Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Shuai Wei; Wei Wang; Li Li; Hao-Ye Meng; Chun-Zhen Feng; Yu-Ying Dong; Xi-Chi Fang; Qi-Qiang Dong; Wen Jiang; Hai-Li Xin; Zhan-Zhen Li; Xin Wang

doi:10.1186/s40779-021-00308-5

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Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

RESEARCH | Updated：2023-02-28

- Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs
- Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs
- 解放军医学杂志（英文版） 2021年8卷第4期页码：510-522
- Affiliations：
  
  1.Institute of Orthopaedics, Beijing Key Lab of Regenerative Medicine in Orthopaedics, Chinese PLA General Hospital, Beijing 100583, China
  2.Zhoushan Dinghai Guanghua Hospital, Zhoushan 316000, Zhejiang, China
  3.Tianjin Hospital, Tianjin University, Tianjin 300211, China
  4.Geriatric Neurological Department of the Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing 100853, China
  5.Department of Orthopedics, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830000, China
  6.Department of Stomatology, Chinese PLA General Hospital, Beijing 100853, China
  7.Department of Plastic Surgery, General Hospital of Taiyuan Iron and Steel Limited Company, Taiyuan 030009, China
  8.Hand Microsurgery Department, Shenzhen People’s Hospital, Shenzhen 518020, Guangdong, China
  9.Third Surgery Department, Zhengzhou Renji Hospital, Zhengzhou 450000, China
  10.Department of Orthopedics, the First Affiliated Hospital of Medical College, Shihezi University, Shihezi, Xinjiang 832000, China
  11.Pharmacy Department, Chinese PLA General Hospital, Beijing 100853, China
- Author bio：
  
  * lizz2688@163.com;
  wangx126@126.com
- Funds：
  
  the National Natural Science Foundation of China(81972047;81603008;81572148)
- DOI：10.1186/s40779-021-00308-5
  中图分类号：
- 纸质出版：2021-12
- Accepted：
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Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs[J]. 解放军医学杂志（英文版）, 2021,8(4):510-522.

Wei et al.: Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs. Mil Med Res, 2021, 8: 18.
Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs[J]. 解放军医学杂志（英文版）, 2021,8(4):510-522. DOI： 10.1186/s40779-021-00308-5.

Wei et al.: Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs. Mil Med Res, 2021, 8: 18. DOI： 10.1186/s40779-021-00308-5.

摘要

Abstract

Background:

Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effects of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.

Methods:

We tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations (0

and 100 ng/ml) and different EGF action times (2

and 30 min). A full-thickness skin defect model was established using male

30-week-old Bama pigs. The experiment included groups as follows: routine dressing change after covering with sterile auxiliary material (Control)

continuous negative pressure drainage of the wound (VSD)

continuous negative pressure drainage of the wound and injection of EGF 10 min followed by removal by continuous lavage (V+E 10 min)

and continuous negative pressure drainage of the wound and injection of EGF 30 min followed by removal by continuous lavage (V+E 30 min). The wound healing rate

histological repair effect and collagen deposition were compared among the four groups.

Results:

An EGF concentration of 10 ng/ml and an action time of 10 min had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells. The drug dispersion effect was better than drug infusion after bolus injection effect

and the contact surface was wider. Compared with other groups

the V+E 10 min group promoted wound healing to the greatest extent and obtained the best histological score.

Conclusions:

A recombinant human epidermal growth factor (rhEGF) concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent

in vitro

. VSD combined with rhEGF kept in place for 10 min and then washed

can promote wound healing better than the other treatments

in vitro

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Keywords

references

Jiang B , Liang S , Peng ZR , Cong H , Levy M , Cheng Q , et al . Transport and public health in China: the road to a healthy future . Lancet . 2017 ; 390 ( 10104 ): 1781 - 91 .

