Autologous omentum transposition for regeneration of a renal injury model in rats

Tayfun Bilgiç; Ümit İnce; Fehmi Narter

doi:10.1186/s40779-021-00361-0

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Autologous omentum transposition for regeneration of a renal injury model in rats

RESEARCH | Updated：2023-02-28

- Autologous omentum transposition for regeneration of a renal injury model in rats
- Autologous omentum transposition for regeneration of a renal injury model in rats
- 解放军医学杂志（英文版） 2022年9卷第5期页码：534-542
- Affiliations：
  
  1.Acıbadem Kadıkoy Hospital of General Surgery, Istanbul 34718, Turkey
  2.Department of Pathology, Acıbadem Mehmet ali Aydınlar University, Istanbul 34684, Turkey
  3.Department of Urology, Acıbadem Mehmet ali Aydınlar University, Istanbul 34684, Turkey
- Author bio：
  
  * tbilgic77@gmail.com
- Funds：
  
  the Acıbadem Mehmet ali Aydınlar University(under Grant 2018-03-05)
- DOI：10.1186/s40779-021-00361-0
  中图分类号：
- 纸质出版：2022-10
- Accepted：
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Autologous omentum transposition for regeneration of a renal injury model in rats[J]. 解放军医学杂志（英文版）, 2022,9(5):534-542.

Bilgiç T, İnce Ü, Narter F. Autologous omentum transposition for regeneration of a renal injury model in rats. Mil Med Res. 2022;9(1):1.
Autologous omentum transposition for regeneration of a renal injury model in rats[J]. 解放军医学杂志（英文版）, 2022,9(5):534-542. DOI： 10.1186/s40779-021-00361-0.

Bilgiç T, İnce Ü, Narter F. Autologous omentum transposition for regeneration of a renal injury model in rats. Mil Med Res. 2022;9(1):1. DOI： 10.1186/s40779-021-00361-0.

摘要

Abstract

Background:

After renal trauma

surgical treatment is vital

but sometimes there may be loss of function due to fibrosis. This study aimed to evaluate the effect of autologous omentum flaps on injured renal tissues in a rat model.

Methods:

A total of 30 Wistar albino rats were included and randomly divided equally into a control group and four intervention groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 group and primary repair 2 group) or transposition of the autologous omentum (omentum repair 1 group and omentum repair 2 group). Blood samples were taken preoperatively and on the 1st and 7th postoperative days in all groups and on the 18th postoperative day in the control and two intervention groups. All rats were sacrificed on the 7th or 18th day postoperatively

and their right kidneys were taken for histopathological evaluation.

Results:

The mean urea level significantly decreased from day 1 to day 7 and from day 1 to day 18 in the omentum repair 2 group (

=0.005 and

=0.004

respectively). There were no other significant changes in urea or creatinine levels within the intervention groups (P

0.05). There was no significant correlation between the urea and creatinine levels and the histological scores (P

0.05). The primary repair 1 and 2 groups had significantly higher median granulation and inflammation scores in the kidney specimen than the control and omentum repair groups (

0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (

0.05). The completion score for the healing process in the kidney specimen was significantly higher in the omentum repair groups than in the primary repair groups (

0.05). Granulation degree in the kidney specimen was strongly and positively correlated with the inflammation degree (

=0.824

0.001) and foreign body reaction in the kidney specimen (

=0.872

0.001)

and a strong and negative correlation with the healing process completion score in the kidney specimen (

=–0.627

=0.001). Inflammation degree in the kidney specimen was strongly and positively correlated with the foreign body reaction in the kidney specimen (

=0.731

=0.001)

and strongly and negatively correlated with the healing process completion score in the kidney specimen (

=–0.608

=0.002).

Conclusions:

Autologous omentum tissue for kidney injury repair attenuated inflammation and granulation. Additionally

the use of omental tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process with reduced fibrosis

tissue and function loss.

关键词

Keywords

references

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