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Volume ， Issue 3 ， 1992
- Abstract：<正> The changes of blood-brain barrier（BBB）permeability following brain injury werestudied quantitatively by using colloidal gold（CG）particles of various sizes as tracers in 57 rab-bits.In addition,water content in brain tissues was determined.The brain-injured rabbits re-ceived intravenous injection of anisodamine in a dose of 0.3 mg/kg BW at 5rain or 3h after braininjury,and the effect of anisodamine on aherations of BBB permeability and water content of thebrain tissue after brain injury was investigated.The results indicated that an increase of BBBpermeability began at 30min after injury with only a few of 5nm CG tracers present in the endo-cytic pits and endothelial microvilli,and there were 10nm CG tracers passing through the BBB at3 h after brain injury.The increase of BBB permeability reached its peak at 6 h after brain in-jury.More 15nm CG tracers penetrated the BBB by way of pinocytotic vesicule transportationand opening of tight junctions between endothelial cells.The water content in the brain tissueincreased,which was closely correlated to the above-mentioned EBB permeability and giving ad-vantages to the treatment of traumatic brain edema in the present study.0|19|1Updated：2026-03-12
- Abstract：<正> The changes of the cytoplasmic free calcium level in the neutrophils after smoke in-halation injury were observed in rabbits and then the effects of tetrandrine,a calcium antago-nist,on the changes of free calcium level were studied.It was found that the number of neu-trophils increased significantly preceded by a transient decrease in the blood and also increasedin the bronehoalveolar lavage fluid after smoke inhalation.and the level of cytoplasmic free calci-um in the blood neutrophil increased likewise.Administration of tetrandrine resulted in a reduc-tion of the neutrophils number in the lungs and the free calcium level in the blood neutrophils toalleviate the pulmonary injury due to smoke inhalation.It is believed that there is a close rela-tionship between the activation of neutrophils and the pathophysiological changes of the lungs,and tetrandrine can exert its therapeutic effects on the injury by decreasing the free calcium levelin the neutrophils to modulate their functions.0|6|0Updated：2026-03-12
- Abstract：<正> The high-affinity glucocorticoid binding sites（HAGS）and the low-affinity glucocor-ticoid binding sites（LAGS）with steroid specificity were demonstrated in cerebral cytosol ofrats by using the radioligand binding assay.The equilibrium dissocation constant（Kd）of HAGSand LAGS were（2.78+0.71）×10-8mol/L and（2.12±1.06）×10-6mol/L respectively as esti-mated by Scatchard and Pseudoseatchard analysis.Glucocorticoid receptors（GR）in the trau-matized（left）hemisphere cytosol were decreased more significantly than those in both the con-trol（right）hemisphere cytosol at 6 h postinjury and normal brain tissue（P<0.05）,but Kd ofGR showed no significant changes.GR of liver cytosol at 6h postinjury were more markedly de-creased than normal hepatic cytosol,but Kd of GR underwent no significant changes.These da-ta demonstrate that high-dose glucocorticoid（GC）used in the treatment of traumatic brain ede-ma might maintain target-cell reactions by increasing the production of GC receptor complexesand is most likely to be mediated by LAGS.0|3|0Updated：2026-03-12
- Abstract：<正> One oligonueleotide probe,HIV env 101-1,and two oligonucleotide primers,HIV env125-1 and HIV env 125-2,were designed with the aid of a computer and synthesized by a DNAsynthesizer.The env 125 peptide gene was amplified by polymerase chain reaetion（PCR）.Beingidentified by electrophoresis and Southern blotting,the PCR product was cloned into plasmidpUC 19.The recombinant pENV 125,identified with X-gal selection and restriction mapping, wassequenced.The results showed that the cloned env 125 peptide gene contained the inserted EcoRⅠ site and ATG at the 5’end,Hind Ⅲ site and TAG at the 3’end.The sequence and readingframe were proved to be correct.0|7|0Updated：2026-03-12
- Abstract：<正> The sensitivity of PCR was determined for detection of HBV DNA in paraffin-embed-ded liver tissue with different methods for sample preparation.Of 8 cases of HBeAg-positiveHBV infection,HBV DNA was detected in 6 by extracting DNA from both frozen and dewaxedsamples,but in none by direct reaction.Of 12 cases subjected to Southern blot hybridization,HBV DNA was detected in 7 by this technique,in 10 by PCR with both methods of DNA extrac-tion and in 3 by direct PCR.The results showed that PCR was sensitive and was comparablewith blot hybridization in detecting intrahepatic HBV DNA.In comparison between differentmethods of sample preparation,the viral detection rate from the dewaxed samples was near thatfrom the frozen ones,while by the direct reaction HBV DNA could be detected only in a fewsamples with high level of infection.0|8|0Updated：2026-03-12
