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Volume 25 ， Issue 3 ， 2010
- Abstract：Epidemiology studies infectious diseases as primary objects,and has formed a scientific system.With the continuous expansion of the scope in epidemiological studies,the development of research methods and the mutual penetration of related disciplines,epidemiology gradually differentiates into many branches.Military epidemiology is just a branch which serves military health and prevention works with epidemic theory.Its study objects are the officers and soldiers in the armed forces,and its mission is to focus not only on peace time,but also on war time and fighting time to prevent from infectious diseases,or even to smash the enemy’s biological war,which is basically different from the general epidemiology.In recent years,with the policy of reform and opening up,and owing to the improvement of people’s living standard,the military has significantly improved its living conditions.Meanwhile,because strategic policy and military training has been updated technical difficulty and intensity in the army have been increased,and disease spectrum has greatly changed.And especially as the theories and methods of modern epidemiology have been introduced into the army,the military epidemiology has been rapidly developed and improved.In short,the military epidemiology has made great achievements in prevention and treatment of common diseases and frequently occurring diseases,in order to guarantee the military members’ physical and mental health.In the study we review the challenges and prospects for military epidemiology.0|26|0Updated：2026-03-12
- Abstract：Objective:To provide a kinetic model（s） and reveal the mechanism of thymoquinone and Poloxin blocking an emerging anti-cancer target,human Polo-like kinase 1（hPlk1） Polo-box domain（PBD）.Methods:The binding kinetics was determined by using a fluorescence polarization based assay.The putative mechanism was examined with a competition test.Results:Thymoquinone follows a one-step binding with an association rate constant（k1） of 6.635×103 L·mol-1·min-1.Poloxin fit a two-step binding with a dissociation constant（Ki） of 118 μmol/L for the intermediate complex and its isomerization rate（k4） of 0.131 5 min-1 to form an irreversible adduct.No significant dissociation was observed for either ligand up to 13 h.The inhibitors responded insignificantly to the presence of Michael donors as hPlk1-PBD competitors.Conclusion:Thymoquinone and Poloxin are slow-tight ligands to the hPlk1-PBD with kinetic models distinct from each other.Michael addition as the mechanism is excluded.0|29|6Updated：2026-03-12
- Abstract：Objective:To observe the effect of the artesunate（ART） on cellular proliferation in vitro,to search for the possible anti-tumor mechanism of ART on endometrial carcinoma at the molecular level and to provide the experimental and theoretical foundations for the clinical applications of ART.Methods:The cell proliferation was observed by microscope;MTT was used to examine the effects of ART on proliferation of HEC-1B cells,and flow cytometric analysis was used to detect cell cycle and apoptosis.The human endometrial carcinoma HEC-1B cells were conventionally cultured;ART was administered with a concentration of 40 μg/ml before the total RNA were extracted.mRNA expression of Survivin,Caspase-3,N-Cadherin,E-Cadherin,Fibronectin1 and Cox-2 were detected using RT-PCR.Results:ART reduced proliferation in human endometrial carcinoma cell line HEC-1B in a dose-and time-dependent effect.The cells of G0/G1 stage were significantly increased（P<0.05）,but the cells of G2/M stages were significantly decreased（P<0.05）,so it has shown that the cell cycle was probably blocked in G0/G1 stage.After intervention with ART at 20 and 80 μg/ml for 48 h,cellular apoptosis rate respectively was（36.42±0.77）% and（11.77±0.58）%,and the difference was statistically significant compared with the control（[6.64±0.19]%,P<0.01）.The expression of Cox-2 mRNA in the ART group was lower than those of control group,yet the expression of Caspase-3 and E-Cadherin mRNA in the ART group was higher than those of control group.Conclusion:ART can inhibit HEC-1B cell growth and proliferation in a dose-and time-dependent manner.Furthermore,ART can induce apoptosis in a dose-dependent manner.ART is able to downregulate Cox-2 mRNA expression and to upregulate E-Cadherin and Caspase-3 mRNA expression.So we can conclude that ART could induce the endometrial carcinoma HEC-1B cell apoptosis and inhibit tumor cell proliferation.0|30|2Updated：2026-03-12
- Abstract：Objective:Placement of self expanding metallic stents（SEMS） is invariably associated with complications and often necessitates their removal usually by rigid bronchoscope.There have been few reports published on use of flexible bronchoscope（FB） for the removal.This article summarizes the indications,methods and complications of SEMS removal by FB.Methods:We reviewed our experience with removal of SEMS using FB retrospectively.The clinical data on 29 patients with average age of（39.0±13.2） years was analyzed who underwent removal of SEMS using FB between April 2002 and August 2008.Results:Thirty-seven procedures were performed in 29 consecutive patients to remove 37 stents.The average duration of stent placement was（55± 94.7） d（0-472 d）.Twenty-two procedures（59.4%） were performed under local anesthesia.The percentage of procedures under general anesthesia was 13%（3/24）,67%（4/6） and 100%（7/7） for the short-term（≤30 d）,medium-term（31-90 d） and long-term（>90 d） of stents placement,respectively.Indications for stents removal included migration in 15（40.6%）,scar restenosis in 11（29.7%）,airway shaping in 5（13.5%）,stent fracture in 2（5.4%）,stent incomplete expansion in 2（5.4%）,improper size in 1（2.7%）,mucus plugging with chest pain in 1（2.7%）.And 85.7% of long-term stents were removed due to scar restenosis.The total success rate was 92%.There was no significant difference in success rate between the uncovered and covered group（82.3% and 100%,P>0.05）.Complications were encountered in 13 patients,bleeding being the most common（53.8%）.Conclusion:Operation by FB may be an alternative method to remove SEMS effectively and safely based on the proper anesthesia chosen.0|13|0Updated：2026-03-12
