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Volume 25 ， Issue 4 ， 2010
- Abstract：Objective:To determine the potential of sustained transgene expression by intratumoral injection of Ad-PTEN in the nude mouse model of endometrial carcinoma.Methods and Results:We constructed recombinant adenovirus carrying the wild-type PTEN gene（Ad-PTEN）.RL95-2 cells,an endometrial carcinoma cell line lacking PTEN function,was infected with Ad-PTEN and showed increased expression of PTEN and chemosensitivity to doxorubicin,decreased proliferation rate,and elevated apoptosis and G0/G1 arrest.Furthermore,the tumorigenicity of these cells was also completely suppressed.These results indicated that gene therapy with Ad-PTEN could significantly inhibit the endometrial carcinoma xenografts growth in nude mice by intratumoral injection,induce apoptosis of tumor cells,and reduce expression of proliferating cell nuclear antigen（PCNA）.Immunohistochemistry analysis also showed that the expression of progesterone receptors（PR） in Ad-PTEN treated tumor cells were induced,while P-glycoproteins（P-gp） and estrogen receptors（ER） decreased significantly.Conclusion:PTEN may play an important role in the development of endometrial carcinoma.Our findings cast new lights for treatment of endometrial carcinoma.0|13|0Updated：2026-03-12
- Abstract：Objective:To study the inhibitory effect of docetaxel（DOC）,oxaliplatin（OXA） and their combination on proliferation of cervical cancer line HeLa.Methods:Cell morphological changes were observed by inverted phase contrast microscope,cell inhibition rates in different groups were examined by MTT,and cell cycle and apoptosis rates were determined by flow cytometry（FCM）.Results:DOC and OXA inhibited the proliferation of cervical cancer cell line HeLa in a dose-dependent,and combination of the two drugs had an enhanced inhibitory effect;the apoptosis rate was also significantly increased when the two drugs were used in combination.Conclusion:DOC and OXA can synergistically inhibit the proliferation of cervical cancer cell line HeLa,which indicates that combination of the two drugs might has a promising future for clinical treatment of cervical cancer.0|31|1Updated：2026-03-12
- Abstract：Objective:To explore the efficacy of interventional therapy for chronic atherosclerotic occlusive disease of the lower extremity.Methods:Fifty-six cases of simple atherosclerotic occlusive disease of lower extremity were divided into 3 types according to the location of occlusion,including 17 cases of abdominal aorta-iliac artery,18 cases of iliac-femoral artery,and 21 cases of inferior popliteal artery.Catheter and guide wire combined with blunt dissection and subtle banding were used to dredge the occluded vessels,and stents were implanted when interlayer appeared.Results:After the treatment,46 cases of occlusion were dredged（82.1%）,6 cases remained occluded with the same symptoms（10.7%）,and 4 cases had severer symptoms（7.1%）.Among the 46 successful cases,there were 16 cases of abdominal aorta-iliac artery（94.1%）,16 cases of iliac-femoral artery（88.9%） and 14 cases of inferior popliteal artery.artery（66.6%）.There was no statistical difference between the abdominal aorta-iliac artery cases and iliac-femoral artery cases（P>0.05）,but there was statistical difference between these two types and the inferior popliteal artery cases（P<0.05）.In the successful cases,9 cases of abdominal aorta-iliac artery（52.9%）,10 cases of iliac-femoral artery（55.5%） and 10 cases of inferior popliteal artery（33.3%） had 5-10 cm of occlusion,with no statistical difference between the first two types（P>0.05）,but with statistical difference between the first two types and the third type（P<0.05）.Compared with the 14 dredged cases（71.4%）,the 7 cases（33.3%） of inferior popliteal artery cases had richer compensatory circulation（P<0.05）.Conclusion:It’s safe and effective to treat simple atherosclerotic occlusive disease of the lower extremity with interventional therapy.The location and length of occlusion and the abundance of compensatory circulation had effect on the therapy.0|12|0Updated：2026-03-12
