnosis were the same to the expected, and their genotypes were N/N, CD17（A→T）/N, IVS-Ⅱ-654（C→T）/CD17（A→T）, CD41-42（-CTTT）/N and TATA box nt-28（A→G）/N, respectively. Conclusion: The technology involving PEP and RDB could detect multipleβ-thalassaemia mutations from a single cell simultaneously, and the research provides experimental evidences for the feasibility of applying PEP and DNA array technology to screening multiple genetic mutations from a single cell, and will be applied to preimplantation genetic diagnosis and non-invasive prenatal diagnosis forβ-thalassaemia.0|19|0Updated：2026-03-12
- Abstract：<正>Objective: To study the genetic variation of mitochondrial DNA（mtDNA） among common laboratory strains of inbred mice. Methods: The genetic polymorphism of mtDNA among 4 classical laboratory strains of inbred mice and 3 inbred strains of mice established in China was analyzed by polymerase chain reaction coupled with restriction fragment length polymorphism（PCR-RFLP） and PCR coupled with single-stranded conformation polymorphism（PCR-SSCP）. Results: With regard to the D-loop（Displacement loop,D-loop）,tRNAMet+Glu+（?）e,and ND3（NADH dehydrogenase subunit 3,ND3） gene fragments of mtDNA from these mice,no variation was revealed by PCR-RFLP at 46 restriction enzyme sites. Further analyzed by PCR-SSCP,the D-loop 5’fragment and 3’end fragment of mtDNA from these mice also showed no genetic variation. Conclusion: Owing to maternal mode of inheritance of mtDNA,the results indicate that these common inbred strains of mice share the same maternal lineage.0|14|0Updated：2026-03-12
- Abstract：<正>Objective:With the regular mixed lymphocytes culture （MLC） to detect the allograft rejection, the reactivity of the activated lymphocytes （primed lymphocytes） of a recipient shows sometimes increase and sometimes decrease against the antigens from the donor, which is inconsistent with the clinical results. In order to establish a convenient method for testing the specificity of the activated lymphocytes in vitro, so as to know the rejection occurred or not by testing the existence of the specific activated lymphocytes against donor’s HLA antigens in the recipient’s peripheral blood. Methods: Anti-IL-2 neutralizing monoclonal antibody （anti-IL-2 N-mAb） and immunosuppressors were introduced in this test system in the presence of specific stimulators and activated lymphocytes. Results: When the activated lymphocytes were chosen from the one-way MLC 4 d to undergo re-stimulation by specific stimulators, the activity of activated lymphocytes in the treatment group was suppressed significantly compared with that in the control group. The result of this test method is consistent with the biopsy in the clinical diagnosis of rejection. Conclusion:It suggests that the activated lymphocytes can be inactivated by specific antigens in certain conditions. This can be a useful tool to define the specificity of the activated lymphocytes.0|7|0Updated：2026-03-12
- Abstract：<正>Objective: To look for additional markers of molecular biology response to anastrozole, a new aromatase inhibitor, on the growth and mRNA expression level of MCF-7 cell. Methods: We investigated the effect of anastrzole on growth and gene expression in the human breast cancer cell line MCF-7 and compared with the most widely used antiestrogen tamoxifen. We chose 4 genes to examine regulation of gene expression of estrogen regulated genes: PR A, PR B, ErbB-2 and cyclin D1. Results: Compared with the tamoxifen, a statistically significant growth inhibition was observed with anastrozole. The PR A, PR B and cyclin D1 mRNA level in anastrozole treated cells was sigificantly below the level in tamoxifen treated cells （P<0. 05）. They had agonistic effect on ErbB gene （P>0. 05）. Conclusion: The third generation of aromatase inhibitors anastrozole exert more inhibit function in some expression of estrogen regulated genes than tomoxifen in MCF-7 cell line.0|41|0Updated：2026-03-12
- Abstract：<正>Objective: To observe osteogenetic rate of alveolar bone on the tension side in orthodontic tooth movement through distraction osteogenesis of the periodental ligament quantificationally. Methods: The experiment was carried in 6 dogs. The left side of jaws of each one was set as test or control side, and the other side was control or test side. On the control side, the first premorlar was moved by traditional method on the test side. A self-made distraction device was used on the test side. The newly formed alveolar bone on the tension side of moved tooth was labeled by serial tetracycline fluorochrome. Sections were observed by fluorescence microscope and pictured. Newly formed bone was measured by computer image analysis. Results: The quantity of newly formed bone was significantly different between the two methods. Newly formed bone in rapid tooth movement by distraction osteogenesis of the periodental ligament was more than that in traditional method. Conclusion: The distraction through periodental ligament could induce more rapid bone formation and excite higher osteogenetic activity than traditional method.0|36|0Updated：2026-03-12
