Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats
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Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats
Military Medical ResearchIssue 5, Pages: 267-271(2004)
Affiliations:
1. Department of Applied Surgical Anatomy and Operative Surgery Third Military Medical University
2. ,China
Author bio:
Funds:
DOI:
CLC:R392
Published:2004
Accepted:
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[1]蒋登金,郭光金,张坤,兰阳军,王林,张天飞,左艳芳.Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats[J].Journal of Medical Colleges of PLA,2004(05):267-271.
DOI:
[1]蒋登金,郭光金,张坤,兰阳军,王林,张天飞,左艳芳.Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats[J].Journal of Medical Colleges of PLA,2004(05):267-271.DOI:
Determination of phagocytic functions of macrophages, vascular density and NPY-nerve within autotransplanted splenic tissue in adult rats
摘要
Abstract
<正>Objective: To estimate directly phagocytic functions of the macrophages because of the importance in innate immunity
determine blood vessel density and re-innervation density which are basis of function. Methods: Eighty adult Wistar rats were randonjy divided into experimental and control groups.The formers underwent splenotomy and a half splenie slice was transplanted into greater omentum. The latter only moved. After 6 months
examination was made as follows: ① After injection of 0.4% carbon particles by vein
spleme tissues were taken out at different times for estimating phagocytosis by light microscope. ② When splenic tissues had been intubated into left ventricle under total anesthesia
animals were perfused by formalin and India ink mixture suspension. Splenic tissues were taken out for making sections for measurement of area density of blood vessels.③ Immunohistochemical procedure was used for detecting neuropeptide Y(NPY). Results: Phagocytie functions had no difference between two groups
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