Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China

Yi Wu; Xian-Hui Wang; Xi-Hua Li; Li-Yuan Song; Shi-Long Yu; Zhi-Cheng Fang; Yu-Quan Liu; Le-Yong Yuan; Chun-Yan Peng; Shen-Yi Zhang; Wang Cheng; Hong-Chao Ma; Li-Feng Wang; Jun-Ming Tang; Yun-Fu Wang; Fu-Yun Ji

doi:10.1186/s40779-021-00351-2

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Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China

RESEARCH | Updated：2023-02-28

- Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China
- Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China
- 解放军医学杂志（英文版） 2022年9卷第1期页码：53-62
- Affiliations：
  
  1.Department of Medical Biology, School of Basic Medical Science, Hubei University of Medicine, Shiyan 442000, Hubei, China
  2.Institute of Biomedical Research, Hubei University of Medicine, Shiyan 442000, Hubei, China
  3.Institute of Human Respiratory Disease, Xinqiao Hospital, The Army Medical University (Third Military Medical University), 400037 Chongqing, China
  4.Department of Emergency Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China
  5.Department of Geriatric Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China
  6.Department of Immunology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, Hubei, China
  7.Department of Laboratory Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China
  8.Hubei Key Laboratory of Embryonic Stem Cell Research, School of Basic Medical Science, Hubei University of Medicine, Shiyan 442000, Hubei, China
  9.Department of Neurology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China
- Author bio：
  
  * tangjm416@163.com;
  wangyunfu@hbmu.edu.cn;
  jifuyun@263.net; jfuyun2018@hbmu.edu.cn
- Funds：
  
  grants from the Special Project of Contingency Research for COVID-19(2020XGFYZR11);the Cultivating Project for Young Scholar at Hubei University of Medicine(2018QDJZR01);the Special Project of Contingency Research for COVID-19 at Hubei University of Medicine(2020XGFYZR03);The funders had no role in study design, collection, analysis and interpretation of data, writing of the manuscript and in decision to submit the article for publication
- DOI：10.1186/s40779-021-00351-2
  中图分类号：
- 纸质出版：2022-02
- Accepted：
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Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China[J]. 解放军医学杂志（英文版）, 2022,9(1):53-62.

Wu et al.: Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China. Mil Med Res, 2021, 8: 57.
Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China[J]. 解放军医学杂志（英文版）, 2022,9(1):53-62. DOI： 10.1186/s40779-021-00351-2.

Wu et al.: Common mtDNA variations at C5178a and A249d/T6392C/G10310A decrease the risk of severe COVID-19 in a Han Chinese population from Central China. Mil Med Res, 2021, 8: 57. DOI： 10.1186/s40779-021-00351-2.

摘要

Abstract

Background:

Mitochondria have been shown to play vital roles during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and coronavirus disease 2019 (COVID-19) development. Currently

it is unclear whether mitochondrial DNA (mtDNA) variants

which define mtDNA haplogroups and determine oxidative phosphorylation performance and reactive oxygen species production

are associated with COVID-19 risk.

Methods:

A population-based case-control study was conducted to compare the distribution of mtDNA variations defining mtDNA haplogroups between healthy controls (

=615) and COVID-19 patients (

=536). COVID-19 patients were diagnosed based on molecular diagnostics of the viral genome by qPCR and chest X-ray or computed tomography scanning. The exclusion criteria for the healthy controls were any history of disease in the month preceding the study assessment. MtDNA variants defining mtDNA haplogroups were identified by PCR-RFLPs and HVS-I sequencing and determined based on mtDNA phylogenetic analysis using Mitomap Phylogeny. Student’s

-test was used for continuous variables

and Pearson’s chi-squared test or Fisher’s exact test was used for categorical variables. To assess the independent effect of each mtDNA variant defining mtDNA haplogroups

multivariate logistic regression analyses were performed to calculate the odds ratios (

) and 95% confidence intervals (CI) with adjustments for possible confounding factors of age

sex

smoking and diseases (including cardiopulmonary diseases

diabetes

obesity and hypertension) as determined through clinical and radiographic examinations.

Results:

Multivariate logistic regression analyses revealed that the most common investigated mtDNA variations (

10% in the control population) at C5178a (in NADH dehydrogenase subunit 2 gene

ND2) and A249d (in the displacement loop region

D-loop)/T6392C (in cytochrome c oxidase I gene

CO1

)/G10310A (in

ND3

) were associated with a reduced risk of severe COVID-19 (

=0.590

95%CI 0.428–0.814

=0.001; and

=0.654

95%CI 0.457–0.936

=0.020

respectively)

while A4833G (ND2)

A4715G (ND2)

T3394C (ND1) and G5417A (ND2)/C16257a (D-loop)/C16261T (D-loop) were related to an increased risk of severe COVID-19 (

=2.336

95%CI 1.179–4.608

=0.015;

=2.033

95%CI 1.242–3.322

=0.005;

=3.040

95%CI 1.522–6.061

=0.002; and

=2.890

95%CI 1.199–6.993

=0.018

respectively).

Conclusions:

This is the first study to explore the association of mtDNA variants with individual’s risk of developing severe COVID-19. Based on the case–control study

we concluded that the common mtDNA variants at C5178a and A249d/T6392C/G10310A might contribute to an individual’s resistance to developing severe COVID-19

whereas A4833G

A4715G

T3394C and G5417A/C16257a/C16261T might increase an individual’s risk of developing severe COVID-19.

关键词

Keywords

references

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