Latest Issue

    Volume Issue 2 1990
    • 吴皓, 李兆基, 萧轼之, 周水淼, 罗申
      Issue 2, Pages: 95-100(1990)
      Abstract:<正> The purpose of this work was to reestablish the respiratory abduction of theparalyzed vocal cord through reinnervation of the posterior cricoarytenoid(PCA)mus-cle by part of phrenic fibres.In fifteen adult cats the adductor branch of the recurrentlaryngeal nerve(RLN)of the right side was cut and its distal end ligated,while the pro -ximal end was implanted into the PCA muscle belly.The whole RLN was then transectedin the tracheoesophageal groove and its distal stump anastomosed to the upper branchof the phrenic nerve.Various techniques for observation were used on day 40,80 and 150after operation.Direct laryngoscopy showed that the inspiratory abduction of the para-lyzed vocal cord recovered within 40 d in all cats.Eighty days later,a larger abducentmotion of the glottis was observed on the reinnervated side.Abduction was caused byreinnervation of the PCA muscle from phrenic motoneurons,as demonstrated by laryn-geal electromyogram,and the function of diaphragm maintained as revealed by monito-ring of the intrathoracic pressure.  
        
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    • Preparation of Chronic Prefemoral Lymphatic Fistula in Goats.

      Jin Lijuan
      Issue 2, Pages: 100(1990)
      Abstract:<正> We have detailed the preparation of chronicprefemoral lymphatic fistula in goats.The prefe-moral lymph node receives afferent vessels fromthe skin of the posterior thorax,abdomen,pelvis,and thigh and from the tensor fascia lata.To col-  
        
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    • Experimental Observation on Treatment of Malignant Tumor with Direct Current.

      Huang Zhuoyuan
      Issue 2, Pages: 100(1990)
      Abstract:<正> In order to investigate the possibility of trea-ting malignant tumors with direct current,thechanges of murine S1(?)0 type sarcoma was obser-ved under different electrodes and through differ-ent duration of treatment.The results showedthat in tumor tissue liquefacient and coagulativenecrosis occurred around either negative or posi-  
        
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    • Determination of the Halflife of Red Blood Cells in Vitro.

      Peng Wuhe
      Issue 2, Pages: 105(1990)
      Abstract:<正> This paper reports a new technique determin-ing the half-life of the RBC. The traditional me-thod was replaced by the in vitro 51Cr-RBC me-thod which needn’t take out blood from theanaemic patients frequently,and needn’t infuseradioisotope into the body of patient.In our ex-periments,the correlation was very significant be-  
        
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    • Preparation of Rabbit Antihuman Albumin Serum and Purification of the IgG Antibodies.

      Lan Fenghua
      Issue 2, Pages: 105(1990)
      Abstract:<正> We applied a very simple method,TCA-al-cobol method,to purify human serum albumin.The product is pure albumin by SDS-PAGE.Emulsified with Freund’s complete adjuvant(FCA),it was used to immunize rabbits by a pro-cedure which lasts only five weeks.Specific anti-serum with high titration(1:128)was obtained.  
        
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    • Abnormalities of Cellular Immunoregulation in Patients with Epidemic Hemorrhagic Fever

      陈龙邦, 杨为松, 徐海峰, 张文彬, 朱平, 尚高峰
      Issue 2, Pages: 106-112(1990)
      Abstract:<正> In this report,a comparative study is made of the function test of spontaneousT suppressor cell(STs)and T Lymphocyte subsets in patients with epidemic hemorrha-gic fever(EHF).It was found that in the early stages of the disease the activity of STs wasmarkedly lower than that of the controls,while the percentage of CD?+ cells increasedsignificantly,which led to the decrease and reciprocation of CD4/CD8 ratio,and that theactivity of STs was reversely related to the proportion of CD8+ cells on linear regressionanalysis,indicating that the CD8+ cells increased may mainly belong to cytotoxic T cells.It was also shown that the changes of STs function and CD4/CD8 ratio were related tothe abnormalities of serum C3 content and circulating immune complex.The results sug-gest that the disturbance of host cellular immunoregulation may play an important rolein the pathogenesis of EHF.  
        
