<正>Objective: To investigate the effects and mechanisms of interferon in combination with all-trans retinoic acid （ATRA） on proliferation and differentiation of ATRA-resistent APL cell. Methods:After MR2 cells （ATRA-resistance cell line） were treated with IFN-α

IFN-γand ATRA alone or IFN-αand IFN-γin combination with ATRA respectively. The cell proliferation was tested by MTT test and the cell differentiation was tested through light microscope by NBT test and flow cytometry （FCM）. The expression of promyelocytic leukemia （ PML） protein was observed by indirect immune fluorescent method. Results: Both IFN-αand IFN-γcould inhibit the proliferation and induce the differentiation of MR2 cells to some extent. The effects were more obvious after both interferons in combination with ATRA respectively （P<0. 05）. Moreover

the maturation of MR2 cells induced by IFN-γ+ ATRA group was more higher than that by IFN-α+ ATRA group （P<0. 05）. Both interferons could induce the expressions of PML protein. Conclusion:Both interferons can inhibit MR2 cells proliferation

which may be related to the expression of PML protein induced by both interferons. The inducing differentiation effects of IFN-γ+ ATRA group on MR2 cells are more powerful than those of IFN-α+ATRA group

which may be related to the different signal transduction pathway of both interferons.