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Amplificação de sinal mediada por nickase CRISPR/Cas9 integrando a atividade de clivagem trans de Cas12a para detecção altamente seletiva e sensível de mutações de base única

  • role： Contribute equally, First author同等贡献者, 第一作者
  • Affiliation：

    Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China

Xiao-Wen Fan1,  
  • role： Contribute equally同等贡献者
  • Affiliation：

    Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China

Zi-Fan Gao1,  
  • role： Contribute equally同等贡献者
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

Dong-Dong Ling2,  
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

De-Hui Wang2,  
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

Ying Cui2,  
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

Hui-Qun Du2,  
  • role： Corresponding author通信作者
  • Affiliation：

    Department of Health Medicine, the Eighth Medical Center of PLA General Hospital, Beijing 100093, China

  • Email：leecl316@163.com
  • Introduction：* leecl316@163.com
Chun-Lin Li3*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

  • Email：dr_zhouxing@126.com
  • Introduction：dr_zhouxing@126.com
Xing Zhou12*

Resumo

Palavras-chave

Cas9 nickase;CRISPR-Cas12a;Polymerase;Single base mutations

References

  1. 1..
    Suzuki M, Takahashi T. Aberrant DNA replication in cancer. Mutat Res. 2013;743–744:111–7.
  2. 2..
    Hu T, Chitnis N, Monos D, Dinh A. Next-generation sequencing technologies: an overview. Hum Immunol. 2021;82(11):801–11.
  3. 3..
    Nakagawa H, Fujita M. Whole genome sequencing analysis for cancer genomics and precision medicine. Cancer Sci. 2018;109(3):513–22.
  4. 4..
    Roy S, Juste SS, Sneider M, Auradkar A, Klanseck C, Li Z, et al. Cas9/nickase-induced allelic conversion by homologous chromosome-templated repair in Drosophila somatic cells. Sci Adv. 2022;8(26):eabo0721.
  5. 5..
    Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, et al. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016;353(6305): aaf8729.
  6. 6..
    Wang Q, Liu J, Janssen JM, Gonçalves MAFV. Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants. Nucleic Acids Res. 2023;51(7):3465–84.

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