Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of
Trichosporon asahii infectionComprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of
Trichosporon asahii infection- 解放军医学杂志（英文版） 2021年8卷第4期 页码：523-534
- Affiliations：
1.Department of Dermatology, Southwest Hospital, Army Medical University, Chongqing 400038, China
2.Department of Dermatology, the Seventh Medical Center of PLA General Hospital, 5 Nanmencang, Dongcheng District, Beijing 100700, China
3.Department of Ophthalmology, Hainan Hospital of Chinese PLA General Hospital, Sanya 572000, Hainan, China
4.The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510280, Guangdong, China
- Author bio：
* yangrya@sina.com
- Funds：the National Natural Science Foundation of China(81571972;81471928;81772138;82002120);the National Science Foundation of Beijing, China(7202201)
DOI：10.1186/s40779-021-00311-w
中图分类号：-
纸质出版：2021-12
- Accepted：
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Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of
Trichosporon asahii infection[J]. 解放军医学杂志（英文版）, 2021,8(4):523-534.Zhang et al.: Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of Trichosporon asahii infection. Mil Med Res, 2021, 8: 19.
Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of
Trichosporon asahii infection[J]. 解放军医学杂志（英文版）, 2021,8(4):523-534. DOI： 10.1186/s40779-021-00311-w.Zhang et al.: Comprehensive circRNA-microRNA-mRNA network analysis revealed the novel regulatory mechanism of Trichosporon asahii infection. Mil Med Res, 2021, 8: 19. DOI： 10.1186/s40779-021-00311-w.
摘要
Abstract
Background:
2
Invasive
Trichosporon asahii
(
T. asahii
) infection frequently occurs with a high mortality in immunodeficient hosts
but the pathogenesis of
T. asahii
infection remains elusive. Circular RNAs (circRNAs) are a type of endogenous noncoding RNA that participate in various disease processes. However
the mechanism of circRNAs in
T. asahii
infection remains completely unknown.
Methods:
2
RNA sequencing (RNA-seq) was performed to analyze the expression profiles of circRNAs
microRNAs (miRNAs)
and mRNAs in THP-1 cells infected with
T. asahii
or uninfected samples. Some of the RNA-seq results were verified by RT-qPCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the differentially expressed mRNAs. A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments.
Results:
2
A total of 46 circRNAs
412 mRNAs and 47 miRNAs were differentially expressed at 12 h after
T. asahii
infection. GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response
the Toll-like receptor signaling pathway
and the TNF signaling pathway. A competing endogenous RNA (ceRNA) network was constructed with 5 differentially expressed circRNAs
5 differentially expressed miRNAs and 42 differentially expressed mRNAs. Among them
hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p.
Conclusions:
2
These data revealed a comprehensive circRNA-associated ceRNA network during
T. asahii
infection
thus providing new insights into the pathogenesis of the
T. asahii
-host interactions.
关键词
Keywords
references
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