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Volume ， Issue 5 ， 2004
- Abstract：<正>Objective: To investigate the mitochondrial DNA sequence polymorphism in Chinese Dongxiang ethnic group and to provide basic data used in ethnic origin investigation and forensic purpose. Methods: Genomic DNA was extracted from the whole blood of 100 unrelated individuals of Chinese Dongxiang ethnic group by standard Chelex-100 method. The sequence polymorphism was determined by PCR amplification and direct sequencing. Results: Eighty-two polymorphic sites were identified in mtDNA D-loop region 16 091 - 16 418 np, and 88 haplotypes were found. The genetic diversity was calculated to he 0.9969, and the genetic identity was 0.013 2. Conclusion: There are some particular polymorphic sites in Chinese Dongxiang ethnic group, and these sites provide an important basis to investigate the origin of Dongxiang and the relationship between Dongxiang and other ethnic groups. The result also suggested that sequence polymorphism from 16 091 -16 418 np in human mitochondrial DNA control region can be an useful tool for0|36|0Updated：2026-03-12
- Abstract：<正>Objective: To study the effects of hyperbaric air exposure on the functions of peritoneal macrophages of mice. Methods: Forty-eight mice were equally randomized to 6 groups: （1） normal air group （NA）; （2） hyperbaric air group 1 （HA1）; （3） hyperbaric air group 2 （HA2）; （4） hyperbaric air group 3 （HA3）; （5） hyperbaric oxygen group （HO）;（6） hyperbaric nitrogen group （HN）. Every group was exposed to corresponding pressure for 60 min, twice a day for 3 d. Peritoneal macrophages were obtained at the corresponding time to observe the changes of phagocytosis, acid phos-phatase, antigen presentation function and the produce of NO and TNF-α. Results: Compared with those in NA group, the activity of phagocytosis, acid phosphatase, antigen presentation function and the produce of NO and TNF-a were markedly inhibited in hyperbaric oxygen group and hyperbaric air group 1 （ P < 0.05, P < 0.01） and they changed little in HN group. These changes could disappear in 3 - 5 d. Conclusion: The functions of mice peritoneal ma0|15|0Updated：2026-03-12
- Abstract：<正>Objective: To estimate directly phagocytic functions of the macrophages because of the importance in innate immunity, determine blood vessel density and re-innervation density which are basis of function. Methods: Eighty adult Wistar rats were randonjy divided into experimental and control groups.The formers underwent splenotomy and a half splenie slice was transplanted into greater omentum. The latter only moved. After 6 months, examination was made as follows: ① After injection of 0.4% carbon particles by vein, spleme tissues were taken out at different times for estimating phagocytosis by light microscope. ② When splenic tissues had been intubated into left ventricle under total anesthesia, animals were perfused by formalin and India ink mixture suspension. Splenic tissues were taken out for making sections for measurement of area density of blood vessels.③ Immunohistochemical procedure was used for detecting neuropeptide Y（NPY）. Results: Phagocytie functions had no difference between two groups, h0|11|0Updated：2026-03-12
- Abstract：<正>Objective: To observe the damage of mixed reflux to the rat esophageal mucosa, and investigate the preventive effects of cisapride, nabumetone and hydrotalcite on the damaged esophageal mucosa. Methods: Three hundred sixty-eight Sprague-Dawley rats were treated as esophagoduodenostomy and divided into four groups in random. Group Y:operation + saline as positive controls; Group P: operation + cisapride; Group R: operation + nabumetone; Group D: operation + hydrotalcite. Different drugs were perfused in the 1st week after operation. The lesions of esophageal mucosa were observed in the 5th, 9th, 13th, 17th, 22nd, 28th, 35th and 40th week respectively, and evaluated the preventive effects of these drugs. Results: The lesions of esophageal mucosa in group Y were more severe than other three groups in different time （ P < 0.05）, and the incidence of Barrett’ s esophagus（BE）, severe atypical hyperplasia and esophageal adenocarcino-ma （EAC） in group Y were higher than others. After 22 weeks, the lesions in gro0|28|1Updated：2026-03-12
- Abstract：<正>Objective: To investigate the role of spinal glial cells activation in neuropathic pain in a recently developed spared nerve injury （SNI） animal model by Decosterd and Woolf. Methods: A lesion was made to two of the three terminal branches of the sciatic nerve of rats （tibial and common peroneal nerves） leaving the sural nerve intact. Continuous intrathe-cal administration of propentofylline, a glial modulating agent, 1 d before and 5 d after operation, was performed to disrupt spinal cord glia function. The vehicle was intrathecally administrated as control. The paw withdrawal threshold to mechanical stimulation （paw withdrawal mechaical threshold PWMT）, body mass and motor function were determined pre- and post-surgery. Results: It produced a prolonged mechanical allodynia in the medial and lateral part of the ipsilateral hind paw in SNL models. The treatment with propentofylline significantly prevented the development of mechanical allodynia located in either medial or lateral plantar surface. Rats in0|18|1Updated：2026-03-12
