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Volume ， Issue 4 ， 2008
- Abstract：Objective: To elucidate distinct functions of a recently identified cancer metastasis-associated molecule, related to testes-specific, vespid, and pathogenesis protein-1 （RTVP-1） in the mammalian immune system. Methods: Immunohistochemical assays and functional analysis on the immune system were performed on RTVP-/- mice and RTVP-1+/+ mice. Protein-protein interaction （PPI） was used to predict the functions of RTVP-1. Results: Abnormal lymphocyte growth kinetics and reduced numbers of CD8+ T cells were revealed in lymph nodes of RTVP-1-/- mice. Expression of phenotypic markers of maturation in bone marrow-derived dendritic cells （DC） was impaired in RTVP-1-/- DC following antigenic stimulation in vitro and RTVP-1-/- DC failed to provide normal CD4+ T cell stimulatory activities in vivo. RTVP-1-/- mice failed to generate normal CTL or antibody responses in vivo after vaccination. In vivo tumor challenge experiments using a mouse cancer cell line demonstrated that the growth rate of subcutaneous tumors in RTVP-1-/- mice was significantly increased and CD8+ T cell infiltration significantly reduced compared to RTVP-1+/+ mice. PPI showed that RTVP-1 protein closely interacted with molecules associated with immune response and cancer metastasis. Conclusion: RTVP-1 might function as a tumor metastasis suppressor and immunosurveillance molecule in cancer.0|12|0Updated：2026-03-12
- Abstract：Objective: To explore the impact of s-phase kinase-associated protein 2 （Skp2） on cervical cancer cell proliferation and the relationship between Skp2 and expression of cell regulation factors and transcription factors. Methods: RNAi technology was used to silence Skp2 gene in HeLa cells. After interference, RT-PCR was used for detection of Skp-2 mRNA, and Western blotting and flow cytometry were used for protein expression analysis. Results: siRNA significantly inhibited HeLa cell proliferation （P<0.05） and increased HeLa apoptosis, and G1/G0 phase cells were increased significantly （P<0.01）. Skp2 siRNA transfected HeLa cells effectively reduced Skp2 protein levels, while p27 and p-p53 protein levels were increased significantly. RT-PCR results showed that after interference Skp2 mRNA, c-myc mRNA and cyclin A mRNA expressions decreased significantly compared with those in control group （P<0.01）, and p27mRNA expression level was significantly higher （P<0.01）. Conclusion: The change of Skp2 expression affects the expression of the cell cycle protein, thus affecting proliferation and apoptosis of HeLa cells. Skp2 protein plays an important role in the progression of cervical cancer; yet the specific mechanism still needs further study.0|33|4Updated：2026-03-12
- Abstract：Objective: To investigate in vitro differentiation of oligodendrocyte precursor cells （OPCs） into mature oligodendrocytes in chemical conditional medium. Methods: The mixed glial cells from cerebral cortices of 48-hour-old Sprague-Dawley （SD） rats were cultured in vitro. The OPCs were separated by shaking procedure around 9–10 d in the primary culture. Then the isolated OPCs were further transferred into the chemical conditional medium for cell differentiation. The pattern of OPCs maturation in vitro was continuously observed with contrast phase microscopy and mature oligodendrocytes were further identified by immunocytochemical assays. Results: OPCs grew well when co-cultured with glial cells and distinct cellular stratification formed about 9–10 d in the primary culture, which indicated the appropriate opportunity for the separation of OPCs. Following cultured in the chemical conditional medium, the OPCs progressively differentiated into the mature oligodendrocytes. These mature oligodendrocytes were also immunostained with the oligodendrocyte lineage-specific antibody, Oligo2. Conclusion: The OPCs isolated from the cerebral cortices of neonatal SD rats can progressively differentiate into mature oligodendrocytes in the chemical conditional medium in vitro.0|14|0Updated：2026-03-12
- Abstract：Objective: To design and test the accuracy and efficiency of our lung segmentation algorithm on thoracic CT image in computer-aided diagnostic （CAD） system, especially on the segmentation between left and right lungs. Methods: We put forward the base frame of our lung segmentation firstly. Then, using optimal thresholding and mathematical morphologic methods, we acquired the rough image of lung segmentation. Finally, we presented a fast self-fit segmentation refinement algorithm, adapting to the unsuccessful left-right lung segmentation of thredsholding. Then our algorithm was used to CT scan images of 30 patients and the results were compared with those made by experts. Results: Experiments on clinical 2-D pulmonary images showed the results of our algorithm were very close to the expert’s manual outlines, and it was very effective for the separation of left and right lungs with a successful segmentation ratio 94.8%. Conclusion: It is a practicable fast lung segmentation algorithm for CAD system on thoracic CT image.0|26|4Updated：2026-03-12
