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Volume ， Issue 5 ， 2008
- Abstract：Objective: To analyze the differential expression genes （DEGs） between Barrett’s esophagus （BE） and normal esophagus mucosa and explore the target genes related to the development and progression of BE. Methods: The total RNAs of matched BE and normal esophagus mucosa of BE patients were isolated using one step Trizol method. Matched RNAs were qualified using 10 g/L agarose gel electrophoresis. cRNAs were synthesized, fluorescence labeled and purified after total RNAs were purified. The RNAs of BE and normal esophagus mucosa were hybridized with Agilent oligomicroarray （30 968 probes）. The fluorescence intensity features were detected by Agilent scanner and quantified by feature extraction software. Results: （1） The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality; （2） There were 142 up-regulated genes and 284 down-regulated genes among 2-fold DEGs. Conclusion: Microarray-based studies are feasible in endoscopically obtained tissues. Many BE-associated genes are screened by the high-throughput gene chip. The development and progression of BE is a complicated process involving multiple genes and multiple procedures, and functional study of these genes may help to identify the key genes or pathways involved in the pathogenesis and development of BE.0|15|0Updated：2026-03-12
- Abstract：Objective: To investigate the characteristics of fat metabolism in rat skeletal muscle after hypobaric hypoxia acclimation. Methods: Sprague-Dawley rats were divided into 3 groups randomly: control group （H0）, hypoxic 5-day group （H5）, and hypoxic 15-day group （H15）. Animals of H5 and 15 groups were exposed to hypobaric hypoxia chamber simulating 5 000 m high altitude for 5 d or 15 d respectively, 23 h per day. H0 group stayed outside of chamber. The level of fatty acid oxidation and uptake, and glucose oxidation were examined, and the level of non-esterified fatty acids （NEFA）, ATP and phosphocreatine （PCr） were also assayed in rat skeletal muscles. Results: The contents of ATP and PCr in H5 group were lower than those in H0 and H15 groups （P<0.05）, while there was no significant difference between H0 and H15. Compared with H0, the blood NEFA level in all hypoxia groups was increased significantly （P<0.05）. The muscle NEFA level in H15 group was greatly higher than that in H0 and H5 groups. The rates of fatty acid oxidation and uptake in H15 group were significantly higher than those in H0 and H5 groups （P<0.05）, and the rate of glucose oxidation in all hypoxia groups was significantly decreased than that in H0 group （P<0.05）. Conclusion: It is concluded that the enhanced fat oxidation may be one of the mechanisms in the maintenance of energy homeostasis after hypobaric hypoxic acclimation.0|22|4Updated：2026-03-12
- Abstract：Objective: To explore the effect of cellular immunity in halothane hepatitis. Methods: Hepatotoxicity model was established by exposing male Hartley guinea pigs to 1% halothane via inspiration for 4 h each time for 1 or 3 times within a 42-day interval. Then their hepatocytes and lymphocytes were collected and divided into 2 parts for different cultures. Hepatocytes were cultivated with or without 1% halothane for 4 h and lymphocytes were cultivated with or without 12.5 μg/ml trifluoroacetylated guinea pig serum albumin （TFA-GSA）. Then the 2 kinds of hepatocytes were co-cultivated with lymphocytes （1:100） with or without TFA-GSA induction respectively and the supernatant fluid was taken after 24, 48 and 72 h to determine the concentration of alanine aminotransferase （ALT）. The halothane cultivated hepatocytes were co-cultivated with various proportion of TFA-GSA antigen induced lymphocytes and ALT was determined after 48 h to determine the proper proportion of hepatocytes and lymphocyte. Results: Lymphocytes of 3 times halothane induced guinea pigs caused a significant increase of ALT in hepatocytes with or without halothane induction. But the lymphocytes of 1 time halothane induced guinea pigs only caused a significant increase of ALT in hepatocytes with induction of halothane. The increase of ALT was only seen after 48- and 72-hour co-culture. The proper proportion of hepatocytes and lymphocytes was 1:100 for lymphocytes cytotoxicity. Conclusion: Lymphocytes is sensitized after inhalation of halothane and generates cytotoxicity to hepatocytes. The immune response of lymphocytes to hepatocytes will be enhanced by repeated inhalation of halothane. The cellular immunity may be one of the mechanisms of halothane induced hepatotoxicity.0|9|0Updated：2026-03-12
- Abstract：Objective: To investigate the changes of autonomic nervous system （ANS） function during the initial phase at acute high-altitude exposure. Methods: Ninety-nine healthy sea-level male residents were studied in Chengdu plain and then Tibet plateau. Heart rate variability （HRV）, cold pressor test （CPT）, resting heart rate （HR） and blood pressure （BP） were measured at baseline （560 m altitude） and in 2 to 4 d after arriving at Tibet plateau （3 675 m altitude） to assess the ANS function. Results: Compared with baseline, on day 2 in Tibet the standard deviation of normal to normal intervals （SDNN）, high-frequency （HF） power, total power （TP）, root mean square of delta RR （rMSSD）, percentage of delta RR>50 ms （PNN50）, normalized high-frequency （HFnu） and fractal dimension （FD） decreased significantly （SDNN, HF, TP P<0.01, rMSSD, PNN50, HFnu, FD P<0.05）, while the normalized low-frequency （LFnu） and LF/HF increased significantly （P<0.01）. During day 3–4 in Tibet, SDNN, rMSSD, HF, TP and HFnu tended to rebound while LFnu and LF/HF decreased towards baseline day by day. In addition, in Tibet the increase in systolic pressure （SP） and diastolic pressure （DP） during CPT decreased significantly （P<0.01, 0.05）, but resting HR increased compared with baseline （P<0.01）. Conclusion: ANS modulation is generally blunted, and the relatively predominant sympathetic control is enhanced originally, then it reverts to the sea level states gradually during the initial days of acute high-altitude exposure.0|11|1Updated：2026-03-12
