Elucidating regulatory processes of intense physical activity by multi-omics analysis

Ernesto S. Nakayasu; Marina A. Gritsenko; Young-Mo Kim; Jennifer E. Kyle; Kelly G. Stratton; Carrie D. Nicora; Nathalie Munoz; Kathleen M. Navarro; Daniel Claborne; Yuqian Gao; Karl K. Weitz; Vanessa L. Paurus; Kent J. Bloodsworth; Kelsey A. Allen; Lisa M. Bramer; Fernando Montes; Kathleen A. Clark; Grant Tietje; Justin Teeguarden; Kristin E. Burnum-Johnson

doi:10.1186/s40779-023-00477-5

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Elucidating regulatory processes of intense physical activity by multi-omics analysis

RESEARCH | Updated：2025-12-13

- Elucidating regulatory processes of intense physical activity by multi-omics analysis
- Elucidating regulatory processes of intense physical activity by multi-omics analysis
- MMR 2024年11卷第4期页码：479-499
- Affiliations：
  
  1.Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
  2.Environmental and Molecular Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
  3.Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Western States Division, Denver, CO 80204, USA
  4.Computational Analytics Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA
  5.National Security Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA
  6.Los Angeles County Fire Department, Los Angeles, CA 90063, USA
  7.Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Respiratory Health Division, Morgantown, WV 26505, USA
  8.Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR 97331, USA
- Author bio：
  
  *Ernesto.Nakayasu@pnnl.gov;
  jt@pnnl.gov;
  Kristin.Burnum-Johnson@pnnl.gov
- Funds：
  
  the BRAVE Agile Investment from the PNNL
- DOI：10.1186/s40779-023-00477-5
  中图分类号：
- 纸质出版：2024-08
- Accepted：
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Elucidating regulatory processes of intense physical activity by multi-omics analysis[J]. MMR, 2024,11(4):479-499.

Cite this article as: Nakayasu ES, Gritsenko MA, Kim YM, Kyle JE, Stratton KG, Nicora CD, et al. Elucidating regulatory processes of intense physical activity by multi-omics analysis. Mil Med Res. 2023;10(1):48.
Elucidating regulatory processes of intense physical activity by multi-omics analysis[J]. MMR, 2024,11(4):479-499. DOI： 10.1186/s40779-023-00477-5.

Cite this article as: Nakayasu ES, Gritsenko MA, Kim YM, Kyle JE, Stratton KG, Nicora CD, et al. Elucidating regulatory processes of intense physical activity by multi-omics analysis. Mil Med Res. 2023;10(1):48. DOI： 10.1186/s40779-023-00477-5.

摘要

Abstract

Background:

Physiological and biochemical processes across tissues of the body are regulated in response to the high demands of intense physical activity in several occupations

such as firefighting

law enforcement

military

and sports. A better understanding of such processes can ultimately help improve human performance and prevent illnesses in the work environment.

Methods:

To study regulatory processes in intense physical activity simulating real-life conditions

we performed a multi-omics analysis of 3 biofluids (blood plasma

urine

and saliva) collected from 11 wildland firefighters before and after a 45 min

intense exercise regimen. Omics profiles post-

vs.

pre-exercise were compared by Student’s

-test followed by pathway analysis and comparison between the different omics modalities.

Results:

Our multi-omics analysis identified and quantified 3835 proteins

730 lipids and 182 metabolites combining the 3 different types of samples. The blood plasma analysis revealed signatures of tissue damage and acute repair response accompanied by enhanced carbon metabolism to meet energy demands. The urine analysis showed a strong

concomitant regulation of 6 out of 8 identified proteins from the renin-angiotensin system supporting increased excretion of catabolites

reabsorption of nutrients and maintenance of fluid balance. In saliva

we observed a decrease in 3 pro-inflammatory cytokines and an increase in 8 antimicrobial peptides. A systematic literature review identified 6 papers that support an altered susceptibility to respiratory infection.

Conclusions:

This study shows simultaneous regulatory signatures in biofluids indicative of homeostatic maintenance during intense physical activity with possible effects on increased infection susceptibility

suggesting that caution against respiratory diseases could benefit workers on highly physical demanding jobs.

关键词

Keywords

references

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