Mam L , Kenter SB , Au FL , Wjm VG , Middelkoop E , Bos JD . Fibroblasts derived from chronic diabetic ulcers differ in their response to stimulation with EGF, IGF-I, bFGF and PDGF-AB compared to controls . Eur J Cell Biol . 2002 ; 81 ( 3 ): 153 - 60 .

Singer AJ , Clark RA . Cutaneous wound healing . N Engl J Med . 1999 ; 341 ( 10 ): 738 - 46 .

Xu P , Wu Y , Zhou L , Yang Z , Zhang X , Hu X , et al . Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization . Burns Trauma . 2020 ; 8 : tkaa028 .

Greenhalgh DG . The role of growth factors in wound healing . J Trauma . 1996 ; 41 ( 1 ): 159 - 67 .

Dwivedi C , Pandey H , Pandey AC , Patil S , Ramteke PW , Laux P , et al . In vivo biocompatibility of electrospun biodegradable dual carrier (antibiotic + growth factor) in a mouse model-implications for rapid wound healing . Pharmaceutics . 2019 ; 11 ( 4 ): 180 .

Dwivedi C , Pandey I , Pandey H , Patil S , Mishra SB , Pandey AC , et al . In vivo diabetic wound healing with nanofibrous scaffolds modified with gentamicin and recombinant human epidermal growth factor . J Biomed Mater Res A . 2018 ; 106 ( 3 ): 641 - 51 .

Fleischmann W , Strecker W , Bombelli M , Kinzl L . Vacuum sealing as treatment of soft tissue damage in open fractures . Unfallchirurg . 1993 ; 96 ( 9 ): 488 - 92 .

Witkowski W , Jawien A , Witkiewicz W , Zon B . Initial multi-Centre observations upon the effect of a new topical negative pressure device upon patient and clinician experience and the treatment of wounds . Int Wound J . 2009 ; 6 ( 2 ): 167 - 74 .

Scherer LA , Shiver S , Chang M , Meredith JW , Owings JT . The vacuum assisted closure device: a method of securing skin grafts and improving graft survival . Arch Surg . 2002 ; 137 ( 8 ): 930–3 discussion 3-4 .

Govea-Camacho LH , Astudillo-Carrera A , Hermosillo-Sandoval JM , Rodriguez-Reynoso S , Gonzalez-Ojeda A , Fuentes-Orozco C . Impact of vacuum-assisted closure management in deep neck abscesses . Cir Cir . 2016 ; 84 ( 4 ): 275 - 81 .

Beltzer C , Eisenächer A , Badendieck S , Doll D , Küper M , Lenz S , et al . Retrospective analysis of a VACM (vacuum-assisted closure and meshmediated fascial traction) treatment manual for temporary abdominal wall closure - results of 58 consecutive patients . Gms Interdiscip Plast Reconstr Surg Dgpw . 2016 ; 5 : Doc19 .

Suh H , Lee AY , Park EJ , Hong JP . Negative pressure wound therapy on closed surgical wounds with dead space: animal study using a swine model . Ann Plast Surg . 2016 ; 76 ( 6 ): 717 - 22 .

Singh AV , Gemmate D , Kanase A , Pandey I , Misra V , Kishore V , et al . Nanobiomaterials for vascular biology and wound management: a review . Veins Lymphatics . 2018 ; 7 : 7196 .

Milcheski DA , Chang AA , Lobato RC , Nakamoto HA , Tuma P Jr , Ferreira MC . Coverage of deep cutaneous wounds using dermal template in combination with negative-pressure therapy and subsequent skin graft . Plast Reconstr Surg Glob Open . 2014 ; 2 ( 6 ): e170 .

Poon H , Le Cocq H , Mountain AJ , Sargeant ID . Dermal fenestration with negative pressure wound therapy: a technique for managing soft tissue injuries associated with high-energy complex foot fractures . J Foot Ankle Surg . 2016 ; 55 ( 1 ): 161 - 5 .