- Abstract：After interface layer was simulated by the magnetic nano-particles in the egg white phantom, high intensity focused ultrasound （HIFU） at the same dosage was introduced to radiate the phantom in different depths to blow the acoustic interface layer to mimic "point" exposure. The results showed that the volumes of biological focal region （BFR） were enlarged when the acoustic focal region （AFR） is close with interface layer. This meant that the magnetic nano-particles enhanced the therapeutic efficiency of HIFU. When the distance of the AFR from the interface layer was 10 mm, the size and shape of the BFR were similar with those of the control group, but a larger lesion at the interface, which was harmful for treatment, was observed. When the distance of the AFR to the interface layer increased to 30 mm, the size and shape of the BFR were also similar to those of the control group. When the thickness of the interface layer diminished, the utility of enhancement decreased. Continuous increase of the safe area for treatment and decrease of the utility of enhancement were observed along with the abatement of the thickness of the interface layer0|19|1Updated：2026-03-12
- Abstract：Background: The carcinogenesis of hepatocellular carcinoma （HCC） is a multi-factorial, multistep and complex process. Its prognosis is poor and early detection is of the utmost importance. Transforming growth factor β1 （TGF-β1） message RNA （mRNA） has been reported to be elevated in HCC patients using Northern blotting. However, little work has been done about the detection of TGF-β1 mRNA levels in peripheral blood of patients with HCC using the real-time polymerase chain reactions （PCR） method. Objective: To assess the prognostic value of quantitative levels of TGF-β1 mRNA in peripheral blood of patients with HCC, and to investigate the relationship between the expression of TGF-β1 mRNA in peripheral blood and many diagnostic and pathological factors. Methods: We developed an optimized Taqman real-time PCR to quantify TGF-β1 mRNA in peripheral blood of 53 patients with HCC and 44 healthy volunteers. In addition, blood was collected from patients with HCC for measuring levels of total bilirubin （TBil）, prealbumin, albumin, alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, gamma glutamyltranspeptidase （GGT）, alpha-L-fucosidase （AFU）, alpha fetoprotein （AFP）, carcino-embryonic antigen （CEA）, carbohydrate antigen 19-9 （CA19-9）, viral load and platelet counts. Statistical analysis was performed using the SPSS software system （SPSS 10.0）. Results: In real-time PCR, fluorescence was detectable in all blood specimens from patients with HCC and healthy volunteers. The levels of TGF-β1 mRNA expression in patients with HCC were significantly higher compared to that in healthy volunteers （P<0.000 1）, suggesting an association of the activated TGF-β1 gene transcription with hepato- carcinogenesis. Patients with HCC were divided into 2 groups according to their TGF-β1 mRNA above （group A, n=28）or below （group B, n=25） the mean level. Statistical results demonstrated that TGF-β1 mRNA expression level was correlated with patients age, serum levels of CEA, CA19-9 and viral copy number （P<0.05）. Conclusion: Although this is a small sample size pilot study these findings imply that quantitative measurement of TGF-β1 mRNA level in peripheral blood may be a complementary serologic marker of HCC.0|30|0Updated：2026-03-12
- Abstract：Objective: To investigate the expression of aquaporin 1 in cervical squamous carcinomas （CSC） and cervical precancerous lesions, and the relationship between the tumor clinicopathological parameters, prognosis and the expression of AQP1. Methods: Immunohistochemical method （EliVision） was used to detect the expression of AQP1 in samples from 106 patients [20 with normal cervical tissue, 30 with cervical intraepithelial neoplasia （stage I and II） and 56 with CSC]. Survival analysis was performed by Kaplan-Meier method. Results: AQP1 protein was expressed in vascular endothelia of all samples. It showed upregulation of AQP1 expression in CSC. There was a significant difference between CSC and normal cervical tissues （P<0.05）. AQP1 was expressed in some tumor cells and unexpressed in normal squamous epithelial cells. And APQ1-expressing tumor cells were positively related to lymph node metastasis. Patients with APQ1-expressing tumor cells had the lower survival rate than the ones without. Conclusion: Abnormal expression of AQP1 plays an important role in the development of CSC. Positive expression of AQP1 in tumor cells maybe enhances tumor metastasis and could be used as a marker for tumor prognosis.0|49|3Updated：2026-03-12
- Abstract：Objective: To explore the regulating effects of cefodizime on cytokines expression of neutrophil response to Klebsiella pneumoniae （Kle. p） treatment. Methods: We detected the types and expression of cytokines secreted by neutrophils by cDNA array and RT-PCR. We also analyzed the changes of signal transduction in this process by detecting the expression of toll like receptor 4 （TLR4） and the inhibitor factor of κBα （I-κBα） expressed by neutrophils. The activity of NF-κB DNA-binding in neutrophils was measured by electrophoretic mobility shift assay （EMSA）. Results: Cefodizime increased the neutrophils production of TNF-α, IL-1β and the mRNA expression of TLR4 in the early stage of Kle. p stimulation in mice, which seemed corresponding to the enhanced NF-κB DNA-binding activity. Conclusion: Cefodizime regulates the cytokines expression of neutrophils through the LPS-TLR4-NF-κB pathway by affecting the expression of TLR4 mRNA and the DNA binding activities of NF-κB in mice with the challenge of Kle. p.0|16|0Updated：2026-03-12
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