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Volume ， Issue 4 ， 2006
- Abstract：<正>Objective:To construct a Pichia pastoris （P. pastoris） expression vector of human intestinal trefoil factor （hITF） and study its expression and purification procedures. Methods:hITF gene encoding mature peptide was modified with a polyhistidine tag sequence at the N-terminal, and then inserted into the P. pastoris expression vector pGAPZαA at the downstream of theα-mating factor signal. After gene sequencing, the recombinant pGAPZαA-hITF was transformed into the P. pastoris strain X-33 with lithium chloride. rhITF was induced to constitutively express in shake flask, and then analyzed with Tricine SDS-PAGE and Western blotting. The obtained rhITF was isolated from the cultured supernatants by ammonium sulfate precipitation, Ni-NTA affinity chromatography, and ultrafiltration. Results:The correctness and integrity of rhITF were identified by restriction digestion and gene sequencing. rhITF was successfully expressed to 50 mg/L as a secretive protein. After purification, the purity was above 95%. Tricine SDS-PAGE and Western-blot analysis showed that rhITF presented as a single band with a molecular weight of 10 kDa, a little larger than 7 879 Da as assayed by mass spectrometry analysis. Conclusion: hITF P. pastoris expression vector is successfully constructed and rhITF is expressed in P. pastoris at commercially relevant level. This research lays foundation for the further functional studying of hITF.0|18|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the feasibility of bone marrow stromal cells （BMSCs） differentiating into cardiomyocyte-like cells in heterogeneous cardiomyocytes microenvironment in vitro. Methods: Mouse GFP-BMSCs were isolated by centrifugation through a Ficoll step gradient and purified by plating culture and depletion of the non-adherent cells. Neonatal rat cardiomyocytes （CMs） were isolated by enzymatic dissociation from hearts of 1-to 2-day-old Sprague-Dawley （SD） rats and differentially plated to remove fibroblasts. Mouse GFP-BMSCs were cocultured with neonatal rat CMs through direct and indirect contact, respectively. Cardiomyogenic differentiation of BMSCs was evaluated by immunostaining with anti -α-actin monoclonal antibody and observing synchronous contraction with adjacent CMs by phase contrast microphotography. Results: On day 7 of coculture, GFP-BMSCs （CMs : BMSCs = 4 : 1）attached to nonfluorescent contracting cells （rat-derived CMs） showed myotube-like formation and started to contract synchronously with adjacent cardiomyocytes. About 10% of the fluorescent GFP-BMSCs were cardiomy-ocyte-like cells as determined by cell morphology and positive actin staining. Conclusion:Direct cell-to-cell interaction with CMs is crucial for cardiomyogenic differentiation of BMSCs in heterogeneous CMs microenvironment in vitro. This provides a novel inducing pathway for directional differentiation of cardiovascular tissue engineering seed cells.0|7|0Updated：2026-03-12
- Abstract：<正>Objective: To explore a myogenic basis of the spontaneous contractions on the rat bladder smooth muscle strip in a detrusor instability （DI） model in vitro, and to study a nerve blocker’s cocktail affecting the spontaneous contractions as well as electrical stimulated contractile response. Methods: DI model rats were made by partial bladder outlet obstruction （BOO） and confirmed by the filling cystome-try. Detrusor strip was dissected from fresh bladder, fixed for an isometric tension trial. The contractions were recorded during electrical stimulation or exposure to some agents. Results: The cocktail diminished the nerve-mediated contractile response effectively in DI preparation. DI’s spontaneous contractions remained during the presence of the cocktail with a significant change in its contractile amplitude. Conclusion : With the local nerve-concerned factors abolishment by the cocktail, the DI bladder preparations still have the spontaneous contractions, indicating a myogenic basis from themselves.0|7|0Updated：2026-03-12
