Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats

Lu-Yao Li; Jie Han; Lan Wu; Cheng Fang; Wei-Guang Li; Jia-Min Gu; Tong Deng; Chang-Jiang Qin; Jia-Yan Nie; Xian-Tao Zeng

doi:10.1186/s40779-022-00373-4

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Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats

RESEARCH | Updated：2023-03-23

- Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats
- Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats
- 解放军医学杂志（英文版） 2023年10卷第1期页码：1-16
- Affiliations：
  
  1.Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071 , China
  2.Department of Gastrointestinal Surgery, Huaihe Hospital of Henan University, Kaifeng 475000 , Henan, China
  3.Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan 430071 , China
  4.Department of Stomatology, Zhongnan Hospital of Wuhan University, Wuhan 430071 , China
  5.Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071 , China
- Author bio：
  
  * hhyyqcj@vip.henu.edu.cn;
  119140546@qq.com;
  zengxiantao1128 @163.com; zengxiantao1128@whu.edu.cn
- Funds：
  
  the Fundamental Research Funds for the Central Universities(2042021kf1041;2042021kf1041);the Medical Science and Technique Foundation of Henan Province(SBGJ202002097);the National Key Research and Development Plan of China (Technology helps Economy 2020)(2016YFC0106300)
- DOI：10.1186/s40779-022-00373-4
  中图分类号：
- 纸质出版：2023-02
- Accepted：
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Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats[J]. 解放军医学杂志（英文版）, 2023,10(1):1-16.

Li LY, Han J, Wu L, Fang C, Li WG, Gu JM, et al. Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats. Mil Med Res. 2022;9(1):12.
Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats[J]. 解放军医学杂志（英文版）, 2023,10(1):1-16. DOI： 10.1186/s40779-022-00373-4.

Li LY, Han J, Wu L, Fang C, Li WG, Gu JM, et al. Alterations of gut microbiota diversity, composition and metabonomics in testosterone-induced benign prostatic hyperplasia rats. Mil Med Res. 2022;9(1):12. DOI： 10.1186/s40779-022-00373-4.

摘要

Abstract

Background:

Studies had shown many diseases affect the stability of human microbiota

but how this relates to benign prostatic hyperplasia (BPH) has not been well understood. Hence

this study aimed to investigate the regulation of BPH on gut microbiota composition and metabonomics.

Methods:

We analyzed gut samples from rats with BPH and healthy control rats

the gut microbiota composition and metabonomics were detected by 16S rDNA sequencing and liquid chromatography tandem mass spectrometry (LC–MS/MS).

Results:

High-throughput sequencing results showed that gut microbiota beta-diversity increased (

0.01) in the BPH group

. control group.

Muribaculaceae

(

0.01)

Turicibacteraceae

(

0.05)

Turicibacter

(

0.01) and

Coprococcus

(

0.01) were significantly decreased in the BPH group

whereas that of

Mollicutes

(

0.05) and

Prevotella

(

0.05) were significantly increased compared with the control group. Despite profound interindividual variability

the levels of several predominant genera were different. In addition

there were no statistically significant differences in several bacteria. BPH group

. control group:

Firmicutes

(52.30%

. 57.29%

0.05)

Bacteroidetes

(46.54%

. 41.64%

0.05)

Clostridia

(50.89%

. 54.66%

0.05)

Ruminococcaceae

(25.67%

. 20.56%

0.05). LC–MS/MS of intestinal contents revealed that differential metabolites were mainly involved in cellular processes

environmental information processing

metabolism and organismal systems. The most important pathways were global and overview maps

lipid metabolism

amino acid metabolism

digestive system and endocrine system. Through enrichment analysis

we found that the differential metabolites were significantly enriched in metabolic pathways

steroid hormone biosynthesis

ovarian steroidogenesis

biosynthesis of unsaturated fatty acids and bile secretion. Pearson correlation analysis (

=0.94) showed that there was a strong correlation between

Prevotellaceae

Corynebacteriaceae

Turicibacteraceae

Bifidobacteriaceae

and differential metabolites.

Conclusions:

Our findings suggested an association between the gut microbiota and BPH

but the causal relationship between the two groups is unclear. Thus

further studies are warranted to elucidate the potential mechanisms and causal relationships between BPH and gut microbiota.

关键词

Keywords

references

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