Nanney LB . Epidermal and dermal effects of epidermal growth factor during wound repair . J Invest Dermatol . 1990 ; 94 ( 5 ): 624 - 9 .

Memon AA , Sundquist K , Ahmad A , Wang X , Hedelius A , Sundquist J . Role of IL-8, CRP and epidermal growth factor in depression and anxiety patients treated with mindfulness-based therapy or cognitive behavioral therapy in primary health care . Psychiatry Res . 2017 ; 254 : 311 - 6 .

Guo XF , Zhu XF , Cao HY , Zhong GS , Li L , Deng BG , et al . A bispecific enediyneenergized fusion protein targeting both epidermal growth factor receptor and insulin-like growth factor 1 receptor showing enhanced antitumor efficacy against non-small cell lung cancer . Oncotarget . 2017 ; 8 ( 16 ): 27286 - 99 .

Von Zastrow M , Sorkin A . Signaling on the endocytic pathway . Curr Opin Cell Biol . 2007 ; 19 ( 4 ): 436 - 45 .

Gomez-Villa R , Aguilar-Rebolledo F , Lozano-Platonoff A , Teran-Soto JM , Fabian-Victoriano MR , Kresch-Tronik NS , et al . Efficacy of intralesional recombinant human epidermal growth factor in diabetic foot ulcers in Mexican patients: a randomized double-blinded controlled trial . Wound Repair Regen . 2014 ; 22 ( 4 ): 497 - 503 .

Singla S , Garg R , Kumar A , Gill C . Efficacy of topical application of beta urogastrone (recombinant human epidermal growth factor) in Wagner's grade 1 and 2 diabetic foot ulcers: comparative analysis of 50 patients . J Nat Sci Biol Med . 2014 ; 5 ( 2 ): 273 .

Choi JS , Leong KW , Yoo HS . In vivo wound healing of diabetic ulcers using electrospun nanofibers immobilized with human epidermal growth factor (EGF) . Biomaterials . 2008 ; 29 ( 5 ): 587 - 96 .

Chouhan D , Chakraborty B , Nandi SK , Mandal BB . Role of non-mulberry silk fibroin in deposition and regulation of extracellular matrix towards accelerated wound healing . Acta Biomater . 2017 ; 48 : 157 - 74 .

Gonzalez G , Crombet T , Catala M , Mirabal V , Hernandez JC , Gonzalez Y , et al . A novel cancer vaccine composed of human-recombinant epidermal growth factor linked to a carrier protein: report of a pilot clinical trial . Ann Oncol . 1998 ; 9 ( 4 ): 431 - 5 .

Sigismund S , Argenzio E , Tosoni D , Cavallaro E , Polo S , Di Fiore PP . Clathrinmediated internalization is essential for sustained EGFR signaling but dispensable for degradation . Dev Cell . 2009 ; 15 ( 2 ): 209 - 19 .

Sullivan TP , Eaglstein WH , Davis SC , Mertz P . The pig as a model for human wound healing . Wound Repair Regen . 2001 ; 9 ( 2 ): 66 - 76 .

Youngjin J , Daegu S , Sunyoung K , Junhyung K , Kihwan H . Experimental pig model of clinically relevant wound healing delay by intrinsic factors . Int Wound J . 2013 ; 10 ( 3 ): 295 - 305 .

Liang G , Fu W , Wang K . Analysis of t-test misuses and SPSS operations in medical research papers . Burns Trauma . 2019 ; 7 : 31 .

Yamakawa S , Hayashida K . Advances in surgical applications of growth factors for wound healing . Burns Trauma . 2019 ; 7 : 10 .

Cohen S . Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal . J Biol Chem . 1962 ; 237 : 1555 - 62 .

Márquez EB , De Ortueta D , Royo SB , Martínez-Carpio PA . Epidermal growth factor receptor in corneal damage: update and new insights from recent reports . Cutan Ocul Toxicol . 2011 ; 30 ( 1 ): 7 - 14 .