- Abstract：<正> Reviewing the clinical data of 57 burn cases,the authors suggested that the postburnrespiratory failure could be classified,according to the time it occurred,into 3 types:（1）Earlytype,occurring in the shock stage with severe inhalation injury being its main cause.（2）Delayedtype,developing in the postburn acute infection stage.Its clinical course was similar to that ofadult respiratory distress syndrome,and severe shock and systemic infection were the maincauses.（3）Late type,occurring in the stages of wound healing and rehabilitation.Its etiologi.calfactors included severe hypoproteinemia,malnutrition,infection and pulmonary embolism due tothrombosis of the inferior vena cava.The new classification was proposed because it was be-lieved to be helpful in the prevention and treatment of respiratory failure in different stagespostburn.0|13|0Updated：2026-03-12
- Abstract：<正> The effects of hydrogen peroxide（H2O2）on endothelial-polymorphonuclear leuko-cyte（EC-PMN）adhesion and their mechanisms were studied in cultured bovine pulmonaryartery endothelial monolayers in vitro.H2O2 at various concentrations(10-3,10-2,10-1mol/Lrespectively)stimulated EC-dependent PMN adhesion,of which 10-2mol/L H2O2 was the mostpotent one,increasing adhesion to 2.3 times that of the control.Pretreatment of PMNs with SRI63-441,a platelet-activating factor（PAF）receptor antagonist,had no inhibition effect on H2O2induced EC-PMN adhesion.Pretreatment of ECs with SRI 63-441 before H2O2 exposure signifi-cantly decreased PMN adherence to ECs.Pretreatment of ECs with phospholipase A2 inhibitorp-bromophenacyl-bromide or calmodulin antagonist chlorpromazine and calcium ion chelate EG-TA obviously decreased H2O2 induced increment of EC-PMN adhesion.These results suggestthat H2O2 may activate ECs,causing the inflow of extracellular calcium or the release of calciumfrom intracellular deposits.Increased intracellar Ca2+may bind with calmodulin to activate phos-pholipase A2,thus initiating PAF synthesis and promoting EC-PMN adhesion.0|3|0Updated：2026-03-12
- Abstract：<正> Absorptive process of 3H-5-hydroxytryptamine（3H-5-HT）in the intestinal lumenwas studied by radiochemical method and autoradiography.The results indicated that 3H-5-HTinjected into the intestinal lumen was rapidly absorbed.The absorptive peak appeared at 1 h af-ter 3H-5-HT injection.Part of the absorbed 3H-5-HT might enter the blood circulation,andthe other part was located specifically in the submucous plexus,myenteric plexus and some ep-ithelial cells.Whether these neurones and epithelial cells are 5-HT containing cells or are cellswith specific binding sites awaits further studies.0|3|0Updated：2026-03-12
- Abstract：<正> It has been developed in this laboratory that a serum-free medium, designated asDMI,is adaptive for long-term culture,freezing and resurgence in liquid nitrogen,and cloningof hybridoma and parental myelomas as well as for cell fusions.In this report,it is describedthat the myeloma cells grown in DMI for more than 3 months maintained their biological charac-teristics such as induction of aseites and subcutaneous somatic tumor in BALB/c mice and toler-ance toward 8-azaguanine,ete..However,the ability of the tumor cells to attach to glass wallwas decreased.The selecting assay of these cells in HAT medium（medium containing hypoxan-thine,aminopterin and thymidine）showed that the death time in DMI was similar to that in theconventional 15％ newborn calf serum-supplemented medium（RPS15）.The hybridoma cellsadapted in DMI secrete monoclonal antibodies in quantities comparable to those produced inRPS15.0|10|0Updated：2026-03-12
- Abstract：<正> Left ventricular contractility was measured using Doppler echocardiography in 37 pa-tients with rheumatic mitral valve disease.Myocardial pathological lesions were studied quanti-tatively by means of the image analysis system and light microscopic examination.The resultsshowed that attenuated cardiac function is due to pathological change of the mitral valve andpathological lesions of the left ventrieular myocardium including increased interstitial fibrosis,thickened intramyocardial arterial wall leading to concentric narrowing of the lumen,hyper-trophic myocardial fiber and endocardial proliferation.Since mitral valve replacement can notcorrect the irreversible myocardial pathological lesions,perioperative protection of the my-ocardium should be emphasized,and intraoperative injury to the myocardium should be mini-mized as far as possible.0|8|0Updated：2026-03-12
