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Volume ， Issue 3 ， 2008
- Abstract：Objective:To investigate the effects of rosiglitazone,a synthetic ligand of peroxisome proliferators-activated receptor gamma（PPARγ）,on the expression of acyl-coenzyme A:cholesterol acyltransferase-1（ACAT-1） in phorbol myristate acetate（PMA）-pretreated THP-1 cells after the inducement of advanced glycation end products（AGEs）.Methods:After THP-1 cells were cultured in the presence of 0.1 μmol/L PMA for 72 h to induce phagocytic differentiation,the obtained THP-1 macrophages were treated with rosiglitazone for 4 h at different concentrations（1,5 or 10 μmol/L） and then exposed to AGEs-modified bovine serum albumin（AGEs-BSA） for 24 h at a concentration of 200 mg/L.Reverse transcription polymerase chain reaction（RT-PCR） and Western blot analysis were performed to detect the mRNA and protein expressions of ACAT-1 respectively.Results:Administration of AGEs-BSA（200 mg/L） into the THP-1 macrophages resulted in up-regulation of ACAT-1 at mRNA and protein levels when compared with the expressions in macrophages incubated with serum-free RPMI1640.Pretreatment of rosiglitazone inhibited significantly the increased expression of ACAT-1 induced by AGEs-BSA in a concentration-dependent manner.Conclusion:PPARγ activation by rosiglitazone down-regulates ACAT-1 expression induced by AGEs in THP-1 macrophages,which might provide a new way for treating atherogenesis in diabetic patients.0|19|0Updated：2026-03-12
- Abstract：Hydroxylammonium nitrate（HAN） is a major constituent in a class of liquid monopropellants and is extensively used in nuclear industry and space propulsion.Previous toxicological studies have focused on oral,inhalation and dermal routes of exposure to HAN-based propellant blends.In this study,acute and subchronic toxicity of HAN in Wistar rats by intraperitoneal injections were evaluated.In this acute study,doses of HAN at 115,125,135,147,160 or 174 mg/kg were administered.No adverse effects were observed during a 14-day period and at gross histopathological examination.In the subchronic study,HAN at 7,14 or 28 mg/kg were administered for 13 weeks.The treatment with HAN caused significant changes in the weight of spleen,in the level of hematological parameters,total bilirubin,direct bilirubin,uric acid and carbondioxidecombining power and histopathological damages of the lung,liver,spleen and kidney.Overall,the study suggests that 13-week HAN treatment caused abnormal hematological changes and tissue lesions,and the risk of toxicity to mammals is not negligible.0|13|0Updated：2026-03-12
- Abstract：Objective:To express the soluble recombinant hemangiopoietin protein in E.coli BL21（DE3）.Methods:Using human fetal live cDNA as a template,a partial cDNA fragment of HAPO coding N-terminal region was subcloned into plasmids pTrc99,pQE60 and pET32c to construct different recombinant prokaryotic expression systems.After selecting,the soluble rhHAPO fusion protein was expressed stably in E.coli BL21（DE3） by vector pET32c-HAPO and further isolated by nickelnitrilotriacetic acid（NTA） affinity chromatography.After cleavage with enterokinase,the rhHAPO protein was applied to Fast Flow SP sepharose column.Results:The rhHAPO protein had a purity of more than 95% and a good bioactivity based on the cell adhesion assay in ECV304 cells.Conclusion:We have established a protein engineering system to produce rhHAPO which may provide the possibility for clinical application.0|16|0Updated：2026-03-12
- Abstract：Objective:To determine the effect of antiparallel phosphorothioate triplex-forming oligonucleotide（apsTFO）,which was designed according to shear stress response element（SSRE） in tissue factor（TF） gene promoter region,on the expression of endothelial TF in carotid artery stenosis rats.Methods:Rat model of severe carotid artery stenosis were inflicted by silica gel tube ligation.Half an hour before the model infliction,GT20-apsTFO,GT20-psTFO and GT21-apsTFO labeled with green fluorescence（FITC） were injected into the vena caudalis of rat at a dose of 0.5 mg/kg.Half an hour,4 or 9 h after the ligation,the distribution of TFO in the common carotid artery,the liver and the kidney was detected with aid of fluorescence microscopy.And the mRNA and protein expressions of TF,Egr-1 and Sp1 in the above-mentioned organs were determined with in situ hybridization and immunohistochemical assay respectively in 6 h after the model establishment,and the results were analyzed with an image analysis system.Results:Only in 1 h after TFO injection,fluorescent granules appeared in the liver,the kidney and the vascular wall and lumen of carotid artery,and then in 4.5 h,they still deposited in above sites except the vascular lumen.GT20-apsTFO and GT21-apsTFO significant down-regulated the mRNA and protein expressions of TF compared to the rats without treatment（P<0.05）,and the former apsTFO had a more stronger effect than the later（P<0.05）.GT20-psTFO had no such effect（P>0.05）.The 3 TFOs had no inhibition on the mRNA and protein expressions of Egr-1 and Sp1.Conclusion:Pretreated apsTFO can partly come into the vascular endothelial cells,and inhibit TF expression induced by shear stress,but had no effect on Egr-1 and Sp1 gene expressions.0|15|0Updated：2026-03-12
