DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia

Gang Xu; Yi-Kun Shi; Bin-Da Sun; Lu Liu; Guo-Ji E; Shu He; Jian-Yang Zhang; Bao Liu; Qiu Hu; Jian Chen; Yu-Qi Gao; Er-Long Zhang

doi:10.1186/s40779-021-00314-7

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DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia

RESEARCH | Updated：2023-02-28

- DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia
- DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia
- 解放军医学杂志（英文版） 2022年9卷第1期页码：1-11
- Affiliations：
  
  1.Institute of Medicine and Equipment for High Altitude Region, College of High Altitude Military Medicine, Army Medical University, Number 30, Gaotanyan Street, District of Shapingba, Chongqing 400038, China
  2.Key Laboratory of Extreme Environmental Medicine, Ministry of Education of China, Chongqing 400038, China
  3.Key Laboratory of High Altitude Medicine, People’s Liberation Army, Chongqing 400038, China
- Author bio：
  
  * gaoy66@yahoo.com;
  loong0810@sina.com
- Funds：
  
  grants from the National Science and Technology Major Project(2014ZX09J14102-05B;2018ZX09J18109)
- DOI：10.1186/s40779-021-00314-7
  中图分类号：
- 纸质出版：2022-02
- Accepted：
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DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia[J]. 解放军医学杂志（英文版）, 2022,9(1):1-11.

Xu et al.: DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia. Mil Med Res, 2021, 8: 23.
DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia[J]. 解放军医学杂志（英文版）, 2022,9(1):1-11. DOI： 10.1186/s40779-021-00314-7.

Xu et al.: DL-3-n-butylphthalide improved physical and learning and memory performance of rodents exposed to acute and chronic hypobaric hypoxia. Mil Med Res, 2021, 8: 23. DOI： 10.1186/s40779-021-00314-7.

摘要

Abstract

Background:

Studies have revealed the protective effect of DL-3-n-butylphthalide (NBP) against diseases associated with ischemic hypoxia. However

the role of NBP in animals with hypobaric hypoxia has not been elucidated. This study investigated the effects of NBP on rodents with acute and chronic hypobaric hypoxia.

Methods:

Sprague-Dwaley rats and Kunming mice administered with NBP (0

120

and 240 mg/kg for rats and 0

180

and 360 mg/kg for mice) were placed in a hypobaric hypoxia chamber at 10

000 m and the survival percentages at 30 min were determined. Then

the time and distance to exhaustion of drug-treated rodents were evaluated during treadmill running and motor-driven wheel-track treadmill experiments

conducted at 5800 m for 3 days or 20 days

to evaluate changes in physical functions. The frequency of active escapes and duration of active escapes were also determined for rats in a shuttle-box experiment

conducted at 5800 m for 6 days or 27 days

to evaluate changes in learning and memory function. ATP levels were measured in the gastrocnemius muscle and malonaldehyde (MDA)

superoxide dismutase (SOD)

hydrogen peroxide (H

)

glutathione peroxidase (GSH-Px)

and lactate were detected in sera of rats

and routine blood tests were also performed.

Results:

Survival analysis at 10

000 m indicated NBP could improve hypoxia tolerance ability. The time and distance to exhaustion for mice (NBP

90 mg/kg) and time to exhaustion for rats (NBP

120 and 240 mg/kg) significantly increased under conditions of acute hypoxia compared with control group. NBP treatment also significantly increased the time to exhaustion for rats when exposed to chronic hypoxia. Moreover

240 mg/kg NBP significantly increased the frequency of active escapes under conditions of acute hypoxia. Furthermore

the levels of MDA and H

decreased but those of SOD and GSH-Px in the sera of rats increased under conditions of acute and chronic hypoxia. Additionally

ATP levels in the gastrocnemius muscle significantly increased

while lactate levels in sera significantly decreased.

Conclusion:

NBP improved physical and learning and memory functions in rodents exposed to acute or chronic hypobaric hypoxia by increasing their anti-oxidative capacity and energy supply.

关键词

Keywords

references

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