Applications of CRISPR-Cas9 mediated genome engineering

Xiao Yang

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Applications of CRISPR-Cas9 mediated genome engineering

PERSPECRTIVE | Updated：2026-03-12

- Applications of CRISPR-Cas9 mediated genome engineering
- Military Medical Research Vol. 2, Issue 1, Pages: 11-17(2015)
- Affiliations：
  
  State Key Laboratory of Proteomics, Collaborative Innovation Center for Cardiovascular Disorders, Genetic Laboratory of Development and Disease, Institute of Biotechnology, Beijing 100071, China
- Author bio：
  
  *: yangx@bmi.ac.cn
- Funds：
- DOI：
  CLC：
- Published：2015-03
- Accepted：
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Xiao Yang. Applications of CRISPR-Cas9 mediated genome engineering[J]. Military Medical Research, 2015, 2(1): 11-17.
DOI：

Xiao Yang. Applications of CRISPR-Cas9 mediated genome engineering[J]. Military Medical Research, 2015, 2(1): 11-17. DOI：

摘要

Abstract

Targeted mutagenesis based on homologous recombination has been a powerful tool for understanding the mechanisms underlying development

normal physiology

and disease. A recent breakthrough in genome engineering technology based on the class of RNA-guided endonucleases

such as clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9

is further revolutionizing biology and medical studies. The simplicity of the CRISPR-Cas9 system has enabled its widespread applications in generating germline animal models

somatic genome engineering

and functional genomic screening and in treating genetic and infectious diseases. This technology will likely be used in all fields of biomedicine

ranging from basic research to human gene therapy.

关键词

Keywords

references

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