Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

Guo-Xing You; Bing-Ting Li; Zhen Wang; Quan Wang; Ying Wang; Jing-Xiang Zhao; Lian Zhao; Hong Zhou

doi:10.1186/s40779-020-00282-4

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Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution

RESEARCH | Updated：2023-02-28

- Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution
- Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution
- 解放军医学杂志（英文版） 2021年8卷第2期页码：183-195
- Affiliations：
  
  Institute of Health Service and Transfusion Medicine, Bejing 100850, China
- Author bio：
  
  * zhaolian@bmi.ac.cn;
  zhouhtt1966@163.com
- Funds：
  
  the National Key Research and Development Program of China(2018YFC1106503)
- DOI：10.1186/s40779-020-00282-4
  中图分类号：
- 纸质出版：2021-06
- Accepted：
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Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution[J]. 解放军医学杂志（英文版）, 2021,8(2):183-195.

You et al.: Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution. Mil Med Res, 2020, 7: 55.
Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution[J]. 解放军医学杂志（英文版）, 2021,8(2):183-195. DOI： 10.1186/s40779-020-00282-4.

You et al.: Effects of different plasma expanders on rats subjected to severe acute normovolemic hemodilution. Mil Med Res, 2020, 7: 55. DOI： 10.1186/s40779-020-00282-4.

摘要

Abstract

Background:

Plasma expanders are widely used for acute normovolemic hemodilution (ANH). However

existing studies have not focused on large-volume infusion with colloidal plasma expanders

and there is a lack of studies that compare the effects of different plasma expanders.

Methods:

The viscosity

hydrodynamic radius (R

) and colloid osmotic pressure (COP) of plasma expanders were determined by a cone-plate viscometer

Zetasizer and cut-off membrane

respectively. Sixty male rats were randomized into five groups with Gelofusine (Gel)

Hydroxyethyl Starch 200/0.5 (HES200)

Hydroxyethyl Starch 130/0.4 (HES130)

Hydroxyethyl Starch 40 (HES40)

and Dextran 40 (Dex40)

with 12 rats used in each group to build the ANH model. ANH was performed by the withdrawal of blood and simultaneous infusion of plasma expanders. Acid-base

lactate

blood gas and physiological parameters were detected.

Results:

Gel had a lower intrinsic viscosity than HES200 and HES130 (

0.01)

but at a low shear rate in a mixture of colloids

red cells and plasma

Gel had a higher viscosity (

0.05 or

0.01

respectively). For hydroxyethyl starch plasma expanders

the COP at a certain concentration decreases from 11.1 mmHg to 6.1 mmHg with the increase of R

from 10.7 nm to 20.2 nm. A severe ANH model

with the hematocrit of 40% of the baseline level

was established and accompanied by disturbances in acid-base

lactate and blood gas parameters. At the end of ANH and 60 min afterward

the Dex40 group showed a worse outcome in maintaining the acid-base balance and systemic oxygenation compared to the other groups. The systolic blood pressure (SBP)

diastolic blood pressure (DBP)

and mean arterial pressure (MAP) decreased significantly in all groups at the end of ANH. The DBP and MAP in the Dex40 group further decreased 60 min after the end of ANH. During the process of ANH

the Dex40 group showed a drop and recovery in SBP

DBP and MAP. The DBP and MAP in the HES200 group were significantly higher than those in the other groups at some time points (

0.05 or

0.01).

Conclusions:

Gel had a low intrinsic viscosity but may increase the whole blood viscosity at low shear rates. R

and COP showed a strong correlation among hydroxyethyl starch plasma expanders. Dex40 showed a worse outcome in maintaining the acid-base balance and systemic oxygenation compared to the other plasma expanders. During the process of ANH

Dex40 displayed a V-shaped recovery pattern for blood pressure

and HES200 had the advantage in sustaining the DBP and MAP at some time points.

关键词

Keywords

references

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