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Volume 27 ， Issue 5 ， 2012
- Abstract：Objective: Diabetic nephropathy （DN） is one of the most common causes of end-stage renal failure. The pathogenesis of progressive renal injury is multifactorial and the mechanism by which hyperglycemia causes microangiopathy is still poorly understood. The WNT pathway is activated in DN and regulating β-catenin protein levels is referred to as the canonical Wnt/β-catenin pathway. Because the renin angiotensin system has been reported to be an important contributory factor in the pathophysiology of DN, exogenous administration of angiotensin II receptor antagonist may be beneficial in counteracting some biochemical or functional changes of DN. The aim of the study was to determine the β-catenin expression and the possible protective effects of irbesartan, an angiotensin II type 1 receptor blocker （ARB） in a rat model of streptozotocin（STZ）-induced diabetic nephropathy. Methods: STZ-induced DN in rats was assessed biochemically by measuring urine volume, protein and creatinine clearance as well as Kidney weight/body weight （KW/BW） and the index of mesangial expansion. Three groups of male Sprague-dawley rats were used. The ?rst group consisted of non-diabetic control rats （control）. The second group was the untreated diabetic rats（STZ+vehicle）. The third group consisted of diabeti rats treated with irbesartan, 50 mg/kg for 12 weeks （STZ+irbesartan）. Immunohistochemical stainings and real time PCR for β-catenin were performed in renal cortex of rat modals. Results: Marked hyperglycemia, polyuria, proteinuria, renal hypertrophy, mesangial matrix expansion and glomerular hyper?ltration were observed in STZ diabetic rats. The levels of microalbuminuria and KW/BW in the STZ+irbesartan group were lower than those in the STZ+vehicle group （P<0.05）. The up-regulated immunostaining and mRNA expression of β-catenin were decreased in renal cortic of the Irbesartan-treated diabetic group, but there was no significant difference compared to the untreated diabetic group. Conclusion: The data suggest that irbesartan ameliorates proteinuria and renal hypertrophy, charactered damages of STZ-induced early-stage DN in rats, but its effective drug target is not to inhibit the up-regulated expressions of β-catenin.0|35|0Updated：2026-03-12
- Abstract：Objective: To estimate the effect of statins use on the prevention of venous thromboembolism （VTE）. Methods: We systematically searched MEDLINE （1980-June 2012）, EMBASE （1980-June 2012）, Google Scholar, Cochrane Library, and ISI Web of Science, manually reviewed references, and contacted experts. Case-control studies and cohort studies that compared any dose of statin with no statin or placebo are included. Data extraction and study quality evaluation were independently conducted in duplicate. Results: 12 studies including four cohort studies and eight case-control studies were identified and eligible for meta-analysis. Upon meta-analysis, statin use was associated with a statistically significant reduction in the odds of developing VTE （OR 0.91, 95% CI 0.86-0.96）. Conclusion: This meta-analysis of current and available literature suggests that statins can reduce patient’s risk of developing VTE. Due to the limitations of observational study, this conclusion should be considered with caution, and additionally, specifical well-designed trials are needed.0|27|1Updated：2026-03-12
- Abstract：Objective: To figure out the effect of somatostatin analogue Octreotide on proliferation and invasion of human hepatocellular carcinoma cell MHCC97-H and the underlying mechanism in vitro and in vivo. Methods: MHCC97-H cells were treated with Octreotide at the concentration of 0.2 ug/mL in vitro, proliferation related to time was evaluated. After treated with Octreotide at the concentration of 0.2 ug/mL for 48 h, MHCC97-H cells were observed by transmission electron microscope. Cell proliferation was detected by MTT assay after MHCC97-H cells were treated with Octreotide at different concentrations including 0.05, 0.1, 0.2, 0.4, 0.6 and 0.8 ug/mL for 36 h in vitro. 27 nude mice, in which MHCC97-H tumor mass was planted orthotopically, were divided into 3 groups randomly including control group （intraperitoneal injection with equal volume normal saline; n=8）, low dose treated group （intraperitoneal injection with Octreotide at 50 ug/kg?d; n=9） and large dose treated group （intraperitoneal injection with Octreotide at 200 ug/kg?d; n=10）. All mice were raised for 35 d and sacrificed. The information about survival time, the weight at death point and the pathology change of liver and lung was collected. The expression of vascular endothelial growth factor （VEGF） and matrix metalloproteinases-2 （MMP-2） in mouse HCC tissues were detected by immunohistochemistry finally. Results: MTT assays showed that Octreotide inhibited the proliferation of MHCC97-H cells significantly. Apoptosis cells were found by transmission electron microscope after treatment with Octreotide at 0.2 ug/mL for 48 h in vitro. The proliferation was inhibited significantly by Octreotide in a dose-dependant manner （r=0.86, P<0.01）. Compared with control group, the treated group had the heavier weight at death point and lower intrahepatic metastasis ratio （P<0.05）, meanwhile, there was not significant difference in treated groups （P>0.05）. The positive expression ratios of VEGF and MMP-2 in treated groups were lower than those in control group （P<0.05）, while there was no apparent difference in treated groups （P>0.05）. Conclusion: Octreotide could inhibit the proliferation of MHCC97-H cells in vitro via inducing apoptosis and the inhibitory function acts in a dose-dependant manner. Octreotide could improve survival of mice with MHCC97-H cells and inhibit the metastasis of MHCC97-H cells in vivo. Regulation of VEGF and MMP-2 expression by Octreotide would be involved in its inhibition in vivo.0|22|0Updated：2026-03-12
