이전|다음

주위세포는 혈관 반응성과 장벽 기능을 유지하여 쥐와 생쥐를 패혈증으로 인한 손상으로부터 보호합니다: miRNA 및 마이크로소포의 역할

  • role： First author第一作者
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Zi-Sen Zhang,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Yi-Yan Liu,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Shuang-Shuang He,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Dai-Qin Bao,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Hong-Chen Wang,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Jie Zhang,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Xiao-Yong Peng,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Jia-Tao Zang,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Yu Zhu,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Yue Wu,  
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

Qing-Hui Li,  
  • role： Corresponding author通信作者
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

  • Email：lt200132@tmmu.edu.cn
  • Introduction：* lt200132@tmmu.edu.cn;
Tao Li*,  
  • role： Corresponding author通信作者
  • Affiliation：

    State Key Laboratory of Trauma, Burns and Combined Injury, Department of Shock and Transfusion, Research Institute of Surgery, Daping Hospital, Army Medical University, Chongqing 400042, China

  • Email：lmliu62@tmmu.edu.cn
  • Introduction：lmliu62@tmmu.edu.cn
Liang-Ming Liu*

추상적인

BackgroundVascular hyporeactivity and leakage are key pathophysiologic features that produce multi-organ damage upon sepsis. We hypothesized that pericytes, a group of pluripotent cells that maintain vascular integrity and tension, are protective against sepsis via regulating vascular reactivity and permeability.MethodsWe conducted a series of in vivo experiments using wild-type (WT), platelet-derived growth factor receptor-β (PDGFR-β)-Cre+mT/mG transgenic mice and Tie2-Cre+Cx43flox/flox mice to examine the relative contribution of pericytes in sepsis, either induced by cecal ligation and puncture (CLP) or lipopolysaccharide (LPS) challenge. In a separate set of experiments with Sprague–Dawley (SD) rats, pericytes were depleted using CP-673451, a selective PDGFR-β inhibitor, at a dosage of 40 mg/(kg·d) for 7 consecutive days. Cultured pericytes, vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs) were used for mechanistic investigations. The effects of pericytes and pericyte-derived microvesicles (PCMVs) and candidate miRNAs on vascular reactivity and barrier function were also examined.ResultsCLP and LPS induced severe injury/loss of pericytes, vascular hyporeactivity and leakage (P<0.05). Transplantation with exogenous pericytes protected vascular reactivity and barrier function via microvessel colonization (P<0.05). Cx43 knockout in either pericytes or VECs reduced pericyte colonization in microvessels (P<0.05). Additionally, PCMVs transferred miR-145 and miR-132 to VSMCs and VECs, respectively, exerting a protective effect on vascular reactivity and barrier function after sepsis (P<0.05). miR-145 primarily improved the contractile response of VSMCs by activating the sphingosine kinase 2 (Sphk2)/sphingosine-1-phosphate receptor (S1PR) 1/phosphorylation of myosin light chain 20 pathway, whereas miR-132 effectively improved the barrier function of VECs by activating the Sphk2/S1PR2/zonula occludens-1 and vascular endothelial-cadherin pathways.ConclusionsPericytes are protective against sepsis through regulating vascular reactivity and barrier function. Possible mechanisms include both direct colonization of microvasculature and secretion of PCMVs.

키워드

Pericyte;Vascular reactivity;Vascular permeability;Cx43;Microvesicle

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