CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation

Yu Liu; Jing-Song Xu; Li Cao; Shuang Yang; Tian-Ming Li; Hai-Qian Huang; Jun-Heng Zhang; Xue Zhao; Qian Liu; Shun Li; Min Li; Hua Wang

doi:10.1186/s40779-025-00632-0

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CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation

RESEARCH | Updated：2026-01-27

- CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation
- “In the field of organ transplantation, a novel CRISPR-based detection platform was developed, establishing a one-pot RPA-CRISPR reaction system and optimizing it for BKV and JCV detection. This platform can simultaneously and rapidly detect BKV and JCV with high sensitivity, providing absolute quantification, which is suitable for viral load detection and conducive to personalized and precise treatment for organ transplant recipients.”
- Military Medical Research Vol. 12, Issue 12, Pages: 1904-1919(2025)
- Affiliations：
  
  1.Department of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
  2.Department of Pathology, the Fifth People’s Hospital of Shanghai, Fudan University, Shanghai 200240, China
  3.Central Laboratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China
  4.Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China
- Author bio：
  
  * Qian Liu qq2005011@163.com
  Shun Li lishun86@126.com
  Min Li rjlimin@shsmu.edu.cn
  Hua Wang renjijywh@shsmu.edu.cn
- Funds：
- DOI：10.1186/s40779-025-00632-0
  CLC：
- Received：08 November 2024，
  
  Accepted：19 July 2025，
  
  Online First：04 August 2025，
  
  Published：2025-12
- Accepted：
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Yu Liu, Jing-Song Xu, Li Cao, et al. CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation[J]. Military Medical Research, 2025, 12(12): 1904-1919.
DOI：

Yu Liu, Jing-Song Xu, Li Cao, et al. CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation[J]. Military Medical Research, 2025, 12(12): 1904-1919. DOI： 10.1186/s40779-025-00632-0.

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CRISPR-based assays for the detection of BK virus and JC virus infections post-kidney transplantation

DOI：10.1186/s40779-025-00632-0