- Abstract：<正>Objective: To observe the changes of mu and kappa opioid receptors in the cathartic colon of rat, and to clarify that whether opioid receptors accounts for the occurrence of slow transit constipation （STC）. Methods: The cathartic colon model of rat was made by feeding with laxatives. The activity of mu and kappa opioid receptors in the cathartic colon of rat was measured by radio-ligand binding assay. Results: Compared with the control group, the maximal binding capacity （Bmax） and affinity（Kd） of mu opioid receptor in cathartic colon group were significantly increased （207.00±22.90 fmol/mg·p vs 82.00±14.23 fmol/mg·p, P < 0.01;3.30±0.45 mmol/L vs 2.40±0.57 mmol/L,P < 0.05）. The maximal binding capacity of kappa opioid receptor also showed a great increase （957.00±102.41 fmol/mg·p vs 459.00±52.41 fmol/mg·p, P<0.01）, but no significant difference of affinity was found between the two groups. Conclusion: The mu and kappa opioid receptors may be involved in the functional disorders of cathartic col0|18|0Updated：2026-03-12
- Abstract：<正>Objective: To construct retroviral vector with HBV Pol gene and study its expression in vitro. Methods: The recombinant plasmid HBV2/pBR322 containing 2 copies of HBV full-length gene was provided as the amplification template. The PCR product was cloned into pMD 18-T and subcloned into pMSCVneo and pLNCX2 to construct the recombinant retroviral vectors with HBV Pol gene named by HBV P/pMSCVneo and HBV P/pLNCX2. HBV Pol gene was detected by RT-PCR after transfecting the recombinant plasmids into SMMC7721 cells with liposome and G418 selection. Results; The retro-viral vector with HBV Pol gene was successfully constructed and the expression of HBV Pol gene in vitro was detected by RT-PCR. Conclusion: The retroviral vector with HBV Pol gene can be obtained, which will provide a new insight on the function of HBV Pol gene.0|18|1Updated：2026-03-12
- Abstract：<正>Objective: To study the effects of Salvia Miltiorrhiza Bge （SMB） on myocardial fibrosis in hypertension in rats. Methods: Normal Wistar kyoto rats served as negative control （group A） . The rats with hypertension were divided into 2 groups: the hypertension group （group B） which served as the positive control and the SMB-treated hypertension group （group C）. The effects of SMB on the systolic pressure, the myocardial content of aldosterone （ALD） and nitric oxide （NO） and the activity of superoxide dismutase （SOD） in the myocardium were detected. Results: The systolic pressure of the rats of group C showed no marked change after the administration of SMB and showed no difference from that of the rats of group B. The content of collagen Ⅰ and Ⅲ of the left ventricle, the collagen Ⅰ /collagen Ⅲ ratio and the content-of ALD were lower in group C than in group B and higher in group C than in group A. The content of NO and the activity of SOD in the myocardium were higher in group C than in group B and low0|16|1Updated：2026-03-12
- Abstract：<正>Objective: To study the expression of PKD2 gene in human kidney and other tissues. Methods: The expression of PKD2 was detected by reverse transcription PCR（RT-PCR） and in situ hybridization（ISH） . The results of ISH were analyzed by micromegakargooytes. Results: Distribution of pkd-2 in normal adult kidney was stronger in proximal convoluted tubule, Henle’s loop ascending branch, distal convoluted tubule and cortical collecting ducts, and inferior signal were observed in fetal kidney. Negative was seen in ADPKD 2 kidney. Conclusion: Down-regulation of PKD2 gene expression in kidney may take effect on the occurrence and development of ADPKD2.0|28|0Updated：2026-03-12
- Abstract：<正>Objective: To evaluate the incidence and pattern of rhodopsin （RHO） mutations in Chinese patients with retinitis pigmentosa （RP）. Methods: Conformation sensitive gel electrophoresis （CSGE） and direct DNA sequencing were applied to detect point mutations that occurred in the five coding exons and splice sites of RHO gene in 98 index patients with RP. Results: Four patients of one ADRP family were found to have a missense mutation at codon 347, Pro347Leu. One late-onset RP patient and her daughter, without clinical expression at present, were discovered to have a novel frameshift mutation at codon 327, Pro327 （1-bp del） . Neither of the two mutations was found in 100 normal controls. Ala299Ser was found in one RP patient. Two control subjects also had Ala299Ser, suggesting its nonpathogenicity and just single nucleotide polymorphism （SNP）. Conclusion: Two RP patients had rhodopsin mutations, thus the expected frequency of RHO mutations in RP is about 2.0% （95% confidence interval: 0.3%-4.4%）. A highly cons0|13|0Updated：2026-03-12