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      Updated:2026-03-12
    • A Study on Competitive Nylon Solid Phase Radioimmunoassay for Determination of α-fetal Protein

      苏殿杰, 王世中
      Issue 2, Pages: 113-116(1990)
      Abstract:<正> A method of nylon solid phase radioimmunoassay(SPR)was developed andused in the determination of α-fetal protein(AFP).Nylon grains were activated and con-jugated with IgG to form a solid phase IgG.The activity of solid phase IgG was stableand it can be maintained for near 3 years at 4℃.At room temperature the activity didnot obviously change in 13.7 months.The method is simpler and more convenient thanliquid phase radioimmunoassay(LPR).The reactive error relation(RER)of this methodwas 0.01 and the precision curve was perfect.Sixty seven samples were measured withboth the solid phase method and double antibody-PEG(polyethylene glycol).Results ofthe two methods were not significant in difference(P>0.05).The correlation coefficientof the two methods was 0.97(P<0.01).It was proved that nylon was a very good solidphase carrier for SPR.  
        
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    • Observation of Surface Ultrastructure of Pulmonary Large Carcinoma Cell in Pleural Fluid

      Ji Chenyang Zhang Yixun
      Issue 2, Pages: 122(1990)
      Abstract:<正> There are relatively specific morphologic fea-tures in the pulmonary large carcinoma cells un-der scanning electron microscopy(SEM).SEMreveals many kinds of surface ultrastructures onthe cellular surface and presence of giant flatten-  
        
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    • Changes of Intestinal Caicitonin Gene-related Peptide in Acute Intestinal Radiation Sickness in Rats

      吴康, 蔡文琴
      Issue 2, Pages: 123-127(1990)
      Abstract:<正> The characteristic distribution of calcitonin gone-related peptide(CGRP)inthe small intestine of rats and its changes in acute intestinal radiation sickness(AIRS)were studied with immunocytochemistry(whole mount stretch preparations of the smallintestine and cryostat sections)and radio-immunoassay.It was found that in all the lay-ers of the intestinal walls,there were large amounts of CGRP immunoreactive(CGRP-I)nerve fibers which existed in especiaUy high density in the myenteric,submucosal andmucosal plexuses.There was also a rather high density of the nerves around the smallvessels of the small intestine and the intestinal crypts.Some CGRP-I neurons were seenin the myenteric and submucosal plexuses.In AIRS,the intestinal CGRP showed a dip-hasic change,in a lower level in the 24th h and a higher level in the 48th and 72nd h af-ter irradiation.The results indicate that CGRP may be related to the regulation of the motility,se-cretion,absorption,sensation,and regional blood flow of the gastrointestinal tract.Pro-bably,CGRP is released under the stress of AIRS and participates in the mechanism ofinjury through many ways especially through the influence on the regional blood flowand the increase of the permeability of blood vessels.  
        
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      Updated:2026-03-12
    • Experimental Study and Clinical Observation on Therapy of Chronic Gastritis with Shen Xiang Yang Wei San.

      Sun Guihua
      Issue 2, Pages: 127(1990)
      Abstract:<正> According to the basic theory of the tradi-tional Chinese medicine,we have composed aprescription called Shen Xiang Yang Wei San(SXYWS,a powder)to treat chronic gastritis.After intravenous injection,it can evidently incr-  
        
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    • Analysis of Time and Frequency Domain of Auditory Brainstem Response in Normal Guinea Pigs

      郭梦和, 王锦玲, 吴进根
      Issue 2, Pages: 146-150(1990)
      Abstract:<正> The auditory brainstem response (ABR) was measured for 90 cars of norml gui-nea pigs,from which the mean latency,amplitude and recognition of waves were obtain-ded.The waves were classified into six types,with wave Ⅲ as the main wave which ac-counted for 77.1% of all the waves.The frequency domain of each type was analysed bymeans of digital filtering and auto-regressive (AR)estimation.The main frequency com-position of ABR in the guinea pigs was restricted within 1600 Hz,in which there werethree peaks at AR spectrum,the mean value of the peaks being 107.33 Hz,566.67 Hzand 1076 Hz respectively.The AR spectrograms of all types waves were very similar toeach other.  
        
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    • Yang Yili
      Issue 2, Pages: 150(1990)
      Abstract:<正> There was no significant increase of serumglucocorticoid in partial adrenalectomized ratsafter scalding,whereas glucocorucoid receptor inhepatic cytosol of those rats tended to increase.And 6h after scalding,the difference betweenscalded and controlled rats was highly significant.  
        
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    • 娄之聪, 陈水村
      Issue 2, Pages: 151-156(1990)
      Abstract:<正> Like electroacupuncture (EA),electrical stimulation of the dorsal raphe nucle-us (DRN)also inhibits the cAMP induced cortical epileptic waves and the EA inducedinhibition can be abolished or markedly reduced by iv cyproheptadine (CYP) or destruc-tion of DRN by electrolysis.The results suggest that 5-HT neurons in the DRN mayplay an important role in the EA inhibition of the generation of cAMP induced elec-trocorticogram (ECoG) epileptic waves.  
        