- Abstract：<正>Objective:To expound geographical information system （GIS） technology is a very important tool when it was employed to assist to present the distribution by time and place and the model of transmission of infectious disease. Methods: We illustrated the assistant decision-making support function of GIS with an example of the spatial decision support system for SARS controlling in Shaanxi province of China which was developed by us. Results: The spatial decision support system established by applying GIS technology fulfilled the needs of real-time collection and management and dissemination SARS information and of surveillance and analysis the epidemic situation of SARS. Conclusion: Occurrence and epidemic of diseases, implement prevention and intervention measures and collocation hygienic resources are all with the characteristic of the variation of time and space, therefore, GIS technology has become a powerful tool for identifying risk factors of diseases, providing clues of causation of diseases , evaluating the effects of intervention measures and drawing a health management plan.0|33|3Updated：2026-03-12
- Abstract：<正>Objective:To evaluate the imageologic characteristics of the bronchial arteries （BAs） in primary lung cancer （PLC） with multidetector CT （MDCT） angiography. Methods: Thin-section enhanced CT scanning （with an Toshiba Aquilion 16 scanner） was performed in 164 PLC patients, of whom 123 were confirmed by pathology and the remaining 41 were confirmed by typical radiological and clinical findings. Another 46 patients with normal thoracic CT presentations were served as control. Three-dimensional （3D） images of the BAs were processed at workstation （Vitrea 2, Vital Corp, USA）. Spatial anatomical characters of the BAs were observed using volume rendering （VR） and muhiplanar reconstruction （MPR） or maximum intensity projection （MIP）. Results: At least one bronchial artery was displayed clearly on VR in 152 （92. 7%） of the 164 PLC patients and 32 （69. 6%） of the 46 controls. There were 48. 92% of the right BAs originating from the descending aorta and 46. 24% from the right intercostal artery. 97. 53% of the left BAs originated from the descending aorta, and 94. 87% of the common trunk from the descending aorta. There were 10 distribution patterns of the BAs, with one on the right and one on the left predominating （48. 68%）. More BA branches were found to reach far from the segmental bronchi or enter into the lesions in the PLC group than those in the control group （25. 8% vs 1. 7% ）. and also the ipsilateral side of the PLC than the contralateral side （40% vs 8. 8%）. The diameter and the total transaxial areas of the BAs on the ipsilateral side of the PLC lesions were significantly larger than those on the contralateral side or those of the control group （P<0. 05）. Conclusion:The anatomic characters and pathologic changes can be depicted in vivo stereographically and clearly by CTA with volumetric 3D rendering. Dilation of the BAs and increase of total blood flow in patients with PLC can be evaluated quantitatively, which may be useful in the diagnosis and assessment of PLC, and have the potential to increase the-safety and effect of interventional therapy.0|12|3Updated：2026-03-12
- Abstract：<正>Objective:To investigate the outcomes of subcutaneous island pedicle flap for reconstruction of the medium-sized facial skin defects. Methods: Eighty nine cases of facial defects within one cosmetic unit following removal of skin tumors or scars were reconstructed with advancement or transposition island pedicle flaps. Patient records and postoperative photographs were reviewed retrospectively. Both patients and other surgeons were asked to assess outcome variables. Results: All flaps survived with primary healing postoperatively. Patients and surgeons judged excellent or good overall outcomes in 95. 5% and 92. 7% of all evaluated cases, respectively. Conclusion: The subcutaneous island pedicle flap may be an extremely versatile and reliable method for aesthetic reconstruction of medium-sized facial defects in the patients of all time of life.0|9|0Updated：2026-03-12
- Abstract：<正>Dense and extensive esophageal strictures after caustic agent ingestion require surgical treatment. Colon, stomach and jejunum can be used to reconstruct esophagus. Here, we report an unusual patient with corrosive esophageal stricture who had received unsuccessful esophageal replacements twice at other hospitals. Colon interposition had been first performed 6 months after corrosive esophageal burn, but the colon graft necrosis occurred. Esophageal reconstruction had been carried out 10 years later in another hospital. However, the graft necrosis developed again 5 months later. A salvage operation was performed to remove the necrotic transplant in our hospital. Then as much food as possible had been given to expand the stomach through the gastrostomy since the procedure. The patient underwent esophagecto-my and concomitant gastroesophagostomy in the neck 1. 5 years later. Esophageal dilations had been performed to prevent recurrent anastomotic stricture for 1 year. He has eaten a normal diet since being discharged.0|6|0Updated：2026-03-12
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