- Abstract：Objective: To retrospectively analyze the clinical data of 275 cases of cervical cancer （CC） in our hospital, and investigate the clinicopathological parameters of cervical cancer based on the expression of p16INK4A proteins. Methods: The clinical information of 275 patients with cervical cancer were retrospectively analyzed between 2006 and 2011, including the patients’ age, clinical FIGO stage, differentiation, histologic grade, infiltration depth, treatment, pathological diagnosis after surgery, and results of following-up.Immunohistochemistry was also done on sections of confirmed cancer specimens without prior chemotherapy/radiotherapy. Results: Among various clinicopathological parameters, the median age was 48 years old. The grade was significantly associated with histological type, HPV infection and with lymph node invasion. FIGO stage was strongly correlated to the infiltration depth and lymph node metastasis. P16INK4A expression was significantly correlated with histologic grade. However, there were no differences between p16INK4A staining and patient’s age, histopathology and lymph node metastases. Conclusion: The incidence of cervical cancer becomes increasingly younger. Additionally, p16INK4A can function as a diagnostic marker of cervical carcinomas.0|23|1Updated：2026-03-12
- Abstract：Objective: To determine whether CA-125 is elevated in medically stable patients with chronic atrial fibrillation （AF） compared with patients without AF and to examine whether levels of CA-125 are associated with demographic and clinical variables in a sample of patients under study. Methods: 55 patients with chronic AF and 58 control patients in sinus rhythm were included into the study. Patients with acute heart failure （HF）, chronic inflammatory or neoplastic disease were excluded from the study. Circulating levels of CA-125 were assessed; all patients underwent clinical examination, assessment and medical records including demographic data, history of comorbid conditions, current use of cardiac medications, and the results of cardiac tests including electrocardiograms. Results: The mean age of the study sample was 53.2±6.5 years and 48% were men. Patients with sinus rhythm were significantly more likely to have lower heart rates, smaller dimensions of left atrium, and to be treated with aspirin. Coumadin, coumadin and digoxin were more often prescribed in patients with chronic AF. The CA-125 levels were significantly higher in patients with chronic AF than in patients in sinus rhythm （48.5±7.65 U/ml and 28.43±5.48 U/ml, P<0.005）. An inverse relation was found between CA-125 levels and left ventricular ejection fraction （LVEF） （r=-0.48, P<0.001）. CA-125 was significantly related to the left atrium （LA） diameter, left ventricular end-diastolic diameter （LVEDD）, left ventricular end-systolic diameter （LVESD） and brain natriuretic peptide （BNP）. There was no significant correlation between CA-125 and age. Conclusion: In subjects with chronic AF, CA-125 levels are increased; CA-125 was significantly related to the LA diameter, LVEDD, LVESD and BNP.0|17|0Updated：2026-03-12
- Abstract：Objective: To investigate the insomnia incidence and treatment status of military retired cadres and offer an instruction for their health care. Methods: We designed a questionnaires composed of general information, health-related factors, diseases, etc. Insomnia manifestations included regular sleep time, shorten sleep time and extent, time of falling asleep, sleep quality, difficultly returning to sleep after awakening at mid-night, insomnia time, etc. Results: A total of 4759 questionnaires （94.2%） were reclaimed. Dyssomnia was found in 1843 persons （38.7%）, including 1006 cases of transient insomnia （44.0%） and 837 cases of acute insomnia （36.6%）. Conclusion: About one third of the examined military retired cadres have sleep problems, so they should enhance the health care consciousness and receive proper nurse and treatment.0|20|0Updated：2026-03-12
- Abstract：As the population ages, older people’s health and quality of life are becoming a matter of public concern increasingly. Through review of the literatures and analysis of the reasons for falls in older people, we worked out some measures to cope with such a situation, provided the self-care knowledge of falls prevention for older people and improved the quality life of older persons. The recovery process is long, if the old people fall down, which brings a lot of inconvenience to individuals and families, and it can also cause a lot of complications. If wary of fall risk factors, the quality of life of older persons can be improved. According to the different conditions of the elderly, we should take effective measures to create suitable living environment for senior citizens, and propagandize the knowledge of the old people’s health care, which has the great significance in preventing the elderly’s fall, and improving their living quality.0|46|1Updated：2026-03-12
- Abstract：<正>1. Background Hepatocellular carcinoma （HCC） is one of the most common neoplasms encountered, and its incidence is increasing worldwide because of the increasing prevalence of hepatitis B virus （HBV） and hepatitis C virus （HCV） infection [1, 2].0|5|1Updated：2026-03-12
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