- Abstract：<正>Background: Recent data suggest that beta-blockers can be beneficial in patients with chronic heart failure （CHF）. Atrial fibrillation （AF） is present in a significant number of patients with CHF and is associateing with significant morbidity and increasing mortality rates. Thus it is necessary to establish therapy to improve the poor prognosis in this high-risk population, but a specific benefit of beta-blockers to the subset with concomitant AF and CHF has been little demonstrated. Objective: To examine the effects of Bisoprolol （6 months treatment） on the ventricular function and hemodynamics in patients with AF and CHF. Methods: 84 patients with stable CHF （NYHA≤Ⅲ class） and AF were assigned to Treated Group（ n = 37） or Control group Ⅰ （ n = 22, 24-hour heart mean rate < 70/min） or Control Group Ⅱ （ n = 25, 24-hour heart mean rate ≥ 70/min） . All patients were given the basic therapy for CHF, and Treated Group received Bisopolol. Clinical and echocardiographic variables were measured in 3 groups0|9|0Updated：2026-03-12
- Abstract：<正>Objective: To explore the pathogenesis of trigeminal neuralgia （TN） and to provide a new target for the drug treatment of TN by studying the expression of tetrodotoxin-resistant hNav1. 8 sodium channel protein in affected nerves of patients with TN. Methods: Twelve affected inferior alveolar nerves were obtained from patients with idiopathic TN, to whom the drug therapy was not effective. As negative control, one nonnal inferior alveolar nerve was obtained from patients who accepted the combined radical neck dissection with glossectomy and mandibulectomy. One muscle sample was obtained as normal control. One dorsal root ganglion from rat was as positive control. These tissues and prepared hNav1. 8 antibody were conducted immunohistochemistry response. Results: hNav1.8 channel protein was expresses in all the 12 specimens of the affected nerves of patients with TN, but not in the muscle sample and the normal inferior alveolar nerve. Conclusion: The abnormal expression of hNav1. 8 channel protein in the af0|10|0Updated：2026-03-12
- Abstract：<正>Objective: To study the composition of microecosystem in ostiomeatal complex （OMC） of adults and the changes via functional endoscopic sinus surgery. Methods: 220 adults with chronic sinusitis（20 as control） were classified into pre- and post-operative groups, and categorised according to its type of sinusitis. Swab specimens were taken from three different sites of ostiomeatal complex in each individual, and cultivations and identifications of aerobes, anaerobes and fungi were performed simultaneously. The microecosystem was analysed. Results: Cultured aerobes belonged to 30 species from 13 genera, anaerobes species, and fungi 3 major species, most of which have none or less virulence. The difference of mi-croecological status between type Ⅰ and Ⅱ was not significant （P > 0.05）. Aerobic culture rates between preoperative group and control group had significance（P < 0.01）;culture rate descended in postoperative group while the difference was not significant compared to preoperative group （P > 0. 05）; d0|6|1Updated：2026-03-12
- Abstract：<正>Objective: To study the effective computerized image processing of underexposed and overexposed X-rays of bones and joints. Methods: Ninety-nine conventional X-ray images （82 were overexposed and 17 were underexposed）, scanned by an UMAX Astra 4000U Scanner, were converted into digital images on the basis of their analog images. A computerized imaging processing program consisting of five functional modules such as Contrast Stretch, Fast Flourier Transform （FFT）, Image Smoothing Modules, Inverse Fast Flourier Transform （IFFT） and Nonlinear Transform performed image contrast stretch and smoothing. Three senior doctors from hospital image sections made their evaluation of all the processed images. Results:Of 82 overexposed films, 71 met the clinical requirements after image processing, and 11 were unable to be applied to clinical diagnosis, accounting for 87% and 13% respectively. Of the other 17 underexposed X-ray images, 11 met the clinical requirements while 6 were not, making a percentage of 64 and 35.0|23|1Updated：2026-03-12
- Abstract：<正>Primary yolk sac tumor of the mediastinum is very rare. Yolk sac tumor is highly malignant, and has less chance of operation. One case, which treated in this institute since 1954, is retrospectively studied to investigate the clinical characteristics, surgical treatment and prognosis of the primary mediastinal yolk sac tumor.0|15|0Updated：2026-03-12
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