이전|다음

CRISPR/Cas9 닉커제 매개 신호 증폭과 Cas12a의 트랜스클리브 활성 통합을 통한 단일 염기 돌연변이의 고선택적이고 민감한 검출

  • role： Contribute equally, First author同等贡献者, 第一作者
  • Affiliation：

    Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China

Xiao-Wen Fan1,  
  • role： Contribute equally同等贡献者
  • Affiliation：

    Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China

Zi-Fan Gao1,  
  • role： Contribute equally同等贡献者
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

Dong-Dong Ling2,  
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

De-Hui Wang2,  
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

Ying Cui2,  
  • Affiliation：

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

Hui-Qun Du2,  
  • role： Corresponding author通信作者
  • Affiliation：

    Department of Health Medicine, the Eighth Medical Center of PLA General Hospital, Beijing 100093, China

  • Email：leecl316@163.com
  • Introduction：* leecl316@163.com
Chun-Lin Li3*,  
  • role： Corresponding author通信作者
  • Affiliation：

    Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China

    Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China

  • Email：dr_zhouxing@126.com
  • Introduction：dr_zhouxing@126.com
Xing Zhou12*

추상적인

키워드

Cas9 nickase;CRISPR-Cas12a;Polymerase;Single base mutations

References

  1. 1..
    Suzuki M, Takahashi T. Aberrant DNA replication in cancer. Mutat Res. 2013;743–744:111–7.
  2. 2..
    Hu T, Chitnis N, Monos D, Dinh A. Next-generation sequencing technologies: an overview. Hum Immunol. 2021;82(11):801–11.
  3. 3..
    Nakagawa H, Fujita M. Whole genome sequencing analysis for cancer genomics and precision medicine. Cancer Sci. 2018;109(3):513–22.
  4. 4..
    Roy S, Juste SS, Sneider M, Auradkar A, Klanseck C, Li Z, et al. Cas9/nickase-induced allelic conversion by homologous chromosome-templated repair in Drosophila somatic cells. Sci Adv. 2022;8(26):eabo0721.
  5. 5..
    Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, et al. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 2016;353(6305): aaf8729.
  6. 6..
    Wang Q, Liu J, Janssen JM, Gonçalves MAFV. Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants. Nucleic Acids Res. 2023;51(7):3465–84.

더 읽기

The above content is generated by Large Model Translation. The translated content is for reference only. We do not assume any commercial or legal responsibilty for any consequences arising from the use of our website