- Abstract：Objective:To study the clinical value of Technetium-99m sestamibi myocardial perfusion single photon emission computed tomography（SPECT） in diabetes with suspected coronary artery diseases.Methods:Seventy-eight diabetic patients complicated with cardiac symptoms or electrocardiogram（ECG） abnormalities and suspected coronary heart disease hospitalized in the department of endocrinology from January,2008 to December,2008 were investigated.Technetium-99m sestamibi myocardial perfusion SPECT was performed in all patients.Exercise stress test was done in 35 cases（group A）.Adenosine stress test was done in 43 cases（group B）.Results:The positive rates in the two groups were 54.29%（19/35） and 53.48%（23/43）,respectively.There was no statistic difference between the 2 groups（P>0.05）.The abnormality rates in male and female groups were 63.04% and 28.13%,respectively（P<0.01）.Eighty-six segments of perfusion abnormality were detected in 38 positive patients.Four segments of fixed defect were found in 4 cases.Conclusion:As non-invasive method,myocardial perfusion SPECT can play an important role in the treatment of diabetes with suspected coronary artery disease.0|10|2Updated：2026-03-12
- Abstract：Three dihydrochalcones derivatives 1-3,flavone 4 and phenanthrene derivative 5 were isolated,together with 9 known compounds,from the air-dried root bark of Fissistigma bracteolatum Chatterjee.Their structures were determined by spectroscopic（NMR,MS） and chemical methodologies.0|32|2Updated：2026-03-12
- Abstract：Acute pulmonary embolism（APE） in operation room is a constant,lethal but treatable disease.A fast determination of APE in an operation room is currently difficult due to a non-specificity on its signs,symptoms,electrocardiograph findings,arterial blood gas abnormalities and chest X-rays.Clinically,an APE can automatically originate from a small and clinically asymptomatic embolus to massive,proximal emboli with shock during a scheduled surgery period.An accurate,brief and prompt diagnostic and therapeutic strategy for APE in a peri-operation period is crucial for anesthesiologists to make a decision on an appropriate clinical intervention to improve patients’ outcomes.The purpose of this mini-review article is to illuminate a fast-track diagnostic and therapeutic strategy for APE patients in a perioperative period through a hybrid of the high sensitive and high specific viable diagnostic modality of D-dimer assay in conjunction with necessary emergent pulmonary angiography（EPA）,including digital subtraction angiography（DSA）,or a spiral computed tomography angiography（sCTA） for APE patients’ diagnosis and treatment during operation.The postulated fast-track diagnostic and therapeutic strategy can effectively confirm or exclude highly suspected APE and simultaneously provide fast therapeutic opportunities for APE patients at an operative period.0|21|1Updated：2026-03-12
- Abstract：A 55-year-old man was admitted for transcatheter closure because of pulmonary arteriovenous fistula by magnetic resonance angiography（MRA）.He had a history of occasional chest pain more than one year.Angiography didn’t reveal significant stenosis at coronary artery.The patient was found a continuous grade 2/6 murmur over the left upper parastenal area one month ago.Chest MRA revealed a possible left superior pulmonary arteriovenous fistula.For diagnosis and localization of the fistula,aortography and selective angiography of the internal mammary artery was performed and presented a left internal mammary artery-to-pulmonary artery fistula.The fistula was successfully closed using an 12 mm domestic vascular plug.Chest MRA showed that the fistula disappeared at two-month follow-up.0|20|0Updated：2026-03-12
- Abstract：A 43-year-old man with a history of rheumatoid heart disease developed endocarditis.Blood culture showed endocarditis was caused by Kodamaea ohmeri and the susceptibility test showed the yeast species were susceptible to itraconazole,amphotericin B,and voriconazole,but susceptible-dose dependent to fluconazole,and resistant to 5-flucytosine.Treated with surgery and anti-fungi agents,the patient recovered from endocarditis.This is the first case of K.ohmeri fungemia found in Chinese from mainland.More and more evidence indicate that K.ohmeri is an important opportunistic pathogen for human beings.0|18|1Updated：2026-03-12
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