Effects of
Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genesEffects of
Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes- MMR 2020年7卷第2期 页码：125-139
- Affiliations：
Army Tuberculosis Prevention and Control Key Laboratory/Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute for Tuberculosis Research, the 8th Medical Center of Chinese PLA General Hospital, Beijing 100091, China
- Author bio：
* xueqiongwu@139.com
- Funds：Grants from the National Natural Science Foundation of China(81801643);the National Key Program for Infectious Disease of China(2018ZX10731301–005);Beijing Municipal Science & Technology Commission(Z181100001718005);the Medical Science and Technology Youth Cultivation Program of PLA(16QNP075)
DOI：10.1186/s40779-020-00258-4
中图分类号：-
纸质出版：2020-06
- Accepted：
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Effects of
Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes[J]. MMR, 2020,7(2):125-139.Cite this article as: Gong et al.: Effects of Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes. Mil Med Res, 2020, 7: 25
Effects of
Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes[J]. MMR, 2020,7(2):125-139. DOI： 10.1186/s40779-020-00258-4.Cite this article as: Gong et al.: Effects of Mycobacterium vaccae vaccine in a mouse model of tuberculosis: protective action and differentially expressed genes. Mil Med Res, 2020, 7: 25 DOI： 10.1186/s40779-020-00258-4.
摘要
Abstract
Background:
2
Tuberculosis is a leading cause of death worldwide. BCG is an effective vaccine
but not widely used in many parts of the world due to a variety of issues.
Mycobacterium vaccae
(
M. vaccae
) is another vaccine used in human subjects to prevent tuberculosis. In the current study
we investigated the potential mechanisms of
M. vaccae
vaccination by determining differentially expressed genes in mice infected with
M. tuberculosis
before and after
M. vaccae
vaccination.
Methods:
2
Three days after exposure to
M. tuberculosis
H37Rv strain (5×10
5
CFU)
adult BALB/c mice randomly received either
M. vaccae
vaccine (22.5 μg) or vehicle
via
intramuscular injection (
n
=8). Booster immunization was conducted 14 and 28 days after the primary immunization. Differentially expressed genes were identified by microarray followed by standard bioinformatics analysis.
Results:
2
M. vaccae
vaccination provided protection against
M. tuberculosis
infection (most prominent in the lungs). We identified 2
326 upregulated and 2
221 downregulated genes in vaccinated mice. These changes could be mapped to a total of 123 signaling pathways (68 upregulated and 55 downregulated). Further analysis pinpointed to the MyD88-dependent TLR signaling pathway and PI3K-Akt signaling pathway as most likely to be functional.
Conclusions:
2
M. vaccae
vaccine provided good protection in mice against
M. tuberculosis
infection
via
a highly complex set of molecular changes. Our findings may provide clue to guide development of more effective vaccine against tuberculosis.
关键词
Keywords
references
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