- Abstract：Objective:To investigate ghrelin level change in combination with psychological stress in the hypertensive old people with cognitive impairment and to explore its effect as well as possible mechanism.Methods:The study population of 300 elders was divided into 2 groups,148 with hypertension and 152 non-hypertension,who were screened for psychological distress and cognition function,and had blood drawn to measure plasma levels of ghrelin and total cortisol on the same day.Results:The rates of anxiety and cognitive impairment were higher in the hypertension elders,which were negatively correlated with plasma ghrelin level,resulted from chronic cortisol response to anxiety.Conclusion:Chronic plasma cortisol increase to long-term anxiety leads a reduce in ghrelin level which then adversely affects blood pressure and cognitive function in old people.So measuring ghrelin of elders may be a diagnostic tool to predict cognitive development and ghrelin may be a selective antihypertensive medicine for cognitive impairment elders with or without chronic psychological stress.0|49|0Updated：2026-03-12
- Abstract：Objective:To determine whether long-term survival（>10 years） after heart transplantation is possible and identify complications influencing long-term survival.Methods:We analyzed clinical outcomes in the group of 21 patients who had undergone heart transplantation at the Second Affiliated Hospital of Harbin Medical University since 1992 and 4 of them survived more than 10 years.Results:Nine patients are still alive with normal left ventricular function,and 4 of them have survived more than 10 years.The longest survival patient has lived more than 18 years after transplantation,whose survival is the longest in China and in Asia.We have also found that there has been a high incidence of complications,such as rejection episodes during the first 6 months,and transplant vasculopathy in the long-term survival patients.Conclusion:Long-term survivors maintain normal hemodynamic function of their allografts,and long-term survival following cardiac transplantation is possible.Aggressive preventive and therapeutic measures are essential to limit the risk factors for development of complications such as rejection episodes and transplant vasculopathy,and enable long-term survival after cardiac transplantation.0|20|1Updated：2026-03-12
- Abstract：Objective:To observe the podocyte injury in diabetic nephropathy（DN） patients by identifying the urinary podocytes and the situation of detached podocytes in glomeruli and to demonstrate the correlation between podocyte excretion and proteinuria,blood glucose,serum creatinine in different phases in DN patients.Methods:Urinary podocytes and the podocalyxin（PCX） expression state of podocytes in glomeruli were identified and observed by indirect immunofluorescent method.The DN patients were divided into three groups according to the volume of proteinuria,namely small,medium and large volume proteinuria groups.The podocytes in the urine of every group were calculated.The DN patients were divided into five groups according to the chronic kidney disease（CKD） phases,then the positive podocytes in urine were calculated.Meanwhile,the 24-hour protein in urine,fasting blood glucose（FBG） and the serum creatinine of DN patients were tested.The correlations among the proteinuria,serum creatinine,FBG and the number of positive podocytes in the urine of DN patients were statistically analyzed.Results:Urinary positive podocytes were found in 88% of the patients with DN,whereas podocytes were found in 0% of patients with minimal changed disease（MCD） and healthy cases.The expression of PCX was absent in DN patients.In contrast,PCX was expressed integrally in MCD patients.The positive podocytes was 1.49±0.95/ml in small-volume proteinuria group,2.15±0.70/ml in the medium-volume proteinuria group,and 3.48±1.27/ml in the large-volume proteinuria group.There was no significant difference between the small-and medium-volume proteinuria groups,and there were significant differences between other groups（P<0.05）.The positive podocyte number tended to increase as proteinuria was increased.By Pearson analysis,the correlation between podocyte number and proteinuria was positive statistically.The difference of the number of positive podocytes in urine from different groups of DN patients,CKD Ⅰ-Ⅴ group was significant statistically.The correlation between serum creatinine of CKD Ⅰ-Ⅲ group and positive podocytes in urine was positive statistically.The correlation between serum creatinine of CKD Ⅳ-Ⅴ group and positive podocytes in urine was not significant statistically.The correlation between FBG and positive podocytes in urine was not significant either.Conclusion:The mechanism of the podocyte injury in DN patients is present.The podocyte injury in DN may positively correlate to proteinuria and serum creatinine of CKD Ⅰ-Ⅲ DN patients,but not to the FBG and serum creatinine of CKD Ⅳ-Ⅴ patients.0|37|4Updated：2026-03-12
- Abstract：Castleman disease is a rare disorder of the lymphoid tissue,characterized by lymph node enlargement with distinctive histologic features.It usually occurs within the mediastinum and rarely within the abdomen.It is usually indistinguishable from other mediastinal and hilar masses before determining a surgical approach.We present two cases of Castleman disease within the abdomen mimicking a neoplasm.0|24|1Updated：2026-03-12
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