- Abstract：Objective:To observe the expressions of claudin-4 and claudin-1 in endometrial cancer and explore their correlations with clinicopathological parameters of endometrial cancer.Methods:Immunohistochemical methods（SP） were used to detect the expressions of claudin-4 and claudin-1 in 52 tissue samples of endometrial cancer,24 of atypical hyperplasia,20 of pericancerous endometrium,and 19 of endometrium at proliferative phase.And then the expressions were analyzed statistically to find out the correlations with clinicopathological parameters of endometrial cancer.Results:Positive rate of claudin-4 was 36.8%,70.8% and 90.4% in endometrium at proliferative phase,atypical hyperplasia and endometrial cancer,respectively,with significantly differences between them（P<0.05）,and it was statistically different between pericancer endometrium and endometrial cancer（P<0.05）.Positive rate of claudin-1 was 89.5%,66.7% and 63.5%,respectively showing a descending tendency and significantly differences between endometrium at proliferative phase and endometrial caner（P<0.05）,and it was also statistically significantly different between pericancer endometrium and endometrial cancer（P<0.05）.The high expression rate of claudin-4 was related to invasion depth,but not to histological grading,pathological staging or lymph node metastasis of endometrial cancer,and the low expression of claudin-1 in endometrial cancer was not associated with histological grading,pathological staging,invasion depth or lymph node metastasis.Conclusion:The expression levels of claudin-4 and claudin-1 are correlated with onset and development of endometrial cancer.0|78|5Updated：2026-03-12
- Abstract：Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese medicine.Methods:HacaT cell of keratinocytes lines were cultured and treated with the polysaccharides.The total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse thanscription.The obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA chip.The images were scanned and analyzed with special software.The scan data were analyzed with software and checked by real time PCR.Results:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones down-regulated.It was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin aging.Two ways are anastomotic.Conclusion:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin aging.It shows that the results of gene array reliable by real time PCR.0|61|14Updated：2026-03-12
- Abstract：Objective:To improve the recognition and diagnosis on the bronchopulmonary infection with Lophomonas blattarum（L.blattarum）.Methods:The clinical characteristics of 2 patients diagnosed and treated in our hospital were reported,and 42 cases that had been reported from years 1993 to 2007 are analyzed.Results:In our report,the first patient attacked serious asthma time after time,the second patient suffered from bronchiectasis with a protracted infection course.Forty-four cases all have pathogen examination and parasitic expertise.The most common symptoms are fever,cough and expectoration.1/3 of the patients have increased acidophilic granulocyte in peripheral blood.Chest X film and CT scanning suggest changes were similar to pneumonia.Chronic cases are manifested with bronchial asthma,bronchiectasis and pulmonary abscess.L.blattarum found in phlegm or specimen collected by bronchoscopy provides the most reliable evidence for the diagnosis of this disease.Conclusion:Bronchopulmonary L.blattarum infection is a new kind of diseases.The clinical manifestations are similar to pneumonia,asthma,bronchiectasis infection or pulmonary abscess.L.blattarum found in sputum smear,bronchoscopic brush smear,bronchoscopic biopsy smear,or bronchoalceolar lavage under microscope is the foundation of the diagnosis.The pathogen species has not been finally confirmed.It is still unclear how the pathogen exists in the natural environment,how to transmit to persons and what kind of people would suffer from the disease more easily.Treatment only with antibiotics is not effective to this disease.Metronidazole with dosage of 0.5 g per time and twice per day was effective to most patients,the period of treatment need to last 14-38 d,but multidrug resistance case had been reported.0|19|1Updated：2026-03-12
- Abstract：GABAergic neurons are the major inhibitory interneurons that powerfully control the function of spinal neuronal networks.In recent years,tremendous progresses have been made in understanding the transcriptional control of GABAergic neuron development in the dorsal spinal cord.New experimental approaches provide a relatively high throughput way to study the molecular regulation of subgroup fate determination.Our understanding of the molecular mechanisms on GABAergic neuron development in the dorsal spinal cord is rapidly expanding.Recent studies have defined several transcription factors that play essential roles in GABAergic neuron development in the spinal dorsal horn.Here,we review results of very recent analyses of the mechanisms that specify the GABAergic neuron development in the dorsal spinal cord,especially the progresses in the homeodomain（HD） and basic-helix-loop-helix（bHLH） containing transcription factors.0|14|0Updated：2026-03-12
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