- Abstract：<正>Objective: To determine whether AQP4 expression is associated with lactate （Lac） and Nacetyl aspartate （NAA） and with apparent diffusion coefficient （ADC） abnormality after rat cerebral ischemia. Methods: The time courses of ADC and lactate and NAA assessed by proton magnetic resonance spectroscopy （HMRS） were investigated at the time point of 6 h, and 1, 3, 7 d after rat cerebral ischemia induced by middle cerebral artery occlusion. Expression of AQP4 mRNA and protein were measured using RT-PCR and Western blot analysis respectively. Results: Significant reductions of NAA concentration and increases of lactate concentration were found after rat cerebral ischemia. The expressions of AQP4 mRNA and protein were increased at 6 h, and reached the peak at 1-3 d, then began to decrease at 7 d after rat cerebral ischemia. The expression of AQP4 was significantly correlated with NAA（rRT = - 0. 856, rw = - 0.927, P<0. 01）, and with lactate （rw = 0. 473, rRT = 0. 413, P<0. 05）, and with ADC values during the period of 1-7 d after rat cerebral ischemia （rw = 0. 984, rRT= -0. 925, .P<0. 05）. In addition, correlations between Lac and the ADC values（r= - 0. 677, P<0. 05）and between NAA and ADC values during the period of 1-7 d after rat cerebral ischemia （r=0. 909, P<0. 05） were also observed. Conclusion: The data suggest that AQP4 is involved in the transport of water when brain edema is formed and cell membrane integrity is lost.0|32|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the ouabain’s effects on the ultrastructure and function of the rat heart. Methods: Male Sprague-Dawley （SD） rats were treated with ouabain and systolic blood pressure （SBP） were recorded weekly. After 4 weeks, echocardiography was performed, hemodynamic parameters were measured by invasive cardiac catheterization and changes in heart ultrastructure were analyzed using transmission electron microscopy. Results:After treated by ouabain for 4 weeks, there were no significant differences in the mean SBP of the two groups. However, cardiac systolic and diastolic performances were both worsened with ouabain treatment by echocardiography, left ventricular chamber diameters and wall thickness were significantly increased in the rats of ouabain group. Invasive monitoring indicated that left ventricular systolic pressures （LVSP）, rate of pressure development （+dp/dt） and rate of pressure decay （-dp/dt） were significantly attenuated and left ventricular end-diastolic pressures （LVEDP） were increased in ouabain group （P<0. 05）. Disorganization of myofilaments, mitochondrial swelling, disruption and vacuolation, hyperplastic collagen fibers were found in ouabain group by transmission electron microscopy. Conclusion:It is suggested that ouabain induces alterations in cardiac ultrastructure and function, and the effects happened before the increase of blood pressure, which indicates that ouabain might damage rat heart independent of blood pressure.0|13|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the effects and mechanisms of interferon in combination with all-trans retinoic acid （ATRA） on proliferation and differentiation of ATRA-resistent APL cell. Methods:After MR2 cells （ATRA-resistance cell line） were treated with IFN-α, IFN-γand ATRA alone or IFN-αand IFN-γin combination with ATRA respectively. The cell proliferation was tested by MTT test and the cell differentiation was tested through light microscope by NBT test and flow cytometry （FCM）. The expression of promyelocytic leukemia （ PML） protein was observed by indirect immune fluorescent method. Results: Both IFN-αand IFN-γcould inhibit the proliferation and induce the differentiation of MR2 cells to some extent. The effects were more obvious after both interferons in combination with ATRA respectively （P<0. 05）. Moreover, the maturation of MR2 cells induced by IFN-γ+ ATRA group was more higher than that by IFN-α+ ATRA group （P<0. 05）. Both interferons could induce the expressions of PML protein. Conclusion:Both interferons can inhibit MR2 cells proliferation, which may be related to the expression of PML protein induced by both interferons. The inducing differentiation effects of IFN-γ+ ATRA group on MR2 cells are more powerful than those of IFN-α+ATRA group, which may be related to the different signal transduction pathway of both interferons.0|32|0Updated：2026-03-12