- Abstract：<正> Neuroleukin（NLK）,a protein（Mr 56000）,acts on both the nervous and the im-mune systems and its expression is regulated at mRNA levels.One hundred fifty Sprague-Daw-ley（SD）male rats were divided into 5 groups:control group（N）and groups of cold-exposurefor 1,2,3 and 4 weeks（ClW,C2W,C3W and C4W）.With RNA dot blot experiments,changes of NLK mRNA levels in both neuroendocrine system（pituitary and hypothalamus）andimmune system（splenic lymphocytes,LC）of SD male rats during cold acclimation presentedsimilar responses.C/N ratios of scan gray intensity level in RNA dots are presented as follows:there was a rise in C/N ratio to 1.63～11.31 in CIW,a fall to 0.63 in C2W and a rise again to1.42～21.09 in C3W.It might thus be regarded as a general response to cold surroundings.Itwas reported that SDS-PAGE autoradiogram of synthetic proteins in LC and secretive proteinsin supernatant cultured in vitro showed that NLK content in LC was not parallel to its mRNAlevel because NLK was extremely meager over the 1-week cold exposure whereas it increasedobviously over the 3-and 4-week cold exposure.This is probably a major cause of why im-munoglobulin synthesis in cold-acclimated animals was greatly enlarged.0|5|0Updated：2026-03-12
- Abstract：<正> In order to investigate the cardiovascular effects of the scorpion（Buthus martensiKarsch）venom（BmKv）,the left ventricle of the rats was catheterized via the right carotidartery.The LVP,LVEDP,+dp/dt max,Vmax,HR and BP were observed.The results showedthat intravenous injection of the BmKv（60μg/kg）,in comparison with the control,elicited obvi-ous hypertension and increase of cardiac contractility,both of which lasted for 1h,while theheart rate had no significant change rand that pretreating the rats with alpha-adrenergic blocker,phentolamine,antagonized the hypertensive effects,but did not antagonize the increase of cardiaccontractility.Pretreatment with beta-adrenergic blocker,propranolol,has no influence on the ef-fects of the venom.It is suggested that the hypertensive effects are due to the activation of al-pha-adrenergic receptor,whereas the increase of cardiac contractility may not be resulted fromthe activation of beta-adrenergic receptor.The BmKv treated with dithiothreitol before injectionhad no cardiovascular effects,indicating that the intact disulfide bridges play a decisive role inthe cardiovascular effects of the BmKv.0|4|0Updated：2026-03-12
- Abstract：<正> Arterial blood gases and electrolytes were determined in 159 cases of adult respirato-ry distress syndrome（ARDS）.It was found that disordered acid-base balance was a commonfinding in various kinds of ARDS.In mild ARDS,respiratory alkalosis and the combination ofrespiratory alkalosis plus metabolic alkalosis or metabolic acidosis were usually encounted,whilein moderate and severe cases of ARDS,triple acid-base disorders,respiratory acidosis,and thecombination of respiratory acidosis plus metabolic acidosis were commonly seen.Severe alkalosiswas one of the factors to result in death.0|3|0Updated：2026-03-12
- Abstract：<正> The number of AgNORs in the nucleolar organizer region was studied quantitativelyby one-step silver colloid technique in 6 samples obtained from normal bladder tissue,9 ofchronic cystitis and 56 of transitional cell carcinoma of bladder.The results revealed that meannumber of AgNORs per nucleus was not statistically different between normal bladder tissueand tissue from chronic cystitis,while there was a significant difference between normal or in-flammatory tissue and malignant tisssue on the one hand and between various grades of bladdertumor on the other.The results also indicated that mean number of AgNORs per nucleus wasclosely related to the prognosis of bladder tumor.So it is suggested that AgNOR counting bythe silver stain technique might play a role in the differenciation of benign and malignant lesionsand in grading and prognosis in bladder tumors.0|7|0Updated：2026-03-12
