CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

Xiao-Wen Fan; Zi-Fan Gao; Dong-Dong Ling; De-Hui Wang; Ying Cui; Hui-Qun Du; Chun-Lin Li; Xing Zhou

doi:10.1186/s40779-024-00530-x

Your Location：

Home >

Browse articles >

CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

LETTER TO THE EDITOR | Updated：2025-12-13

- CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations
- CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations
- “在xxx领域，xx专家取得了重大进展。他们建立了xx体系/探索了xx主题/验证了xx猜想，为解决xx问题提供了解决方案，为xx的未来研究铺平了道路。”
- MMR 2025年12卷第5期页码：803-806
- Affiliations：
  
  1.Jinling Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210002, China
  2.Department of Orthopedics, Affiliated Hospital of Medical School, Jinling Hospital, Nanjing University, Nanjing 210002, China
  3.Department of Health Medicine, the Eighth Medical Center of PLA General Hospital, Beijing 100093, China
- Author bio：
  
  * leecl316@163.com
  dr_zhouxing@126.com
- Funds：
- DOI：10.1186/s40779-024-00530-x
  中图分类号：
- 纸质出版：2025-05
- Accepted：
Scan QR Code
CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations[J]. MMR, 2025,12(5):803-806.

Cite this article as: Fan XW, Gao ZF, Ling DD, Wang DH, Cui Y, Du HQ, et al. CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations. Mil Med Res. 2024;11(1):25.
CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations[J]. MMR, 2025,12(5):803-806. DOI： 10.1186/s40779-024-00530-x.

Cite this article as: Fan XW, Gao ZF, Ling DD, Wang DH, Cui Y, Du HQ, et al. CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations. Mil Med Res. 2024;11(1):25. DOI： 10.1186/s40779-024-00530-x.

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据

CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

CRISPR/Cas9 nickase mediated signal amplification integrating with the trans-cleavage activity of Cas12a for highly selective and sensitive detection of single base mutations

DOI：10.1186/s40779-024-00530-x