- Abstract：<正>Objective:To examine CT and pathologic presentations of the periphery of hepatocelllar carcinoma （HCC） by using in vivo liver specimens from orthotopic liver transplantation （OLT） and to see whether there is any correlation between them. Materials and Methods:Forty-two patients with HCC confirmed by pathology and liver transplantation were included. Plain CT scan covering the whole liver was done, followed by arterial and portal venous phase scanning. The delay time was 25 s and 60 s after injection of contrast medium. Fresh liver samples were evaluated, sliced and stained with HE and the standard immunoperoxidase method using facterⅧrelated antigen （F8RA） monoclonal antibody to study CT presentation of the periphery of tumor and compare them with pathologic findings. Results: （1） Tumors with clear boundaries on CT scan reflected the presence of tumor capsules in pathologic sections. Most tumors with unclear boundaries had no capsules and grew in an invasive pattern; （2） Tumors with unclear boundaries or high density on dynamic enhancement usually had abundant newborn vessels in the periphery and were poorly differentiated, while those with low density had few newborn vessels and were well differentiated; （3） When microvessel density （MVD） was>30, the occurrence of intrahepatic daughter foci was higher. Conclusion: （1） CT morphologic classification of the HCC periphery can well reflect the intactness of tumor capsules, which is helpful for preoperative assessment of tumor infiltration and cancer cell grading; （2） CT enhancement presentation of the HCC periphery in arterial and portal phases may reflect pathologic changes and help predict the prognosis at large.0|5|0Updated：2026-03-12
- Abstract：<正>Objective: To investigate the enhancement basis and the mechanisms of solitary pulmonary nodules （SPNs） by comparing the differences in microvascular structure between benign and malignant lesions. Methods: Dynamic contrast-enhanced CT scan was performed on 53 patients with SPNs （diameter<3 cm. 38 peripheral lung cancers, 5 hamartomas, 10 inflammatory lesions） using a Siemens Plus S or a Marconi MX8000 multi-slices spiral CT scanner. The time-attenuation curves were interpreted. The microvascular density （MVD） and the continuity of the microvessels’ basemental membrane in the dissected specimens were observed with the ABC （avidin-biotin-complex） immuno-histochemical method in all patients. ResultS:The CT enhancement values of lung cancer （49. 05±16. 08 HU） and inflammatory lesions （49. 59±21. 30 HU） were significantly higher than those of hamartoma （8. 98±4. 56 HU） （t = 7. 48. P<0. 05; t = 8. 35, P<0. 05）. But the enhancement of lung cancer was similar to that of inflammatory lesions （t = 0. 76. P>0. 05）. The time-attenuation curve of inflammatory lesions tended to increase faster and reached a higher peak compared to the lung cancer, and both of them maintained a high plateau after crossing. The hamartoma showed a slight increase in the time-attenuation curve and demonstrated a low-plateau curve. The MVD of SPNs was positively correlated with CT enhancement （r=0. 8051）. The microvascular counts of peripheral lung cancer （48. 45±10. 09） and inflammatory lesions （19. 60±19. 94） were significantly higher than those of hamartoma （8.70±7.30） （t = 11.64, P<0.001;t = 6. 09. P< 0. 001）. but no significant difference was found between lung cancer and inflammatory lesions （t= -0. 26, P = （）. 799）. There was no difference in the continuity of basement membrane between nodules with an enhancement less than 30 HU and those with an enhancement higher than 30HU （x2 = 3. 13, P>0. 05 ）. Conclusion: The microvascular counts mainly contribute to the enhancement value of SPNs. The basement membrane is not related to nodule enhancement, but it might influence the pattern of the time-attenuation curve.0|13|0Updated：2026-03-12