Tiaka EK , Papanas N , Manolakis AC , Georgiadis GS . Epidermal growth factor in the treatment of diabetic foot ulcers: an update . Perspect Vasc Surg Endovasc Ther . 2012 ; 24 ( 1 ): 37 - 44 .

Natan S , Koren Y , Shelah O , Goren S , Lesman A . Long-range mechanical coupling of cells in 3D fibrin gels . Mol Biol Cell . 2020 ; 31 ( 14 ): 1474 - 85 .

Singh AV , Kishore V , Santomauro G , Yasa O , Bill J , Sitti M . Mechanical coupling of puller and pusher active microswimmers influences motility . Langmuir . 2020 ; 36 ( 19 ): 5435 - 43 .

Kaksonen M , Toret CP , Drubin DG . Harnessing actin dynamics for clathrinmediated endocytosis . Nat Rev Mol Cell Biol . 2006 ; 7 ( 6 ): 404 - 14 .

Mukhopadhyay D , Riezman H . Proteasome-independent functions of ubiquitin in endocytosis and signaling . Science . 2007 ; 315 ( 5809 ): 201 - 5 .

Yannas IV , Burke JF . Design of an artificial skin. I. Basic design principles . J Biomed Mater Res . 1980 ; 14 ( 1 ): 65 - 81 .

Suzuki S , Matsuda K , Isshiki N , Tamada Y , Ikada Y . Experimental study of a newly developed bilayer artificial skin . Biomaterials . 1990 ; 11 ( 5 ): 356 - 60 .

Ojalvo AG , Acosta JB , Marí YM , Mayola MF , Pérez CV , Gutiérrez WS , et al . Healing enhancement of diabetic wounds by locally infiltrated epidermal growth factor is associated with systemic oxidative stress reduction . Int Wound J . 2017 ; 14 ( 1 ): 214 - 25 .

Cheng HT , Hsu YC , Wu CI . Efficacy and safety of negative pressure wound therapy for Szilagyi grade III peripheral vascular graft infection . Interact Cardiovasc Thorac Surg . 2014 ; 19 ( 6 ): 1048 - 52 .

Kim YS , Sung DK , Kong WH , Kim H , Hahn SK . Synergistic effects of hyaluronate - epidermal growth factor conjugate patch on chronic wound healing . Biomater Sci . 2018 ; 6 ( 5 ): 1020 - 30 .

Singh AV , Aditi AS , Gade WN , Vats T , Lenardi C , Milani P . Nanomaterials: new generation therapeutics in wound healing and tissue repair . Curr Nanoe . 2010 ; 6 ( 6 ): 577 - 86 .

Garcia-Orue I , Gainza G , Gutierrez FB , Aguirre JJ , Evora C , Pedraz JL , et al . Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications . Int J Pharm . 2017 ; 523 ( 2 ): 556 - 66 .

Singh AV , Subhashree L , Milani P , Gemmati D , Zamboni P . Interplay of iron metallobiology, metalloproteinases, and FXIII, and role of their gene variants in venous leg ulcer . Int J Low Extrem Wounds . 2010 ; 9 ( 4 ): 166 - 79 .

Hardwicke J , Schmaljohann D , Boyce D , Thomas D . Epidermal growth factor therapy and wound healing--past, present and future perspectives . Surgeon . 2008 ; 6 ( 3 ): 172 - 7 .

Chen RR , Mooney DJ . Polymeric growth factor delivery strategies for tissue engineering . Pharm Res . 2003 ; 20 ( 8 ): 1103 - 12 .

Brown GL , Nanney LB , Griffen J , Cramer AB , Yancey JM , Curtsinger LJ 3rd , et al . Enhancement of wound healing by topical treatment with epidermal growth factor . N Engl J Med . 1989 ; 321 ( 2 ): 76 - 9 .

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