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    • Effects of Soman on Lymphocyte Proliferation in Mice

      董兆君, 王廷治
      Issue 2, Pages: 161-164(1990)
      Abstract:<正> Experiments to investisate the effects of soman on lymphocyte proliferation werecarried out on balb/c mice,which were injected subcutaneously with soman in the doseof 154μg/kg (0.8 LD50),96μg/kg (0.5 LD50) and 38.4μg/kg (0.2 LD50) respectively.Itwas found that from the 1st to the 7th day after poisoning with 154μg/kg soman,B-lymphocyte proliferation was severely inhibited (P<0.05),and it returned to the controllevel on the 10th day.Con A-stimulated T-cell proliferation showed a diphasic change,increasing at first and then markedly decreasing thereafter,after soman poisoning.The re-sults imply that the mitogenic response of both B- and T- cells and the primary increaseof T- cell response are closely related to the dosage of soman but not with the changes ofwhole blood cholinesterase activity.  
        
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    • Quantitative Analysis of DNA Content of Adenocarcinoma Cells in Effusion

      季晨阳, 张义勋
      Issue 2, Pages: 171-175(1990)
      Abstract:<正> DNA content of cells in effusion of 33 cases of mesothelial and adenocarcinomacells was measured by microspectrophotometer.The results showed that compared withmesothelial cells the DNA content of the suspected malignant cells was obviously increa-sed (P<0.01),the DNA content of both highly and poorly differentiate adenocarcinomacells was obviously increased and statistically significant difference existed between them(P<0.01);besides,aneuploidy could be found.It is objective,accurate and sensitive todetermine the character and differentiation degrees of adenocarcinoma cells in effusion byquantitaive analysis of DNA content with microspectrophotometer.  
        
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    • The Effects of Ginsenosides on Smoke Inhalation Injury in Rats

      张敏, 黄钺华, 李新芳
      Issue 2, Pages: 176-180(1990)
      Abstract:<正> A rat model was used to explore the therapeutic effects of ginsenosides (GS)on smoke inhalation long injury.It was found that GS could markedly alleviate the in-crease of pulmonary microvascular permeability (PMVP),reduction of protein and leu-cocyte content in the bronchoalveolar lavage fluid (BALF) of the smoke inhalation injur-ed rats.Histopathological studies of the lungs revealed that GS could distinctly reduceleucocyte accumulation in the vessels,interstitial infiltration of leucocytes,interstitial andintra-alveolar edema,hemorrhage and vascular congestion.Meanwhile,GS could inhibitthe elevation of malondialdehyde (MDA) in the lungs and serum and reverse the decrea-sed activity of superoxide dismutase (SOD) in the lungs after smoke inhalation.In addi-tion experiments in vitro also showed the inhibition of lipid peroxidation in lung homo-genate and elimination of superoxide anions hydroxyl radicals effectively by GS in properdoses.These results imply that there is close interrelationship between the therapeuticefficiency of GS on smoke inhalation lung injury and its capability of antioxidation.  
        
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    • Changes of Gastric Mucosal Blood Flow in the Healing Process of Peptic Ulcers

      李兆申, 周岱云, 许国铭, 陈菊初, 施雅芳, 姚光弼
      Issue 2, Pages: 181-186(1990)
      Abstract:<正> Changes of gastro-duodenal mucosal blood flow (GDMBF) in the healingprocess of 82 patients with gastric ulcer,130 with duodenal ulcer and 9 with active ul-cer induced by gastric polypectomy were studied by endoscopic laser Doppler flowme-try.In 108 normal subjects,the mucosal blood flow at the angle and antrum of the les-ser curvature and on the anterior wall of the duodenal bulb was the smallest (P<0.01).In patients with gastric ulcer,gastric mucosal blood flow (GMBF)significantly decrea-Sed at the active stage and increased at the healing stage (P<0.01 ).GDMBF significan-tly increased (P<0.01) at the active stage and returned to normal at the healing stage ofduodenal ulcer.GDMBF was unchanged in the healing process of the active ulcer inducedby gastric polypectomy.The mucosal blood flow at ulcer centre and margins significan-tly decreased at the active stage and markedly increased at the healing stage as compa-red with that of surrounding mucosa.We conclude that GMBF plays an importantrole in the developing and healing process of peptic ulcers.  
        
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