- Abstract：<正>Objective: To discuss imaging features of the adrenal glands in female pseudo-hermaphroditism. Methods: 11 cases of female pseudohermaphroditism （8-27 years old） were analyzed retrospectively. Results: In 9 of the 11 patients with female pseudohermaphroditism who did no receive hormone replacement therapy, both internal and external branches of bilateral adrenal glands were found to be thickened, prolonged and twisted, and in 2 of the 9 patients they were found to be macronodularly hyperplasic. In one of the remaining two patients who had received long-term hormone replacement therapy, the adrenal glands were not thickened or twisted, though prolonged; and in the other patient imaging presentation of the adrenal glands was the same as that of those who did not receive hormone replacement therapy, but with co-existence of adrenal myelolipoma. Among the 11 patients agenesis of the uterus and the vagina was found in 4 cases. Conclusion: Female pseudohermaphroditism is a hereditary disease, where hyperplasia of the adrenal glands and agenesis of the uterus and the vagina were secondary. Early detection of these abnormalities by imageology would prove to be helpful in early detection and treatment of the condition.0|16|1Updated：2026-03-12
- Abstract：<正>Objective: Using receiver operating characteristics （ROC） curve to evaluate the value of pulse wave velocity （PWV） in the diagnosis of coronary heart disease （CHD）. Methods: By using coronary angiography as golden diagnostic standard of CHD, 218 patients were divided into both CHD group （n=121） and non-CHD group （n = 97）. All these patients received PWV test. The efficacy of PWV of each artery segments in the diagnosis of CHD was evaluated by ROC curve. The sensitivity and specificity were calculated with the golden diagnostic standard of CHD. Results:The PWV of right carotid to femoral artery （Rc-f）, left carotid to femoral artery （Lc-f）, right radial to carotid artery （Rc-r）, left radial to carotid artery （Lc-r） in CHD group were significantly higher than that of non-CHD group （9. 31±1. 75 vs 7.60±1.59, P<0. 01; 9. 02±1.71 vs 7. 52±1.50, P<0. 01; 8. 69±1. 37 vs 8. 00±1. 27, P<0. 01; 8.52±1. 03 vs 8. 03±1. 2, P<0. 01 respectively）. However, the PWV of both right and left femoral to ankle artery （Rf-a and Lf-a） had no significant differences between the two groups. We then compared the area under curve （AUC） of each ROC（AUCROC） of PWV of Rc-f, Lc-f Rc-r and Lc-r to evaluate their diagnostic efficacy for CHD. We found that AUCROC of Rc-f PWV was the biggest （AUCROC = 0. 818）, at the peak point of its ROC curve, the PWV was 8. 32 m/s. PWV>8. 32 m/s of Rc-f could predict the presence of CHD with a sensitivity of 79% and specificity of 77%. Conclusion: The PWV of Rc-f, Lc-f, Rc-r, Lc-r are significantly higher in CHD group than that in non-CHD group, and PWV of Rc-f is the most accurate in the detection of CHD. The PWV>8. 32 m/s of RC-F is a valuable predictor of CHD.0|26|3Updated：2026-03-12
- Abstract：<正>Objective:To investigate the effect of injecting a compound of Bupivacaine and Fentanyl into sacral spinal space to treat chronic pelvic pain syndrome （CPPS）. Methods: A total of 36 men with recalcitrant CPPS refractory to multiple prior therapies were treated with the injection of a compound of Bupivacaine and Fentanyl （10 ml of 0. 125% Bupivacaine, 0. 05 mg Fentanyl, 5 mg Dexamethasone, 100 mg Vitamin B1 and 1 mg Vitamin B12） into sacral space once a week for 4 weeks. The National Institute of Health Chronic Proslatitis Symptom Index （NIH-CPSI）, maximum and average flow rate were performed at the start and the end of 4 weeks’ therapy. Results :Mean NIH-CPSI total score was decreased from 26. 5±1. 6 to 13. 4±2. 0 （P<0. 001）. Significant improvement was seen in each subscore domain. A total of 32 patients （89%） had at least 25% improvement on NIH-CPSI and 22 （61 %） had at least 50% improvement. Maximal and average flow rate were increased from 19. 5±3. 8 to 23. 6±4. 2 and 10. 9±2. 6 to 14. 3±2. 4 respectively. Conclusion: Injection of this compound of Bupivacaine, Fentanyl and Dexamethasone into sacral spinal space is an effective and safe approach for recalcitrant CPPS. Further study of the mechanisms and prospective placebo controlled trials are warranted.0|6|2Updated：2026-03-12