- Abstract：<正> Sections of 30 cases of human cirrhosis were stained with rabbit anti-insulin-likegrowth factor Ⅱ（IGF Ⅱ）by double PAP method.By the serological examination 15 patientsshowed HBV infection and sections of 14 eases were HBsAg postively with a total rate of 67％（20 cases）.The IGF Ⅱ was positive in the cytoplasm of all the liver and ductular cells.Binucle-ated,polypoid liver cells and the peripheral cells of the lobules or nodules were distinctly posi-tive,The liver cells which were strongly positive were a kind of thin polygonal cells with asmall oval or a round deeply stained nucleus in each.They might exist sporadically in the lob-ules or in the marginal portion of a nodule.These liver cells are quite different from the so-called oval cells which are derived from the proliferating ductules and are generally believed tobe responsible for the pathogensis of hepatoma.0|7|0Updated：2026-03-12
- Abstract：<正> Acid phosphatase（ACPase）-positive nematolysosomes in mouse hepatocytes and thepancreatic exocrine acinar cells were studied with ultracytoehemical method.The nematolyso-somes were 2～8μm in length,0.1～0.3μm in diameter.Some branched and some did notbranch,and most of them winded through the organelles.0|5|0Updated：2026-03-12
- Abstract：<正> The effects of tumor necrosis factor（TNF）on the cultured mouse hepa-tocytes and non-parenchymal liver cells were observed.It was found that therewere no significant changes of the morphological integrity and viability of thehepatocytes and the aspartate transferase level in the culture supernate after theaddition of TNF into the culture medium as compared with those of the normalcontrol,which indicates that TNF exerts no obvious cytotoxocity on the culturedmouse hepatocytes. In addition,there were also no significant changes of theabove mentioned parameters after TNF was added to the cocultures of hepato-cytes and non-parenchymal liver cells,which implies that the unactivated non-parenchymal liver cells are not involved in the TNF-related hepatocyte injury.0|4|0Updated：2026-03-12
- Abstract：<正> From July 1988 to May 1991,the authors had used the microwave tissue thermo-co-agulation method（MTTC）to treat 76 cases of large cavernous hemangiomas in the oro-maxillo-facial region.When a microwave antenna-pin was inserted into the tumor,blood and tissuewithin the tumor began coagulating due to high temperature produced by the mierowave ma-chine.Along with a full coagulation,the tumor may be removed surgically without bleeding.This method has many advantages:（1）less bleeding with little need of blood transfusion;（2）convenience for excision;（3）time for operation markedly shortened;（4）suitability for opera-tion-contraindicated patients.The authors consider that the microwave tissue thermo-coagula-tion method is a rather ideal method for treating large cavernous hemangiomas in the oro-maxil-lo-facial region.In this paper are reported 76 eases of clinical application and animal experimentof microwave tissue thermo-coagulation influences on the muscles and vessels.0|5|0Updated：2026-03-12
- Abstract：<正> ECGs of 3 565 clinically normal male pilots,aged from 21 to 51 years,were classi-fied and analyzed by Minnesota code.Among the 3 565 pilots,there were 1 315 coded pilots,making up 36.89％ of the total number.The results were as follows:abnormal Q/QS wave0.14％,electrocardial axis deflection 5.11％,high voltage of left ventricle 10.58％,chargedST-T segment 2.07％,atrioventricular conduction block 1.71％,intraventricular conductionblock 4.60％,arrhytmias 10.66％ and other abnormalities 13.04％.The research showed thatthere were various changes in the coded pilots,although no abnormal alteration of the cardio-vascular system was discovered by physical examination,the ECG exercise stress test and otherrelevant clinical tests.This suggests that most of ECG abnormalities in clinically normal pilotsare normal physiological and functional variations.0|2|0Updated：2026-03-12
- Abstract：<正> The monoclonat antibodies（McAbs）against Japanese B encephalitis virus for clinicaluse were prepared according to the standards of Food and Drug Administration（FDA,USA）.Immediately after the diagnosis,the patients were given 10 mg of McAbs（IgG）in normal salineby intravenous drip plus intramuscular injection.The average febrifugal days of the McAb treat-ment group（55 cases）,symptomatic treatment group（12 cases）,ribavirin treatment group（17cases）and thymus peptides treatment group（7 cases）were 2.86,6.20,7.16 and 9.11d,respec-tively（P<0.05 or P<0.01）.It is necessary to take the skin test before use of the McAbs andto give dexamethasone during the McAb treatment.0|4|1Updated：2026-03-12
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