Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up

Guang-Ying Cui; Ben-Chen Rao; Zhao-Hai Zeng; Xue-Mei Wang; Tong Ren; Hai-Yu Wang; Hong Luo; Hong-Yan Ren; Chao Liu; Su-Ying Ding; Jun-Jie Tan; Zhen-Guo Liu; Ya-Wen Zou; Zhi-Gang Ren; Zu-Jiang Yu

doi:10.1186/s40779-022-00387-y

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Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up

RESEARCH | Updated：2023-05-10

- Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up
- Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up
- Military Medical Research 2023年10卷第2期页码：162-174
- Affiliations：
  
  1.Department of Infectious Diseases, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  2.Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
  3.Department of Infectious Diseases, Guangshan County People's Hospital, Guangshan County, Xinyang 465450, Henan, China
  4.Department of Breast Surgery, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450052, China
  5.Shanghai Mobio Biomedical Technology Co., Ltd, Shanghai 201111, China
  6.Health Management Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
- Author bio：
  
  * fccrenzg@zzu.edu.cn;
  johnyuem@zzu.edu.cn
- Funds：
  
  grants from the National Key Research and Development Program of China(2018YFC2000501);National Natural Science Foundation of China(U2004121;82070643;U1904164)
- DOI：10.1186/s40779-022-00387-y
  中图分类号：
- 纸质出版：2023-04
- Accepted：
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Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up[J]. Military Medical Research, 2023,10(2):162-174.

Cite this article as: Cui GY, Rao BC, Zeng ZH, Wang XM, Ren T, Wang HY, et al. Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up. Mil Med Res. 2022;9(1):32.
Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up[J]. Military Medical Research, 2023,10(2):162-174. DOI： 10.1186/s40779-022-00387-y.

Cite this article as: Cui GY, Rao BC, Zeng ZH, Wang XM, Ren T, Wang HY, et al. Characterization of oral and gut microbiome and plasma metabolomics in COVID-19 patients after 1-year follow-up. Mil Med Res. 2022;9(1):32. DOI： 10.1186/s40779-022-00387-y.

摘要

Abstract

Background:

Due to the outbreak and rapid spread of coronavirus disease 2019 (COVID-19)

more than 160 million patients have become convalescents worldwide to date. Significant alterations have occurred in the gut and oral microbiome and metabonomics of patients with COVID-19. However

it is unknown whether their characteristics return to normal after the 1-year recovery.

Methods:

We recruited 35 confirmed patients to provide specimens at discharge and 1 year later

as well as 160 healthy controls. A total of 497 samples were prospectively collected

including 219 tongue-coating

129 stool and 149 plasma samples. Tongue-coating and stool samples were subjected to 16S rRNA sequencing

and plasma samples were subjected to untargeted metabolomics testing.

Results:

The oral and gut microbiome and metabolomics characteristics of the 1-year convalescents were restored to a large extent but did not completely return to normal. In the recovery process

the microbial diversity gradually increased. Butyric acid-producing microbes and Bifidobacterium gradually increased

whereas lipopolysaccharide-producing microbes gradually decreased. In addition

sphingosine-1-phosphate

which is closely related to the inflammatory factor storm of COVID-19

increased significantly during the recovery process. Moreover

the predictive models established based on the microbiome and metabolites of patients at the time of discharge reached high efficacy in predicting their neutralizing antibody levels one year later.

Conclusions:

This study is the first to characterize the oral and gut microbiome and metabonomics in 1-year convalescents of COVID-19. The key microbiome and metabolites in the process of recovery were identified

and provided new treatment ideas for accelerating recovery. And the predictive models based on the microbiome and metabolomics afford new insights for predicting the recovery situation which benefited affected individuals and healthcare.

关键词

Keywords

references

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