- Abstract：<正>Objective:To retrospectively compare the efficacy and safety of ultrasound-guided thrombin injection （UGTI） with ultrasound-guided compression repair （UGCR） in patients with postcatheter-izational femoral arterial pseudoaneurysms （PSA）. Methods: Thirty patients of this iatrogenic PSA [8 males, 22 females, average age （66. 5±5. 2） years] in our institution from 1997 to 2004 were retrospectively analyzed. Among them, 11 patients were treated with UGCR, 2 under continuous ultrasonographic （US） guidance and 9 under the guidance of femoral arterial bruit auscultation and dorsalis pedis artery palpation. Because UGCR was failed in 5 patients, consecutively 24 patients were treated with UGTI. Swine thrombin solution at a concentration of 200 U/ml was injected percutaneously using 22-25 gauge needles under color Doppler US. Demographics, clinical variables, pseudoaneurysm characteristics, and results of the 2 groups were compared by using Fisher’s exact test and Student’s t test. Results: The initial success rate of UGCR was 36. 4% （4/11） and the overall success rate was 45. 5% （5/11）. Ten of 11 patients suffered from local pain during the compression, but there was no any complication in UGTI group. The average dose of injected thrombin was （180 + 82） U for PSA of a single loculus and （315±150） U for multiloculated PSA. The initial success rate of UGTI was 89.5% （17/19） and the overall success rate was 100% （24/24）. Conclusion;UGTI offers a safe, quick and effective means of definitively treating femoral pseudoaneurysms and seems superior to UGCR. The amount of thrombin applied on our people seems smaller compared with others’ work.0|22|3Updated：2026-03-12
- Abstract：<正>Objective:To search for risk factors that affect mother-to-infant transmission of hepatitis B virus （HBV）. Methods: To obtain studies eligible for meta-analysis, China biological medicine discs and MEDLINE citations were surveyed. Mother HBV DNA or HBeAg positivity, neonate HBeAg positivity, mode of delivery, threatened abortion and threatened premature labor were processed with meta analysis. Criteria for selection of published studies for meta analysis were based on principle by Abdolmaleky HM[1]. Odds ratio （OR） was calculated and summarized by fixed effect model or random-effects model using RevMan software. The heterogeneity of the group of ORs was assessed using an x2 test. The significance of the pooled OR was determined by the u-test. The strength of association was assessed using the OR. An OR>1. 0 indicated a positive association between the risk factor and neonate HBV infection. Results: After meta analysis of factors concerned, a significant association was found between the positivity of HBeAg in mother and neonate, of HBV DNA in mother peripheral serum, and HBV mother-to-infant transmission, with a pooled OR equal to 19.43 （95% CI=8. 77-43. 06）, 36.5 （95% 01=19.85-67.11）, and 36. 5 （95% CI=19. 85-67. 11） respectively. Mode of delivery, threatened abortion and threatened premature labor proved not to be of risk factors on the mother-to-infant transmission of HBV. Conclusion: Mother HBV DNA or HBeAg positivity and neonate HBeAg positivity were proved to be of risk factors affecting the transmission of HBV from mother to fetal.0|46|2Updated：2026-03-12
- Abstract：<正>Hemangiomas of the gastrointestinal tract are uncommon, accounting for only 0. 05% of all intestinal neoplasm. Bleeding, chronic anemia, and abdominal pain are the most common presenting features. We report a case of small bowel multiple cavernous hemangioma presenting as gastrointestinal tract bleeding.0|8|0Updated：2026-03-12
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