- Abstract：Objective: To elucidate the role of the autonomic nervous system （ANS） in acute mountain sickness （AMS） during the initial phase at acute high-altitude exposure. Methods: Ninety-nine healthy sea-level residents rapidly ascended to Tibet plateau （3 675 m altitude） by airplane from Chengdu plain （560 m altitude）. ANS function was tested in plain and day 2–4 in Tibet by heart rate variability （HRV）, cold pressor test （CPT）. AMS was evaluated by clinic symptomatic scores. All subjects were divided into non-AMS group （57, scores≤4） and AMS group （42, scores>4）. Results: Compared with non-AMS group, AMS group had higher standard deviation of normal to normal intervals （SDNN）, root mean square of delta RR （rMSSD）, low-frequency （LF） power, and normalized low-frequency （LFnu） power in plain （P<0.05）. After arrival at 3 675 m altitude, AMS group had greater reduction in percentage of delta RR>50 ms（PNN50）, rMSSD （P<0.01） and SDNN, LF, total power （TP） （P<0.05）. Although no significant differences in the increase of SP and DP during CPT were found between 2 groups in plain, the SP increase during CPT of AMS group was less than non-AMS group （P<0.05） at 3 675 m altitude. AMS symptomatic scores was not only positively correlated with SDNN, rMSSD, LF/HF in plain （P<0.05）, but also negatively correlated with HFnu in plain （P<0.05）. Conclusion: During the initial high altitude exposure, ANS modulation is generally blunted, but the relatively predominant sympathetic control is enhanced, and this characteristic change of ANS function is positively correlated with the development of AMS.0|13|3Updated：2026-03-12
- Abstract：Objective: To investigate the effect of behavior training on the learning and memory of young rats with fetal growth restriction （FGR）. Methods: The model of FGR was established by passive smoking method to pregnant rats. The new-born rats were divided into FGR group and normal group, and then randomly subdivided into trained and untrained group respectively. Morris water maze behavior training was performed on postnatal months 2 and 4, then learning and memory abilities of young rats were measured by dark-avoidance testing and step-down testing. Results: In the dark-avoidance and step-down testing, the young rats’ performance of FGR group was worse than that of control group, and the trained group was better than the untrained group significantly. Conclusion: FGR young rats have descended learning and memory abilities. Behavior training could improve the young rats’ learning and memory abilities, especially for the FGR young rats.0|17|0Updated：2026-03-12
- Abstract：Objective: The aim of this preliminary qualitative study was to gain insight into community health needs in order to develop health program for community in Chongqing. Methods: Totally 40 participants were assigned into 6 focus group discussions. All groups were led by local language speakers, and their talking was recorded after gaining informed consent. Transcribed data were coded and subjected to thematic analysis. Results: The main obtained themes were that community health problems were chronic noncommunicable disease, main health service needs included regularly free check-up and health education, and perceived health risk factors were unhealthy lifestyle and behavior as well as environment problems. Conclusion: Our community health needs assessment （CHNA） with FGDs indicate that residents realize the importance of prevention of disease. Our study identifies that primarily community health promotion is one of the priorities of community health service needs, including reorienting health service, health education, guiding behavior or lifestyle, and creating healthy environments. The findings of this study can provide guidance to the development of more effective and pertinent health program in this community.0|88|1Updated：2026-03-12
- Abstract：Objective: To quantitatively identify and grade trigeminal sensory functions after 3 major surgical procedures of trigeminal neuralgia using a newly developed quantitative sensory testing technique, current perception threshold measurement （CPTM）. Methods: In the current study, there were 48 trigeminal neuralgia patients without history of prior surgical treatment. These patients received one of the following 3 surgical procedures, microvascular decompression （MVD）, peripheral nerve block with alcohol （PNB）, or percutaneous radiofrequency thermocoagulation （PRFT）. The quantitative sensory testing measurement, CPTM, and conventional qualitative sensory testing measurements were performed preoperatively and postoperatively to evaluate and grade the trigeminal sensory functions. All 3 major cutaneous sensory fiber types, large myelinated fibers （A beta）, small myelinated fibers （A delta） and unmyelinated fibers（C） were allowed to quantitatively evaluate and grade by CPTM. The results of the measurements were statistically analyzed using a one-way analysis of variance （single factor）. Each subject was his/her own control for comparison of the preoperative to postoperative state on the asymptomatic and symptomatic sides. Subjects were tested 48 h preoperatively and 4 weeks postoperatively. Results: PNB with alcohol and PRFT caused significant sensory dysfunction postoperatively in every fiber type, indicating damage to all fibers. On the contrary, the sensory function in all 3 fiber types was unchanged after MVD management. Conclusion: Among the 3 major surgical procedures tested, only MVD preserves sensory function in trigeminal system. CPTM is of quantitative nature on the evaluation of sensory functions of nerve fibers0|22|2Updated：2026-03-12
- Abstract：To differentiate renal oncocytoma from renal carcinoma, the clinical data of four patients with incidentally found renal oncocytomas were studied in this report. And additional Immunohistochemistry examinations were done to confirm diagnosis. Renal oncocytomas were found incidentally in four patients during medical examination. No characteristic changes were found in laboratory tests. Radiology examination provided the location and possibility of renal oncocytoma. History, laboratory test and radiology exanimation indicated the diagnosis of renal oncocytoma, but the final identify of renal oncocytoma need pathology examination.0|16|0Updated：2026-03-12
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